Endurance training inhibits insulin clearance and IDE expression in Swiss mice
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38905 http://dx.doi.org/10.1371/journal.pone.0118809 |
Resumo: | IntroductionEndurance training improves peripheral insulin sensitivity in the liver and the skeletal muscle, but the mechanism for this effect is poorly understood. Recently, it was proposed that insulin clearance plays a major role in both glucose homeostasis and insulin sensitivity. Therefore, our goal was to determine the mechanism by which endurance training improves insulin sensitivity and how it regulates insulin clearance in mice.MethodsMice were treadmill-trained for 4 weeks at 70-80% of maximal oxygen consumption (VO2 max) for 60 min, 5 days a week. the glucose tolerance and the insulin resistance were determined using an IPGTT and an IPITT, respectively, and the insulin decay rate was calculated from the insulin clearance. Protein expression and phosphorylation in the liver and the skeletal muscle were ascertained by Western blot.ResultsTrained mice exhibited an increased VO2 max, time to exhaustion, glucose tolerance and insulin sensitivity. They had smaller fat pads and lower plasma concentrations of insulin and glucose. Endurance training inhibited insulin clearance and reduced expression of IDE in the liver, while also inhibiting insulin secretion by pancreatic islets. There was increased phosphorylation of both the canonical (IR-AKT) and the non-canonical (CaMKII-AMPK-ACC) insulin pathways in the liver of trained mice, whereas only the CaMKII-AMPK pathway was increased in the skeletal muscle.ConclusionEndurance training improved glucose homeostasis not only by increasing peripheral insulin sensitivity but also by decreasing insulin clearance and reducing IDE expression in the liver. |
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Costa-Junior, Jose M.Ferreira, Sandra M.Protzek, Andre O.Santos, Gustavo J.Cappelli, Ana P.Silveira, Leonardo R.Zoppi, ClaudioOliveira, Camila Aparecida Machado de [UNIFESP]Boschero, Antonio C.Carneiro, Everardo M.Rezende, Luiz F.Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:40:16Z2016-01-24T14:40:16Z2015-03-30Plos One. San Francisco: Public Library Science, v. 10, n. 3, 14 p., 2015.1932-6203http://repositorio.unifesp.br/handle/11600/38905http://dx.doi.org/10.1371/journal.pone.0118809WOS000352134700024.pdf10.1371/journal.pone.0118809WOS:000352134700024IntroductionEndurance training improves peripheral insulin sensitivity in the liver and the skeletal muscle, but the mechanism for this effect is poorly understood. Recently, it was proposed that insulin clearance plays a major role in both glucose homeostasis and insulin sensitivity. Therefore, our goal was to determine the mechanism by which endurance training improves insulin sensitivity and how it regulates insulin clearance in mice.MethodsMice were treadmill-trained for 4 weeks at 70-80% of maximal oxygen consumption (VO2 max) for 60 min, 5 days a week. the glucose tolerance and the insulin resistance were determined using an IPGTT and an IPITT, respectively, and the insulin decay rate was calculated from the insulin clearance. Protein expression and phosphorylation in the liver and the skeletal muscle were ascertained by Western blot.ResultsTrained mice exhibited an increased VO2 max, time to exhaustion, glucose tolerance and insulin sensitivity. They had smaller fat pads and lower plasma concentrations of insulin and glucose. Endurance training inhibited insulin clearance and reduced expression of IDE in the liver, while also inhibiting insulin secretion by pancreatic islets. There was increased phosphorylation of both the canonical (IR-AKT) and the non-canonical (CaMKII-AMPK-ACC) insulin pathways in the liver of trained mice, whereas only the CaMKII-AMPK pathway was increased in the skeletal muscle.ConclusionEndurance training improved glucose homeostasis not only by increasing peripheral insulin sensitivity but also by decreasing insulin clearance and reducing IDE expression in the liver.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, BR-13083865 Campinas, SP, BrazilUniv São Paulo, Dept Biochem & Immunol, Fac Med Ribeirao Preto, Ribeirao Preto, SP, BrazilFed Univ São Paulo Unifesp, Dept Biosci, BR-11060001 Santos, SP, BrazilFed Univ São Paulo Unifesp, Dept Biosci, BR-11060001 Santos, SP, BrazilWeb of Science14engPublic Library SciencePlos OneEndurance training inhibits insulin clearance and IDE expression in Swiss miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000352134700024.pdfapplication/pdf748729${dspace.ui.url}/bitstream/11600/38905/1/WOS000352134700024.pdf35cf9f266d1878171a4d5e6a782bed6dMD51open accessTEXTWOS000352134700024.pdf.txtWOS000352134700024.pdf.txtExtracted texttext/plain41782${dspace.ui.url}/bitstream/11600/38905/6/WOS000352134700024.pdf.txtb0addf23350514f7f04052cafa3e816aMD56open accessTHUMBNAILWOS000352134700024.pdf.jpgWOS000352134700024.pdf.jpgIM Thumbnailimage/jpeg7044${dspace.ui.url}/bitstream/11600/38905/8/WOS000352134700024.pdf.jpg5989cdfe46b62086cb3d95fdbc5b8c83MD58open access11600/389052023-06-05 19:09:17.384open accessoai:repositorio.unifesp.br:11600/38905Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:09:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
title |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
spellingShingle |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice Costa-Junior, Jose M. |
title_short |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
title_full |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
title_fullStr |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
title_full_unstemmed |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
title_sort |
Endurance training inhibits insulin clearance and IDE expression in Swiss mice |
author |
Costa-Junior, Jose M. |
author_facet |
Costa-Junior, Jose M. Ferreira, Sandra M. Protzek, Andre O. Santos, Gustavo J. Cappelli, Ana P. Silveira, Leonardo R. Zoppi, Claudio Oliveira, Camila Aparecida Machado de [UNIFESP] Boschero, Antonio C. Carneiro, Everardo M. Rezende, Luiz F. |
author_role |
author |
author2 |
Ferreira, Sandra M. Protzek, Andre O. Santos, Gustavo J. Cappelli, Ana P. Silveira, Leonardo R. Zoppi, Claudio Oliveira, Camila Aparecida Machado de [UNIFESP] Boschero, Antonio C. Carneiro, Everardo M. Rezende, Luiz F. |
author2_role |
author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Costa-Junior, Jose M. Ferreira, Sandra M. Protzek, Andre O. Santos, Gustavo J. Cappelli, Ana P. Silveira, Leonardo R. Zoppi, Claudio Oliveira, Camila Aparecida Machado de [UNIFESP] Boschero, Antonio C. Carneiro, Everardo M. Rezende, Luiz F. |
description |
IntroductionEndurance training improves peripheral insulin sensitivity in the liver and the skeletal muscle, but the mechanism for this effect is poorly understood. Recently, it was proposed that insulin clearance plays a major role in both glucose homeostasis and insulin sensitivity. Therefore, our goal was to determine the mechanism by which endurance training improves insulin sensitivity and how it regulates insulin clearance in mice.MethodsMice were treadmill-trained for 4 weeks at 70-80% of maximal oxygen consumption (VO2 max) for 60 min, 5 days a week. the glucose tolerance and the insulin resistance were determined using an IPGTT and an IPITT, respectively, and the insulin decay rate was calculated from the insulin clearance. Protein expression and phosphorylation in the liver and the skeletal muscle were ascertained by Western blot.ResultsTrained mice exhibited an increased VO2 max, time to exhaustion, glucose tolerance and insulin sensitivity. They had smaller fat pads and lower plasma concentrations of insulin and glucose. Endurance training inhibited insulin clearance and reduced expression of IDE in the liver, while also inhibiting insulin secretion by pancreatic islets. There was increased phosphorylation of both the canonical (IR-AKT) and the non-canonical (CaMKII-AMPK-ACC) insulin pathways in the liver of trained mice, whereas only the CaMKII-AMPK pathway was increased in the skeletal muscle.ConclusionEndurance training improved glucose homeostasis not only by increasing peripheral insulin sensitivity but also by decreasing insulin clearance and reducing IDE expression in the liver. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-03-30 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:40:16Z |
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2016-01-24T14:40:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 10, n. 3, 14 p., 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38905 http://dx.doi.org/10.1371/journal.pone.0118809 |
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1932-6203 |
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WOS000352134700024.pdf |
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10.1371/journal.pone.0118809 |
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Plos One. San Francisco: Public Library Science, v. 10, n. 3, 14 p., 2015. 1932-6203 WOS000352134700024.pdf 10.1371/journal.pone.0118809 WOS:000352134700024 |
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http://repositorio.unifesp.br/handle/11600/38905 http://dx.doi.org/10.1371/journal.pone.0118809 |
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