Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/58064 http://dx.doi.org/10.3390/ph10040085 |
Resumo: | The administration of lithium-pilocarpine (LiPilo) in adult rats is a validated model reproducing the main clinical and neuropathological features of temporal lobe epilepsy (TLE). Previous studies have shown that carisbamate (CRS) has the property of modifying epileptogenesis in this model. When treated with CRS, about 50% of rats undergoing LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of convulsive ones (commonly observed in TLE). The goal of this work was to determine some of the early changes that occur after CRS administration, as they could be involved in the insult- and epileptogenesis-modifying effects of CRS. Thus, we performed high-performance liquid chromatography (HPLC) to quantify levels of amino acids and monoamines, and c-Fos immunohistochemical labeling to map cerebral activation during seizures. Comparing rats treated one hour after SE onset with saline (CT), CRS, or diazepam (DZP), HPLC showed that 4 h after SE onset, dopamine (DA), norepinephrine (NE), and GABA levels were normal, whereas serotonin levels were increased. Using c-Fos labeling, we demonstrated increased activity in thalamic mediodorsal (MD) and laterodorsal (LD) nuclei in rats treated with CRS. In summary, at early times, CRS seems to modulate excitability by acting on some monoamine levels and increasing activity of MD and LD thalamic nuclei, suggesting a possible involvement of these nuclei in insult- and/or epileptogenesis-modifying effects of CRS. |
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Marques-Carneiro, Jose Eduardo [UNIFESP]Nehlig, AstridCassel, Jean-ChristopheFerreira Castro-Neto, Eduardo [UNIFESP]Litzahn, Julia Julie [UNIFESP]de Vasconcelos, Anne PereiraNaffah-Mazacoratti, Maria da Graca [UNIFESP]da Silva Fernandes, Maria Jose [UNIFESP]2020-09-01T13:21:03Z2020-09-01T13:21:03Z2017Pharmaceuticals. Basel, v. 10, n. 4, p. -, 2017.1424-8247https://repositorio.unifesp.br/handle/11600/58064http://dx.doi.org/10.3390/ph10040085WOS000419241100009.pdf10.3390/ph10040085WOS:000419241100009The administration of lithium-pilocarpine (LiPilo) in adult rats is a validated model reproducing the main clinical and neuropathological features of temporal lobe epilepsy (TLE). Previous studies have shown that carisbamate (CRS) has the property of modifying epileptogenesis in this model. When treated with CRS, about 50% of rats undergoing LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of convulsive ones (commonly observed in TLE). The goal of this work was to determine some of the early changes that occur after CRS administration, as they could be involved in the insult- and epileptogenesis-modifying effects of CRS. Thus, we performed high-performance liquid chromatography (HPLC) to quantify levels of amino acids and monoamines, and c-Fos immunohistochemical labeling to map cerebral activation during seizures. Comparing rats treated one hour after SE onset with saline (CT), CRS, or diazepam (DZP), HPLC showed that 4 h after SE onset, dopamine (DA), norepinephrine (NE), and GABA levels were normal, whereas serotonin levels were increased. Using c-Fos labeling, we demonstrated increased activity in thalamic mediodorsal (MD) and laterodorsal (LD) nuclei in rats treated with CRS. In summary, at early times, CRS seems to modulate excitability by acting on some monoamine levels and increasing activity of MD and LD thalamic nuclei, suggesting a possible involvement of these nuclei in insult- and/or epileptogenesis-modifying effects of CRS.Coordenacao de Aperfeicoamento de Pessoal deNivel Superior-CAPES-BrasilFundacao de Amparo a Pesquisa no Estado de Sao Paulo-FAPESPConselho Nacional de Desenvolvimento Cientifico e Tecnologico-CNPqFundacao de Apoio a Unifesp-FAP-UNIFESP, BrazilUniv Fed Sao Paulo, Dept Neurol Neurocirurgia, Disciplina Neurociencia, BR-04021001 Sao Paulo, BrazilUniv Strasbourg, Fac Psychol, Lab Neurosci Cognit & Adaptat, Unistra, F-67000 Strasbourg, FranceCNRS, LNCA, UMR 7364, 12 Rue Goethe, F-67000 Strasbourg, FranceINSERM, U1129, Infantile Epilepsies & Brain Plast, F-75654 Paris, FranceUniv Paris 05, Sorbonne Paris Cite, F-91190 Gif Sur Yvette, FranceUniv Fed Sao Paulo, Dept Neurol Neurocirurgia, Disciplina Neurociencia, BR-04021001 Sao Paulo, BrazilWeb of Science-engMdpi AgPharmaceuticalscarisbamate 1temporal-lobe epilepsy 2brain activity 3Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBasel104info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000419241100009.pdfapplication/pdf2151062${dspace.ui.url}/bitstream/11600/58064/1/WOS000419241100009.pdf493da46c77957b697050fd461c3a9f22MD51open accessTEXTWOS000419241100009.pdf.txtWOS000419241100009.pdf.txtExtracted texttext/plain54040${dspace.ui.url}/bitstream/11600/58064/2/WOS000419241100009.pdf.txt5476b3a3ecd946d9724e6cdec75c7fd1MD52open accessTHUMBNAILWOS000419241100009.pdf.jpgWOS000419241100009.pdf.jpgIM Thumbnailimage/jpeg6742${dspace.ui.url}/bitstream/11600/58064/4/WOS000419241100009.pdf.jpg8c3fc5a111bf68b7f60aed9be8a65ebaMD54open access11600/580642022-07-31 19:06:54.512open accessoai:repositorio.unifesp.br:11600/58064Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-31T22:06:54Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
title |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
spellingShingle |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus Marques-Carneiro, Jose Eduardo [UNIFESP] carisbamate 1 temporal-lobe epilepsy 2 brain activity 3 |
title_short |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
title_full |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
title_fullStr |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
title_full_unstemmed |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
title_sort |
Neurochemical Changes and c-Fos Mapping in the Brain after Carisbamate Treatment of Rats Subjected to Lithium-Pilocarpine-Induced Status Epilepticus |
author |
Marques-Carneiro, Jose Eduardo [UNIFESP] |
author_facet |
Marques-Carneiro, Jose Eduardo [UNIFESP] Nehlig, Astrid Cassel, Jean-Christophe Ferreira Castro-Neto, Eduardo [UNIFESP] Litzahn, Julia Julie [UNIFESP] de Vasconcelos, Anne Pereira Naffah-Mazacoratti, Maria da Graca [UNIFESP] da Silva Fernandes, Maria Jose [UNIFESP] |
author_role |
author |
author2 |
Nehlig, Astrid Cassel, Jean-Christophe Ferreira Castro-Neto, Eduardo [UNIFESP] Litzahn, Julia Julie [UNIFESP] de Vasconcelos, Anne Pereira Naffah-Mazacoratti, Maria da Graca [UNIFESP] da Silva Fernandes, Maria Jose [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Marques-Carneiro, Jose Eduardo [UNIFESP] Nehlig, Astrid Cassel, Jean-Christophe Ferreira Castro-Neto, Eduardo [UNIFESP] Litzahn, Julia Julie [UNIFESP] de Vasconcelos, Anne Pereira Naffah-Mazacoratti, Maria da Graca [UNIFESP] da Silva Fernandes, Maria Jose [UNIFESP] |
dc.subject.eng.fl_str_mv |
carisbamate 1 temporal-lobe epilepsy 2 brain activity 3 |
topic |
carisbamate 1 temporal-lobe epilepsy 2 brain activity 3 |
description |
The administration of lithium-pilocarpine (LiPilo) in adult rats is a validated model reproducing the main clinical and neuropathological features of temporal lobe epilepsy (TLE). Previous studies have shown that carisbamate (CRS) has the property of modifying epileptogenesis in this model. When treated with CRS, about 50% of rats undergoing LiPilo status epilepticus (SE) develop non-convulsive seizures (NCS) instead of convulsive ones (commonly observed in TLE). The goal of this work was to determine some of the early changes that occur after CRS administration, as they could be involved in the insult- and epileptogenesis-modifying effects of CRS. Thus, we performed high-performance liquid chromatography (HPLC) to quantify levels of amino acids and monoamines, and c-Fos immunohistochemical labeling to map cerebral activation during seizures. Comparing rats treated one hour after SE onset with saline (CT), CRS, or diazepam (DZP), HPLC showed that 4 h after SE onset, dopamine (DA), norepinephrine (NE), and GABA levels were normal, whereas serotonin levels were increased. Using c-Fos labeling, we demonstrated increased activity in thalamic mediodorsal (MD) and laterodorsal (LD) nuclei in rats treated with CRS. In summary, at early times, CRS seems to modulate excitability by acting on some monoamine levels and increasing activity of MD and LD thalamic nuclei, suggesting a possible involvement of these nuclei in insult- and/or epileptogenesis-modifying effects of CRS. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-09-01T13:21:03Z |
dc.date.available.fl_str_mv |
2020-09-01T13:21:03Z |
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dc.identifier.citation.fl_str_mv |
Pharmaceuticals. Basel, v. 10, n. 4, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/58064 http://dx.doi.org/10.3390/ph10040085 |
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1424-8247 |
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WOS000419241100009.pdf |
dc.identifier.doi.none.fl_str_mv |
10.3390/ph10040085 |
dc.identifier.wos.none.fl_str_mv |
WOS:000419241100009 |
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Pharmaceuticals. Basel, v. 10, n. 4, p. -, 2017. 1424-8247 WOS000419241100009.pdf 10.3390/ph10040085 WOS:000419241100009 |
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https://repositorio.unifesp.br/handle/11600/58064 http://dx.doi.org/10.3390/ph10040085 |
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Mdpi Ag |
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Mdpi Ag |
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