Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/56262 http://dx.doi.org/10.1590/S1678-9946201759014 |
Resumo: | The aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species. |
id |
UFSP_b49c9809aa5961182c326bd2718d259f |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/56262 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Liverio, Harisson OliveiraRuiz, Luciana da Silvade Freitas, Roseli SantosNishikaku, Angela Satie [UNIFESP]de Souza, Ana ClaraPaula, Claudete RodriguesDomaneschi, Carina2020-07-31T12:46:31Z2020-07-31T12:46:31Z2017Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017.0036-4665https://repositorio.unifesp.br/handle/11600/56262http://dx.doi.org/10.1590/S1678-9946201759014S0036-46652017005000209.pdfS0036-4665201700500020910.1590/S1678-9946201759014WOS:000404610000003The aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Univ Sao Paulo, Fac Odontol, Dept Estomatol, Ave Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP, BrazilAdolfo Lutz Inst, Nucleo Ciencias Biomed, Sao Paulo, BrazilUniv Sao Paulo, Inst Med Trop Sao Paulo, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Lab Especial Micol, Sao Paulo, SP, BrazilWeb of Science-engInst Medicina Tropical Sao PauloRevista Do Instituto De Medicina Tropical De Sao PauloCandida dubliniensisCandida albicansChildrenHIV/AIDS Phenotypic and genotypic methodsPhenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSao Paulo59info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0036-46652017005000209.pdfapplication/pdf188570${dspace.ui.url}/bitstream/11600/56262/1/S0036-46652017005000209.pdff9d5510ec1d5418e46e335a3fbc1aab7MD51open accessTEXTS0036-46652017005000209.pdf.txtS0036-46652017005000209.pdf.txtExtracted texttext/plain27716${dspace.ui.url}/bitstream/11600/56262/2/S0036-46652017005000209.pdf.txte77dcc91eb0c2a87a7872eaed3bb87dbMD52open accessTHUMBNAILS0036-46652017005000209.pdf.jpgS0036-46652017005000209.pdf.jpgIM Thumbnailimage/jpeg7042${dspace.ui.url}/bitstream/11600/56262/4/S0036-46652017005000209.pdf.jpg98aec7da09e0c9dc4d4b6bf86e0c579bMD54open access11600/562622022-08-01 00:43:49.583open accessoai:repositorio.unifesp.br:11600/56262Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T03:43:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
title |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
spellingShingle |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients Liverio, Harisson Oliveira Candida dubliniensis Candida albicans Children HIV/AIDS Phenotypic and genotypic methods |
title_short |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
title_full |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
title_fullStr |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
title_full_unstemmed |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
title_sort |
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients |
author |
Liverio, Harisson Oliveira |
author_facet |
Liverio, Harisson Oliveira Ruiz, Luciana da Silva de Freitas, Roseli Santos Nishikaku, Angela Satie [UNIFESP] de Souza, Ana Clara Paula, Claudete Rodrigues Domaneschi, Carina |
author_role |
author |
author2 |
Ruiz, Luciana da Silva de Freitas, Roseli Santos Nishikaku, Angela Satie [UNIFESP] de Souza, Ana Clara Paula, Claudete Rodrigues Domaneschi, Carina |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Liverio, Harisson Oliveira Ruiz, Luciana da Silva de Freitas, Roseli Santos Nishikaku, Angela Satie [UNIFESP] de Souza, Ana Clara Paula, Claudete Rodrigues Domaneschi, Carina |
dc.subject.eng.fl_str_mv |
Candida dubliniensis Candida albicans Children HIV/AIDS Phenotypic and genotypic methods |
topic |
Candida dubliniensis Candida albicans Children HIV/AIDS Phenotypic and genotypic methods |
description |
The aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2020-07-31T12:46:31Z |
dc.date.available.fl_str_mv |
2020-07-31T12:46:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/56262 http://dx.doi.org/10.1590/S1678-9946201759014 |
dc.identifier.issn.none.fl_str_mv |
0036-4665 |
dc.identifier.file.none.fl_str_mv |
S0036-46652017005000209.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0036-46652017005000209 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1678-9946201759014 |
dc.identifier.wos.none.fl_str_mv |
WOS:000404610000003 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017. 0036-4665 S0036-46652017005000209.pdf S0036-46652017005000209 10.1590/S1678-9946201759014 WOS:000404610000003 |
url |
https://repositorio.unifesp.br/handle/11600/56262 http://dx.doi.org/10.1590/S1678-9946201759014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- |
dc.coverage.none.fl_str_mv |
Sao Paulo |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/56262/1/S0036-46652017005000209.pdf ${dspace.ui.url}/bitstream/11600/56262/2/S0036-46652017005000209.pdf.txt ${dspace.ui.url}/bitstream/11600/56262/4/S0036-46652017005000209.pdf.jpg |
bitstream.checksum.fl_str_mv |
f9d5510ec1d5418e46e335a3fbc1aab7 e77dcc91eb0c2a87a7872eaed3bb87db 98aec7da09e0c9dc4d4b6bf86e0c579b |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764126933483520 |