EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI
Autor(a) principal: | |
---|---|
Data de Publicação: | 1992 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/25252 http://dx.doi.org/10.1084/jem.175.6.1635 |
Resumo: | Trypomastigotes of Trypanosoma cruzi have to invade mammalian cells in order to multiply. They bear on their plasma membrane a sialic acid-containing epitope (Ssp-3) defined by a series of monoclonal antibodies (mAbs). Previous investigations have shown that Fab fragments of these mAbs inhibit the attachment of trypomastigotes to 3T3 fibroblasts. To further define the role of Ssp-3 in invasion, here we use, as targets for infection, L cells and CHO cells stably transfected with cDNA coding for the mouse Fc receptors genes. When the trypomastigotes are incubated with small, nonagglutinating amounts of antibodies to Ssp-3, their attachment to the transfected cells is greatly enhanced, without a parallel increase in invasion. the enhancement in attachment is Fc mediated, since it is abolished by treatment of the transfected cells with mAbs to Fc receptors. in contrast, both attachment to, and invasion of, the transfected cells are increased if the parasites are incubated with polyclonal or monoclonal antibodies against T. cruzi surface membrane antigens other than Ssp-3. If, however, antibodies to Ssp-3 are added to the incubation mixtures containing any of the other anti-T. cruzi antibodies, the enhancement of invasion (but not of attachment) is reversed. These results suggest that Ssp-3-bearing molecules participate in the process of parasite internalization. |
id |
UFSP_ab20e4d63599187f972cd7dd41e105aa |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/25252 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Schenkman, Sergio [UNIFESP]Kurosaki, T.Ravetch, J. V.Nussenzweig, V.NYU MED CTRSLOAN KETTERING MEM CANC CTRUniversidade Federal de São Paulo (UNIFESP)2016-01-24T11:40:06Z2016-01-24T11:40:06Z1992-06-01Journal of Experimental Medicine. New York: Rockefeller Univ Press, v. 175, n. 6, p. 1635-1641, 1992.0022-1007http://repositorio.unifesp.br/handle/11600/25252http://dx.doi.org/10.1084/jem.175.6.1635WOSA1992HV67600025.pdf10.1084/jem.175.6.1635WOS:A1992HV67600025Trypomastigotes of Trypanosoma cruzi have to invade mammalian cells in order to multiply. They bear on their plasma membrane a sialic acid-containing epitope (Ssp-3) defined by a series of monoclonal antibodies (mAbs). Previous investigations have shown that Fab fragments of these mAbs inhibit the attachment of trypomastigotes to 3T3 fibroblasts. To further define the role of Ssp-3 in invasion, here we use, as targets for infection, L cells and CHO cells stably transfected with cDNA coding for the mouse Fc receptors genes. When the trypomastigotes are incubated with small, nonagglutinating amounts of antibodies to Ssp-3, their attachment to the transfected cells is greatly enhanced, without a parallel increase in invasion. the enhancement in attachment is Fc mediated, since it is abolished by treatment of the transfected cells with mAbs to Fc receptors. in contrast, both attachment to, and invasion of, the transfected cells are increased if the parasites are incubated with polyclonal or monoclonal antibodies against T. cruzi surface membrane antigens other than Ssp-3. If, however, antibodies to Ssp-3 are added to the incubation mixtures containing any of the other anti-T. cruzi antibodies, the enhancement of invasion (but not of attachment) is reversed. These results suggest that Ssp-3-bearing molecules participate in the process of parasite internalization.NYU MED CTR,DEPT PATHOL,550 1ST AVE,NEW YORK,NY 10016NYU MED CTR,KAPLAN CANC CTR,NEW YORK,NY 10016SLOAN KETTERING MEM CANC CTR,DEWITT WALLACE LAB,NEW YORK,NY 10021ESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 São Paulo,BRAZILESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 São Paulo,BRAZILWeb of Science1635-1641engRockefeller Univ PressJournal of Experimental MedicineEVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZIinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOSA1992HV67600025.pdfapplication/pdf1470326${dspace.ui.url}/bitstream/11600/25252/1/WOSA1992HV67600025.pdf2ec2e73f2e4f9daeafb3c58f80e674e0MD51open accessTEXTWOSA1992HV67600025.pdf.txtWOSA1992HV67600025.pdf.txtExtracted texttext/plain28489${dspace.ui.url}/bitstream/11600/25252/2/WOSA1992HV67600025.pdf.txt2028237dc40148890460e58c2b40d0dcMD52open access11600/252522022-02-18 12:08:04.529open accessoai:repositorio.unifesp.br:11600/25252Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-18T15:08:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
title |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
spellingShingle |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI Schenkman, Sergio [UNIFESP] |
title_short |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
title_full |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
title_fullStr |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
title_full_unstemmed |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
title_sort |
EVIDENCE for the PARTICIPATION of the SSP-3 ANTIGEN in the INVASION of NONPHAGOCYTIC MAMMALIAN-CELLS BY TRYPANOSOMA-CRUZI |
author |
Schenkman, Sergio [UNIFESP] |
author_facet |
Schenkman, Sergio [UNIFESP] Kurosaki, T. Ravetch, J. V. Nussenzweig, V. |
author_role |
author |
author2 |
Kurosaki, T. Ravetch, J. V. Nussenzweig, V. |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
NYU MED CTR SLOAN KETTERING MEM CANC CTR Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Schenkman, Sergio [UNIFESP] Kurosaki, T. Ravetch, J. V. Nussenzweig, V. |
description |
Trypomastigotes of Trypanosoma cruzi have to invade mammalian cells in order to multiply. They bear on their plasma membrane a sialic acid-containing epitope (Ssp-3) defined by a series of monoclonal antibodies (mAbs). Previous investigations have shown that Fab fragments of these mAbs inhibit the attachment of trypomastigotes to 3T3 fibroblasts. To further define the role of Ssp-3 in invasion, here we use, as targets for infection, L cells and CHO cells stably transfected with cDNA coding for the mouse Fc receptors genes. When the trypomastigotes are incubated with small, nonagglutinating amounts of antibodies to Ssp-3, their attachment to the transfected cells is greatly enhanced, without a parallel increase in invasion. the enhancement in attachment is Fc mediated, since it is abolished by treatment of the transfected cells with mAbs to Fc receptors. in contrast, both attachment to, and invasion of, the transfected cells are increased if the parasites are incubated with polyclonal or monoclonal antibodies against T. cruzi surface membrane antigens other than Ssp-3. If, however, antibodies to Ssp-3 are added to the incubation mixtures containing any of the other anti-T. cruzi antibodies, the enhancement of invasion (but not of attachment) is reversed. These results suggest that Ssp-3-bearing molecules participate in the process of parasite internalization. |
publishDate |
1992 |
dc.date.issued.fl_str_mv |
1992-06-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T11:40:06Z |
dc.date.available.fl_str_mv |
2016-01-24T11:40:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Experimental Medicine. New York: Rockefeller Univ Press, v. 175, n. 6, p. 1635-1641, 1992. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/25252 http://dx.doi.org/10.1084/jem.175.6.1635 |
dc.identifier.issn.none.fl_str_mv |
0022-1007 |
dc.identifier.file.none.fl_str_mv |
WOSA1992HV67600025.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1084/jem.175.6.1635 |
dc.identifier.wos.none.fl_str_mv |
WOS:A1992HV67600025 |
identifier_str_mv |
Journal of Experimental Medicine. New York: Rockefeller Univ Press, v. 175, n. 6, p. 1635-1641, 1992. 0022-1007 WOSA1992HV67600025.pdf 10.1084/jem.175.6.1635 WOS:A1992HV67600025 |
url |
http://repositorio.unifesp.br/handle/11600/25252 http://dx.doi.org/10.1084/jem.175.6.1635 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Experimental Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1635-1641 |
dc.publisher.none.fl_str_mv |
Rockefeller Univ Press |
publisher.none.fl_str_mv |
Rockefeller Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/25252/1/WOSA1992HV67600025.pdf ${dspace.ui.url}/bitstream/11600/25252/2/WOSA1992HV67600025.pdf.txt |
bitstream.checksum.fl_str_mv |
2ec2e73f2e4f9daeafb3c58f80e674e0 2028237dc40148890460e58c2b40d0dc |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764112435871744 |