Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38458 http://dx.doi.org/10.1371/journal.pone.0112748 |
Resumo: | Background and Purpose: Leaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. the present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental Approach: Phenolic compounds, flavonoids and saponins were evaluated in an ethanol: water (80: 20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). the antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key Results: Phenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3 +/- 3.3 mu g/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.Conclusions: E-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda. |
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Casagrande, Junior CesarBenitez Macorini, Luis FernandoAntunes, Katia AvilaSantos, Uilson Pereira dosCampos, Jaqueline FerreiraDias-Junior, Nelson MiguelSangalli, AndreiaLima Cardoso, Claudia AndreaVieira, Maria do CarmoRabelo, Luiza AntasParedes-Gamero, Edgar Julian [UNIFESP]Santos, Edson Lucas dosSouza, Kely de PicoliFed Univ Grande DouradosUniversidade Estadual de Mato Grosso do Sul (UEMS)Univ Fed AlagoasUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:38:11Z2016-01-24T14:38:11Z2014-11-17Plos One. San Francisco: Public Library Science, v. 9, n. 11, 8 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/38458http://dx.doi.org/10.1371/journal.pone.0112748WOS000345158700067.pdf10.1371/journal.pone.0112748WOS:000345158700067Background and Purpose: Leaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. the present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental Approach: Phenolic compounds, flavonoids and saponins were evaluated in an ethanol: water (80: 20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). the antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key Results: Phenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3 +/- 3.3 mu g/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.Conclusions: E-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda.Fundacao de Apoio ao Desenvolvimento do EnsinoCiencia e Tecnologia do Mato Grosso do Sul (FUNDECT)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fed Univ Grande Dourados, Dourados, MS, BrazilUniv Estadual Mato Grosso do Sul, Dourados, MS, BrazilUniv Fed Alagoas, Maceio, Alagoas, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, EPM, São Paulo, BrazilWeb of Science8engPublic Library SciencePlos OneAntioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leavesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000345158700067.pdfapplication/pdf627515${dspace.ui.url}/bitstream/11600/38458/1/WOS000345158700067.pdfa416db9cb869d82dc00c779694708906MD51open accessTEXTWOS000345158700067.pdf.txtWOS000345158700067.pdf.txtExtracted texttext/plain42943${dspace.ui.url}/bitstream/11600/38458/2/WOS000345158700067.pdf.txt24f91463d873f758118e953c85a39659MD52open access11600/384582022-06-02 09:35:00.224open accessoai:repositorio.unifesp.br:11600/38458Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-06-02T12:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
title |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
spellingShingle |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves Casagrande, Junior Cesar |
title_short |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
title_full |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
title_fullStr |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
title_full_unstemmed |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
title_sort |
Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves |
author |
Casagrande, Junior Cesar |
author_facet |
Casagrande, Junior Cesar Benitez Macorini, Luis Fernando Antunes, Katia Avila Santos, Uilson Pereira dos Campos, Jaqueline Ferreira Dias-Junior, Nelson Miguel Sangalli, Andreia Lima Cardoso, Claudia Andrea Vieira, Maria do Carmo Rabelo, Luiza Antas Paredes-Gamero, Edgar Julian [UNIFESP] Santos, Edson Lucas dos Souza, Kely de Picoli |
author_role |
author |
author2 |
Benitez Macorini, Luis Fernando Antunes, Katia Avila Santos, Uilson Pereira dos Campos, Jaqueline Ferreira Dias-Junior, Nelson Miguel Sangalli, Andreia Lima Cardoso, Claudia Andrea Vieira, Maria do Carmo Rabelo, Luiza Antas Paredes-Gamero, Edgar Julian [UNIFESP] Santos, Edson Lucas dos Souza, Kely de Picoli |
author2_role |
author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Fed Univ Grande Dourados Universidade Estadual de Mato Grosso do Sul (UEMS) Univ Fed Alagoas Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Casagrande, Junior Cesar Benitez Macorini, Luis Fernando Antunes, Katia Avila Santos, Uilson Pereira dos Campos, Jaqueline Ferreira Dias-Junior, Nelson Miguel Sangalli, Andreia Lima Cardoso, Claudia Andrea Vieira, Maria do Carmo Rabelo, Luiza Antas Paredes-Gamero, Edgar Julian [UNIFESP] Santos, Edson Lucas dos Souza, Kely de Picoli |
description |
Background and Purpose: Leaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. the present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental Approach: Phenolic compounds, flavonoids and saponins were evaluated in an ethanol: water (80: 20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). the antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key Results: Phenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3 +/- 3.3 mu g/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.Conclusions: E-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-17 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:11Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 11, 8 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38458 http://dx.doi.org/10.1371/journal.pone.0112748 |
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1932-6203 |
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WOS000345158700067.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0112748 |
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WOS:000345158700067 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 11, 8 p., 2014. 1932-6203 WOS000345158700067.pdf 10.1371/journal.pone.0112748 WOS:000345158700067 |
url |
http://repositorio.unifesp.br/handle/11600/38458 http://dx.doi.org/10.1371/journal.pone.0112748 |
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eng |
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Public Library Science |
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