Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats

Detalhes bibliográficos
Autor(a) principal: Silva, A. V. da
Data de Publicação: 2005
Outros Autores: Regondi, M. C., Cipelletti, B., Frassoni, C., Cavalheiro, Esper Abrão [UNIFESP], Spreafico, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000008v2r
Texto Completo: https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x
http://repositorio.unifesp.br/handle/11600/43553
Resumo: Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life.
id UFSP_b29ddc2e2b0a093a2e518e6ab4c190e7
oai_identifier_str oai:repositorio.unifesp.br/:11600/43553
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in ratsimmature brainearly insultimmunocytochemistryimmunohistoblotPurpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life.UNIFESP EPM, Lab Neurol Expt, BR-04023900 Sao Paulo, BrazilIst Nazl Neurol Carlo Besta, Milan, ItalyUNIFESP EPM, Lab Neurol Expt, BR-04023900 Sao Paulo, BrazilWeb of ScienceWiley-BlackwellUniversidade Federal de São Paulo (UNIFESP)Ist Nazl Neurol Carlo BestaSilva, A. V. daRegondi, M. C.Cipelletti, B.Frassoni, C.Cavalheiro, Esper Abrão [UNIFESP]Spreafico, R.2018-06-15T17:17:31Z2018-06-15T17:17:31Z2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion636-642https://dx.doi.org/10.1111/j.1528-1167.2005.31604.xEpilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005.10.1111/j.1528-1167.2005.31604.x0013-9580http://repositorio.unifesp.br/handle/11600/43553WOS:000228560000004ark:/48912/0013000008v2rengEpilepsiainfo:eu-repo/semantics/openAccesshttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:57:06Zoai:repositorio.unifesp.br/:11600/43553Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:05:46.661196Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
title Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
spellingShingle Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
Silva, A. V. da
immature brain
early insult
immunocytochemistry
immunohistoblot
title_short Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
title_full Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
title_fullStr Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
title_full_unstemmed Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
title_sort Neocortical and hippocampal changes after multiple pilocarpine-induced status epilepticus in rats
author Silva, A. V. da
author_facet Silva, A. V. da
Regondi, M. C.
Cipelletti, B.
Frassoni, C.
Cavalheiro, Esper Abrão [UNIFESP]
Spreafico, R.
author_role author
author2 Regondi, M. C.
Cipelletti, B.
Frassoni, C.
Cavalheiro, Esper Abrão [UNIFESP]
Spreafico, R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Ist Nazl Neurol Carlo Besta
dc.contributor.author.fl_str_mv Silva, A. V. da
Regondi, M. C.
Cipelletti, B.
Frassoni, C.
Cavalheiro, Esper Abrão [UNIFESP]
Spreafico, R.
dc.subject.por.fl_str_mv immature brain
early insult
immunocytochemistry
immunohistoblot
topic immature brain
early insult
immunocytochemistry
immunohistoblot
description Purpose: Multiple episodes of pilocarpine-induced status epilepticus (SE) in developing rats (P7-P9) lead to progressive epileptiform activity and severe cognitive impairment in adulthood. The present work studied possible underlying abnormalities in the neocortex and hippocampus of pilocarpine-treated animals.Methods: Wistar rats were submitted to pilocarpine-induced SE at P7, P8, and P9, and were killed at P35. Immunocytochemistry was performed on 50-mu m vibratome sections, by using antibodies against nonphosphorylated neurofilament (SMI-311), parvalbumin (PV), calbindin (CB), calretinin (CR), and glutamate decarboxylase (GAD-65). Ten-micron cryostat sections were processed for immunohistoblot by using antibodies against GluR1, GluR2/3, and GluR4 alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits and NR2ab N-methyl-D-aspartate (NMDA) receptor subunit.Results: Adult rats submitted to SE at P7-9 showed: (a) altered distribution of neocortical interneurons; (b) increased cortical and reduced hippocampal GAD-65 expression; and (c) altered expression of hippocampal AMPA and NMDA receptors.Conclusions: We conclude that multiple SE episodes during P7-9 generate long-lasting disturbances that underlie behavioral and electrographic abnormalities later in life.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
2018-06-15T17:17:31Z
2018-06-15T17:17:31Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x
Epilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005.
10.1111/j.1528-1167.2005.31604.x
0013-9580
http://repositorio.unifesp.br/handle/11600/43553
WOS:000228560000004
dc.identifier.dark.fl_str_mv ark:/48912/0013000008v2r
url https://dx.doi.org/10.1111/j.1528-1167.2005.31604.x
http://repositorio.unifesp.br/handle/11600/43553
identifier_str_mv Epilepsia. Hoboken: Wiley-blackwell, v. 46, n. 5, p. 636-642, 2005.
10.1111/j.1528-1167.2005.31604.x
0013-9580
WOS:000228560000004
ark:/48912/0013000008v2r
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Epilepsia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://olabout.wiley.com/WileyCDA/Section/id-406071.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.format.none.fl_str_mv 636-642
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602428203794432