Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34795 http://dx.doi.org/10.1016/j.regpep.2012.01.003 |
Resumo: | Angiotensin II (All), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. the aim of this study was to evaluate potential signaling pathways mediated by All in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. All increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that All rapidly (3 min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein. (C) 2012 Elsevier B.V. All rights reserved. |
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Alves, Eduardo da Silva [UNIFESP]Haidar, André Abou [UNIFESP]Quadros, Caren Dal'Mora de [UNIFESP]Carvalho, Daniela S. [UNIFESP]Morgan, DanielaRocha, Marlene Santos daCuri, RuiCarpinelli, Angelo RafaelHirata, Aparecida Emiko [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2016-01-24T14:27:06Z2016-01-24T14:27:06Z2012-04-10Regulatory Peptides. Amsterdam: Elsevier B.V., v. 175, n. 1-3, p. 1-6, 2012.0167-0115http://repositorio.unifesp.br/handle/11600/34795http://dx.doi.org/10.1016/j.regpep.2012.01.003WOS000302985400001.pdf10.1016/j.regpep.2012.01.003WOS:000302985400001Angiotensin II (All), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. the aim of this study was to evaluate potential signaling pathways mediated by All in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. All increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that All rapidly (3 min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilUniv São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Fisiol, BR-04023062 São Paulo, BrazilWeb of Science1-6engElsevier B.V.Regulatory Peptideshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessAngiotensin IIIsolated isletsMAPKsJAK/STATAngiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic isletsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000302985400001.pdfapplication/pdf756253${dspace.ui.url}/bitstream/11600/34795/1/WOS000302985400001.pdf0762df49b0bfcfcd8d2b377380fe9bcfMD51open accessTEXTWOS000302985400001.pdf.txtWOS000302985400001.pdf.txtExtracted texttext/plain29831${dspace.ui.url}/bitstream/11600/34795/2/WOS000302985400001.pdf.txt56690768ef7a5f257ff8e9008c9a4cf0MD52open access11600/347952023-02-15 09:30:31.856open accessoai:repositorio.unifesp.br:11600/34795Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-02-15T12:30:31Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
title |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
spellingShingle |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets Alves, Eduardo da Silva [UNIFESP] Angiotensin II Isolated islets MAPKs JAK/STAT |
title_short |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
title_full |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
title_fullStr |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
title_full_unstemmed |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
title_sort |
Angiotensin II-induced JNK activation is mediated by NAD(P)H oxidase in isolated rat pancreatic islets |
author |
Alves, Eduardo da Silva [UNIFESP] |
author_facet |
Alves, Eduardo da Silva [UNIFESP] Haidar, André Abou [UNIFESP] Quadros, Caren Dal'Mora de [UNIFESP] Carvalho, Daniela S. [UNIFESP] Morgan, Daniela Rocha, Marlene Santos da Curi, Rui Carpinelli, Angelo Rafael Hirata, Aparecida Emiko [UNIFESP] |
author_role |
author |
author2 |
Haidar, André Abou [UNIFESP] Quadros, Caren Dal'Mora de [UNIFESP] Carvalho, Daniela S. [UNIFESP] Morgan, Daniela Rocha, Marlene Santos da Curi, Rui Carpinelli, Angelo Rafael Hirata, Aparecida Emiko [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Alves, Eduardo da Silva [UNIFESP] Haidar, André Abou [UNIFESP] Quadros, Caren Dal'Mora de [UNIFESP] Carvalho, Daniela S. [UNIFESP] Morgan, Daniela Rocha, Marlene Santos da Curi, Rui Carpinelli, Angelo Rafael Hirata, Aparecida Emiko [UNIFESP] |
dc.subject.eng.fl_str_mv |
Angiotensin II Isolated islets MAPKs JAK/STAT |
topic |
Angiotensin II Isolated islets MAPKs JAK/STAT |
description |
Angiotensin II (All), the active component of the renin angiotensin system (RAS), plays a vital role in the regulation of physiological processes of the cardiovascular system, but also has autocrine and paracrine actions in various tissues and organs. Many studies have shown the existence of RAS in the pancreas of humans and rodents. the aim of this study was to evaluate potential signaling pathways mediated by All in isolated pancreatic islets of rats. Phosphorylation of MAPKs (ERK1/2, JNK and p38MAPK), and the interaction between proteins JAK/STAT were evaluated. All increased JAK2/STAT1 (42%) and JAK2/STAT3 (100%) interaction without altering the total content of JAK2. Analyzing the activation of MAPKs (ERK1/2, JNK and p38MAPK) in isolated pancreatic islets from rats we observed that All rapidly (3 min) promoted a significant increase in the phosphorylation degree of these proteins after incubation with the hormone. Curiously JNK protein phosphorylation was inhibited by DPI, suggesting the involvement of NAD(P)H oxidase in the activation of protein. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-04-10 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:06Z |
dc.date.available.fl_str_mv |
2016-01-24T14:27:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Regulatory Peptides. Amsterdam: Elsevier B.V., v. 175, n. 1-3, p. 1-6, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34795 http://dx.doi.org/10.1016/j.regpep.2012.01.003 |
dc.identifier.issn.none.fl_str_mv |
0167-0115 |
dc.identifier.file.none.fl_str_mv |
WOS000302985400001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.regpep.2012.01.003 |
dc.identifier.wos.none.fl_str_mv |
WOS:000302985400001 |
identifier_str_mv |
Regulatory Peptides. Amsterdam: Elsevier B.V., v. 175, n. 1-3, p. 1-6, 2012. 0167-0115 WOS000302985400001.pdf 10.1016/j.regpep.2012.01.003 WOS:000302985400001 |
url |
http://repositorio.unifesp.br/handle/11600/34795 http://dx.doi.org/10.1016/j.regpep.2012.01.003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Regulatory Peptides |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-6 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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