Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine

Detalhes bibliográficos
Autor(a) principal: Borges, Antonio Carlos Romao [UNIFESP]
Data de Publicação: 1999
Outros Autores: Feres, Teresa [UNIFESP], Vianna, L. M., Paiva, Therezinha Bandiera [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://doi.org/10.1161/01.HYP.34.4.897
http://repositorio.unifesp.br/handle/11600/43887
Resumo: We studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide.
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spelling Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholinerats, inbred SHRarteriesmembranespotassiumacetylcholinecholecalciferolWe studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide.Escola Paulista Med, Dept Biophys, BR-04023062 Sao Paulo, BrazilEscola Paulista Med, Dept Biophys, BR-04023062 Sao Paulo, BrazilWeb of ScienceLippincott Williams & WilkinsUniversidade Federal de São Paulo (UNIFESP)Borges, Antonio Carlos Romao [UNIFESP]Feres, Teresa [UNIFESP]Vianna, L. M.Paiva, Therezinha Bandiera [UNIFESP]2018-06-15T17:38:26Z2018-06-15T17:38:26Z1999-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion897-901https://doi.org/10.1161/01.HYP.34.4.897Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999.10.1161/01.HYP.34.4.8970194-911Xhttp://repositorio.unifesp.br/handle/11600/43887WOS:000083486500035engHypertensioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:44:18Zoai:repositorio.unifesp.br/:11600/43887Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:44:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
title Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
spellingShingle Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
Borges, Antonio Carlos Romao [UNIFESP]
rats, inbred SHR
arteries
membranes
potassium
acetylcholine
cholecalciferol
title_short Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
title_full Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
title_fullStr Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
title_full_unstemmed Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
title_sort Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
author Borges, Antonio Carlos Romao [UNIFESP]
author_facet Borges, Antonio Carlos Romao [UNIFESP]
Feres, Teresa [UNIFESP]
Vianna, L. M.
Paiva, Therezinha Bandiera [UNIFESP]
author_role author
author2 Feres, Teresa [UNIFESP]
Vianna, L. M.
Paiva, Therezinha Bandiera [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Borges, Antonio Carlos Romao [UNIFESP]
Feres, Teresa [UNIFESP]
Vianna, L. M.
Paiva, Therezinha Bandiera [UNIFESP]
dc.subject.por.fl_str_mv rats, inbred SHR
arteries
membranes
potassium
acetylcholine
cholecalciferol
topic rats, inbred SHR
arteries
membranes
potassium
acetylcholine
cholecalciferol
description We studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide.
publishDate 1999
dc.date.none.fl_str_mv 1999-10-01
2018-06-15T17:38:26Z
2018-06-15T17:38:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1161/01.HYP.34.4.897
Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999.
10.1161/01.HYP.34.4.897
0194-911X
http://repositorio.unifesp.br/handle/11600/43887
WOS:000083486500035
url https://doi.org/10.1161/01.HYP.34.4.897
http://repositorio.unifesp.br/handle/11600/43887
identifier_str_mv Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999.
10.1161/01.HYP.34.4.897
0194-911X
WOS:000083486500035
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Hypertension
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 897-901
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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