H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1186/s40345-017-0088-2 https://repositorio.unifesp.br/handle/11600/53670 |
Resumo: | Background: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods: Metabolomic profiling, employing H-1-NMR, H-1-NMR -T-2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-L-phenyl alanine, N-acetyl-L-aspartyl-L-glutamic acid, L-glutamine). In addition, amygdalin, alpha-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up. |
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Sethi, Sumit [UNIFESP]Pedrini, Mariana [UNIFESP]Rizzo, Lucas B. [UNIFESP]Zeni-Graiff, Maiara [UNIFESP]Dal Mas, Caroline [UNIFESP]Cassinelli, Ana ClaudiaNoto, Mariane N. [UNIFESP]Asevedo, Elson [UNIFESP]Cordeiro, QuirinoPontes, Joao G. M.Brasil, Antonio J. M.Lacerda, Acioly [UNIFESP]Hayashi, Mirian A. F. [UNIFESP]Poppi, RoneiTasic, LjubicaBrietzke, Elisa [UNIFESP]2020-06-26T16:30:37Z2020-06-26T16:30:37Z2017http://dx.doi.org/10.1186/s40345-017-0088-2International Journal Of Bipolar Disorders. Heidelberg, v. 5, p. -, 2017.2194-7511https://repositorio.unifesp.br/handle/11600/53670WOS000403186100001.pdf10.1186/s40345-017-0088-2WOS:000403186100001Background: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods: Metabolomic profiling, employing H-1-NMR, H-1-NMR -T-2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-L-phenyl alanine, N-acetyl-L-aspartyl-L-glutamic acid, L-glutamine). In addition, amygdalin, alpha-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up.Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brasilia, Brazil)FAPESPUniv Fed Sao Paulo UNIFESP, Dept Psychiat, Rua Borges Lagoa 570, BR-04038020 Sao Paulo, BrazilUniv Fed Sao Paulo UNIFESP, Dept Pharmacol, Rua Tres Maio 100, BR-04044020 Sao Paulo, BrazilISCMSP, Dept Psychiat, Rua Major Maragliano 287, BR-04017030 Sao Paulo, BrazilUniv Estadual Campinas UNICAMP, Lab Quim Biol, Dept Organ Chem, Inst Chem, Caixa Postal 6154, BR-13083970 Sao Paulo, BrazilUniv Estadual Campinas UNICAMP, Dept Analyt Chem, Inst Chem, Caixa Postal 6154, BR-13083970 Sao Paulo, BrazilUniv Fed Sao Paulo UNIFESP, Dept Psychiat, Rua Borges Lagoa 570, BR-04038020 Sao Paulo, BrazilUniv Fed Sao Paulo UNIFESP, Dept Pharmacol, Rua Tres Maio 100, BR-04044020 Sao Paulo, BrazilCNPqFAPESP: 2014/18938-8Web of Science-engSpringer HeidelbergInternational Journal Of Bipolar DisordersH-1-NMRBiomarkersBipolar disorderMetabolic profilingChemometrics2D NMRH-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profilinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHeidelberg5info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000403186100001.pdfapplication/pdf1277682${dspace.ui.url}/bitstream/11600/53670/1/WOS000403186100001.pdffe430d3822f34017d339048bf3348f9dMD51open accessTEXTWOS000403186100001.pdf.txtWOS000403186100001.pdf.txtExtracted texttext/plain33443${dspace.ui.url}/bitstream/11600/53670/2/WOS000403186100001.pdf.txtc1ecb5864801a182dacc21041f20a45eMD52open accessTHUMBNAILWOS000403186100001.pdf.jpgWOS000403186100001.pdf.jpgIM Thumbnailimage/jpeg7117${dspace.ui.url}/bitstream/11600/53670/4/WOS000403186100001.pdf.jpg5baa0fa888d1ba23968d078f6e4c591aMD54open access11600/536702022-08-01 09:44:17.539open accessoai:repositorio.unifesp.br:11600/53670Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T12:44:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
title |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
spellingShingle |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling Sethi, Sumit [UNIFESP] H-1-NMR Biomarkers Bipolar disorder Metabolic profiling Chemometrics 2D NMR |
title_short |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
title_full |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
title_fullStr |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
title_full_unstemmed |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
title_sort |
H-1-NMR, H-1-NMR T-2-edited, and 2D-NMR in bipolar disorder metabolic profiling |
author |
Sethi, Sumit [UNIFESP] |
author_facet |
Sethi, Sumit [UNIFESP] Pedrini, Mariana [UNIFESP] Rizzo, Lucas B. [UNIFESP] Zeni-Graiff, Maiara [UNIFESP] Dal Mas, Caroline [UNIFESP] Cassinelli, Ana Claudia Noto, Mariane N. [UNIFESP] Asevedo, Elson [UNIFESP] Cordeiro, Quirino Pontes, Joao G. M. Brasil, Antonio J. M. Lacerda, Acioly [UNIFESP] Hayashi, Mirian A. F. [UNIFESP] Poppi, Ronei Tasic, Ljubica Brietzke, Elisa [UNIFESP] |
author_role |
author |
author2 |
Pedrini, Mariana [UNIFESP] Rizzo, Lucas B. [UNIFESP] Zeni-Graiff, Maiara [UNIFESP] Dal Mas, Caroline [UNIFESP] Cassinelli, Ana Claudia Noto, Mariane N. [UNIFESP] Asevedo, Elson [UNIFESP] Cordeiro, Quirino Pontes, Joao G. M. Brasil, Antonio J. M. Lacerda, Acioly [UNIFESP] Hayashi, Mirian A. F. [UNIFESP] Poppi, Ronei Tasic, Ljubica Brietzke, Elisa [UNIFESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sethi, Sumit [UNIFESP] Pedrini, Mariana [UNIFESP] Rizzo, Lucas B. [UNIFESP] Zeni-Graiff, Maiara [UNIFESP] Dal Mas, Caroline [UNIFESP] Cassinelli, Ana Claudia Noto, Mariane N. [UNIFESP] Asevedo, Elson [UNIFESP] Cordeiro, Quirino Pontes, Joao G. M. Brasil, Antonio J. M. Lacerda, Acioly [UNIFESP] Hayashi, Mirian A. F. [UNIFESP] Poppi, Ronei Tasic, Ljubica Brietzke, Elisa [UNIFESP] |
dc.subject.eng.fl_str_mv |
H-1-NMR Biomarkers Bipolar disorder Metabolic profiling Chemometrics 2D NMR |
topic |
H-1-NMR Biomarkers Bipolar disorder Metabolic profiling Chemometrics 2D NMR |
description |
Background: The objective of this study was to identify molecular alterations in the human blood serum related to bipolar disorder, using nuclear magnetic resonance (NMR) spectroscopy and chemometrics. Methods: Metabolomic profiling, employing H-1-NMR, H-1-NMR -T-2-edited, and 2D-NMR spectroscopy and chemometrics of human blood serum samples from patients with bipolar disorder (n = 26) compared with healthy volunteers (n = 50) was performed. Results: The investigated groups presented distinct metabolic profiles, in which the main differential metabolites found in the serum sample of bipolar disorder patients compared with those from controls were lipids, lipid metabolism-related molecules (choline, myo-inositol), and some amino acids (N-acetyl-L-phenyl alanine, N-acetyl-L-aspartyl-L-glutamic acid, L-glutamine). In addition, amygdalin, alpha-ketoglutaric acid, and lipoamide, among other compounds, were also present or were significantly altered in the serum of bipolar disorder patients. The data presented herein suggest that some of these metabolites differentially distributed between the groups studied may be directly related to the bipolar disorder pathophysiology. Conclusions: The strategy employed here showed significant potential for exploring pathophysiological features and molecular pathways involved in bipolar disorder. Thus, our findings may contribute to pave the way for future studies aiming at identifying important potential biomarkers for bipolar disorder diagnosis or progression follow-up. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-06-26T16:30:37Z |
dc.date.available.fl_str_mv |
2020-06-26T16:30:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.].fl_str_mv |
http://dx.doi.org/10.1186/s40345-017-0088-2 |
dc.identifier.citation.fl_str_mv |
International Journal Of Bipolar Disorders. Heidelberg, v. 5, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/53670 |
dc.identifier.issn.none.fl_str_mv |
2194-7511 |
dc.identifier.file.none.fl_str_mv |
WOS000403186100001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/s40345-017-0088-2 |
dc.identifier.wos.none.fl_str_mv |
WOS:000403186100001 |
url |
http://dx.doi.org/10.1186/s40345-017-0088-2 https://repositorio.unifesp.br/handle/11600/53670 |
identifier_str_mv |
International Journal Of Bipolar Disorders. Heidelberg, v. 5, p. -, 2017. 2194-7511 WOS000403186100001.pdf 10.1186/s40345-017-0088-2 WOS:000403186100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
International Journal Of Bipolar Disorders |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- |
dc.coverage.none.fl_str_mv |
Heidelberg |
dc.publisher.none.fl_str_mv |
Springer Heidelberg |
publisher.none.fl_str_mv |
Springer Heidelberg |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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