Modulação de processos inflamatórios in vivo sob ação do laser de baixa intensidade em camundongos: citocinas pró e anti-inflamatórias

• Autor(a) principal: Fukuda, Thiago Yukio [UNIFESP]
• Data de Publicação: 2011
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/9823
• topic: Inflamação; Interleucinas; Terapia a laser de baixa intensidade; Citocinas

Purpose: To evaluate the modulation of pro-inflammatory (interleukin-6, IL-6; tumor necrosis factor-, TNF-; and interferon-, IFN-) and anti-inflammatory cytokines (transforming growth factor-1, TGF-1) in the inflammation processes in vivo with lowlevel laser action. Methods: Fifty isogenic mice were randomly distributed in three groups: control (no surgical procedure, n = 10), sham (surgical procedure with three standard cutaneous incisions, followed by an abdominal muscle incision and suture, n = 20) and laser (same procedure followed by laser exposure, n = 20). The sham group was divided into three subgroups: sham I (euthanasia and evaluation, 36 hours after surgical procedure), sham II (euthanasia and evaluation, 60 hours after surgical procedure), and sham III (euthanasia and evaluation, 84 hours after surgical procedure). The laser group was also divided in three subgroups: laser I (a single laser session, 12 hours after surgery), laser II (two laser sessions, 12 and 36 hours after surgery), and laser III (three laser sessions, 12, 36 and 60 hours after surgery). All animals in the laser group received three points per session of continuous infrared laser (wavelength of 780 nm, power of 20 mW, fluency of 10 Jcm2, exposure time of 20 seconds per point, and energy of 0.4 J). After euthanasia, spleen mononuclear cells were isolated and cultured for 48 hours. Concentrations of IL-6, TNF-, IFN- and TGF-1 were obtained by enzyme-linked immunosorbent assay method (ELISA). Results: There was significant difference of the IL-6 and TNF- concentrations in the 60 and 84 hour evaluations when the laser and sham groups were compared to the control group (p < 0.05), except for laser II in the TNF- analysis (p > 0.05). The IFN- concentration analysis showed a significant difference only in sham II when compared to the control group (p < 0.05). Thus, there was a modulatory effect of the TNF- and IFN- in the laser group, particularly in the 60-hour postoperative evaluation. There was no significant difference between the laser, sham, and control groups for TGF-ß1 analysis (p > 0.05). Conclusion: The low-level laser showed a modulatory effect in vivo of pro-inflammatory cytokines, especially after two exposure sessions by decreasing the TNF- and IFN- release of spleen mononuclear cells in mice. However, there was no modulation of the IL-6 and TGF-1 release.