Experimental model for low level laser therapy on ischemic random skin flap in rats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0102-86502006000400013 http://repositorio.unifesp.br/handle/11600/3198 |
Resumo: | PURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm² energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats. |
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Experimental model for low level laser therapy on ischemic random skin flap in ratsModelo experimental para laserterapia de baixa intensidade em retalho cutâneo randômico isquêmico em ratosLaser Therapy, Low-LevelSurgical FlapsNecrosisRatsTerapia a Laser de Baixa IntensidadeRetalhos CirúrgicosNecroseRatosPURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm² energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats.OBJETIVO: Propor o desenvolvimento de um modelo experimental para verificar o efeito da laserterapia de baixa intensidade na viabilidade do retalho cutâneo randômico em ratos. MÉTODOS: A amostra constituiu-se de 24 ratos, da linhagem Wistar-EPM. O retalho cutâneo randômico foi realizado com dimensões de 10x4 cm e uma barreira plástica foi interposta entre o mesmo e o leito doador. O Grupo 1 (controle), foi submetido a uma simulação de tratamento com a irradiação laser de diodo (830 nm). O Grupo 2 foi submetido à irradiação laser de diodo (830 nm). Os animais foram submetidos a terapia a laser com densidade de energia de 36 J/cm2 (72 segundos) imediatamente após a operação e nos outros quatros dias subseqüentes. A caneta do laser foi posicionada a 90 graus em contato com o retalho cutâneo em um ponto a 2,5 cm da base cranial do retalho. No sétimo dia pós-operatório foram calculadas as porcentagens da área de necrose. RESULTADOS: O Grupo 1 apresentou média da área de necrose de 48,86% e o Grupo 2 - 23,14%. Após a análise estatística, os resultados mostraram que o Grupo 2 foi mais eficaz, quando comparado ao controle (p<0,001). CONCLUSÃO: O modelo experimental mostrou-se factível para estudos dos efeitos da terapia a laser de baixa intensidade em retalho cutâneo randômico em ratos.UNIFESPUNIFESP Surgery Department Plastic Surgery DivisionUNIFESP, Surgery Department Plastic Surgery DivisionSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Prado, Rodrigo Paschoal [UNIFESP]Liebano, Richard Eloin [UNIFESP]Hochman, Bernardo [UNIFESP]Pinfildi, Carlos Eduardo [UNIFESP]Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:36:22Z2015-06-14T13:36:22Z2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion258-262application/pdfhttp://dx.doi.org/10.1590/S0102-86502006000400013Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 4, p. 258-262, 2006.10.1590/S0102-86502006000400013S0102-86502006000400013.pdf0102-8650S0102-86502006000400013http://repositorio.unifesp.br/handle/11600/3198engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T15:31:34Zoai:repositorio.unifesp.br/:11600/3198Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T15:31:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Experimental model for low level laser therapy on ischemic random skin flap in rats Modelo experimental para laserterapia de baixa intensidade em retalho cutâneo randômico isquêmico em ratos |
| title |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| spellingShingle |
Experimental model for low level laser therapy on ischemic random skin flap in rats Prado, Rodrigo Paschoal [UNIFESP] Laser Therapy, Low-Level Surgical Flaps Necrosis Rats Terapia a Laser de Baixa Intensidade Retalhos Cirúrgicos Necrose Ratos |
| title_short |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| title_full |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| title_fullStr |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| title_full_unstemmed |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| title_sort |
Experimental model for low level laser therapy on ischemic random skin flap in rats |
| author |
Prado, Rodrigo Paschoal [UNIFESP] |
| author_facet |
Prado, Rodrigo Paschoal [UNIFESP] Liebano, Richard Eloin [UNIFESP] Hochman, Bernardo [UNIFESP] Pinfildi, Carlos Eduardo [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| author_role |
author |
| author2 |
Liebano, Richard Eloin [UNIFESP] Hochman, Bernardo [UNIFESP] Pinfildi, Carlos Eduardo [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Prado, Rodrigo Paschoal [UNIFESP] Liebano, Richard Eloin [UNIFESP] Hochman, Bernardo [UNIFESP] Pinfildi, Carlos Eduardo [UNIFESP] Ferreira, Lydia Masako [UNIFESP] |
| dc.subject.por.fl_str_mv |
Laser Therapy, Low-Level Surgical Flaps Necrosis Rats Terapia a Laser de Baixa Intensidade Retalhos Cirúrgicos Necrose Ratos |
| topic |
Laser Therapy, Low-Level Surgical Flaps Necrosis Rats Terapia a Laser de Baixa Intensidade Retalhos Cirúrgicos Necrose Ratos |
| description |
PURPOSE: To develop an experimental model to be used in the study of low level Laser therapy on viability of random skin flap in rats. METHODS: The sample was 24 Wistar-EPM rats. The random skin flap measured 10 x 4 cm and a plastic sheet was interposed between the flap and donor site. Group 1 (control) underwent sham irradiation with diode laser (830 nm). Group 2 was submitted to laser irradiation with diode laser (830 nm). The animals were submitted to Laser therapy with 36 J/cm² energy density (72 seconds) immediately after the surgery and on the four subsequent days. The probe was usually held in contact with the skin flap surface on a point at 2.5 cm cranial from the flap base. On the seventh postoperative day, the percentage of necrotic area was measured and calculated. RESULTS: Group 1 reached an average necrotic area of 48.86%, Group 2 - 23.14%. After the statistic analysis, compared with the control group, Group 2 showed a statistically significant increase in survival area (p<0.001). CONCLUSION: The experimental model proved to be reliable to be used in the study of effects of low level laser therapy in random skin flap in rats. |
| publishDate |
2006 |
| dc.date.none.fl_str_mv |
2006-08-01 2015-06-14T13:36:22Z 2015-06-14T13:36:22Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502006000400013 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 4, p. 258-262, 2006. 10.1590/S0102-86502006000400013 S0102-86502006000400013.pdf 0102-8650 S0102-86502006000400013 http://repositorio.unifesp.br/handle/11600/3198 |
| url |
http://dx.doi.org/10.1590/S0102-86502006000400013 http://repositorio.unifesp.br/handle/11600/3198 |
| identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 21, n. 4, p. 258-262, 2006. 10.1590/S0102-86502006000400013 S0102-86502006000400013.pdf 0102-8650 S0102-86502006000400013 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
258-262 application/pdf |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268402436407296 |