Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://doi.org/10.1161/01.HYP.34.4.897 http://repositorio.unifesp.br/handle/11600/43887 |
Resumo: | We studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide. |
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Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholinerats, inbred SHRarteriesmembranespotassiumacetylcholinecholecalciferolWe studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide.Escola Paulista Med, Dept Biophys, BR-04023062 Sao Paulo, BrazilEscola Paulista Med, Dept Biophys, BR-04023062 Sao Paulo, BrazilWeb of ScienceLippincott Williams & WilkinsUniversidade Federal de São Paulo (UNIFESP)Borges, Antonio Carlos Romao [UNIFESP]Feres, Teresa [UNIFESP]Vianna, L. M.Paiva, Therezinha Bandiera [UNIFESP]2018-06-15T17:38:26Z2018-06-15T17:38:26Z1999-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion897-901https://doi.org/10.1161/01.HYP.34.4.897Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999.10.1161/01.HYP.34.4.8970194-911Xhttp://repositorio.unifesp.br/handle/11600/43887WOS:000083486500035engHypertensioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:44:18Zoai:repositorio.unifesp.br/:11600/43887Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:44:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
title |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
spellingShingle |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine Borges, Antonio Carlos Romao [UNIFESP] rats, inbred SHR arteries membranes potassium acetylcholine cholecalciferol |
title_short |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
title_full |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
title_fullStr |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
title_full_unstemmed |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
title_sort |
Effect of cholecalciferol treatment on the relaxant responses of spontaneously hypertensive rat arteries to acetylcholine |
author |
Borges, Antonio Carlos Romao [UNIFESP] |
author_facet |
Borges, Antonio Carlos Romao [UNIFESP] Feres, Teresa [UNIFESP] Vianna, L. M. Paiva, Therezinha Bandiera [UNIFESP] |
author_role |
author |
author2 |
Feres, Teresa [UNIFESP] Vianna, L. M. Paiva, Therezinha Bandiera [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Borges, Antonio Carlos Romao [UNIFESP] Feres, Teresa [UNIFESP] Vianna, L. M. Paiva, Therezinha Bandiera [UNIFESP] |
dc.subject.por.fl_str_mv |
rats, inbred SHR arteries membranes potassium acetylcholine cholecalciferol |
topic |
rats, inbred SHR arteries membranes potassium acetylcholine cholecalciferol |
description |
We studied the effect of oral cholecalciferol treatment on the endothelium-dependent vascular relaxation and hyperpolarization induced by acetylcholine (ACh), which is impaired in spontaneously hypertensive rats (SHR). Adult female SHR and normotensive Wistar-Kyoto rat (WKY) controls received 125 mu g of cholecalciferol per kilogram body weight per day for 6 weeks. The responses to ACh of the isolated mesenteric vascular bed and mesenteric artery rings were measured, as well as the smooth muscle cell membrane potential. After cholecalciferol treatment, the systolic blood pressure and basal perfusion pressure of the mesenteric vascular bed of the SHR fell to control levels. The relaxant and hyperpolarizing effects of ACh, which are reduced in SHR, were also brought to control levels after cholecalciferol treatment. These effects of ACh were inhibited by N-omega-nitro-L-arginine in SHR and by apamin in WKY. After cholecalciferol treatment, SHR hyperpolarizing responses showed the same inhibition pattern as those of WKY. This indicates that, after cholecalciferol treatment, SHR vascular mesenteric preparation responses to ACh are mediated by endothelium-derived hyperpolarizing factor, which induces activation of Ca2+-dependent K+ channels, as in WKY. In untreated SHR, the ACh-mediated response is entirely due to ACh acting via the release of nitric oxide. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-10-01 2018-06-15T17:38:26Z 2018-06-15T17:38:26Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1161/01.HYP.34.4.897 Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999. 10.1161/01.HYP.34.4.897 0194-911X http://repositorio.unifesp.br/handle/11600/43887 WOS:000083486500035 |
url |
https://doi.org/10.1161/01.HYP.34.4.897 http://repositorio.unifesp.br/handle/11600/43887 |
identifier_str_mv |
Hypertension. Philadelphia: Lippincott Williams & Wilkins, v. 34, n. 4, p. 897-901, 1999. 10.1161/01.HYP.34.4.897 0194-911X WOS:000083486500035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hypertension |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
897-901 |
dc.publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1824718342205734912 |