Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/36130 http://dx.doi.org/10.4161/viru.23112 |
Resumo: | A comparative study about protein secretion, immunogenicity and virulence was performed in order to characterize and to compare eight Sporothrix schenckii sensu stricto isolates. for virulence characterization, a murine model, based on survival assay and CFU counting was used. S. brasiliensis and S. globosa, a highly virulent and a non-virulent isolates, respectively were used as external controls. Exoantigen profiles showed different secreted molecules; the 46- and 60-kDa molecules were commonly secreted by all three species. the S. schenckii s. str. isolates could be classified as non-virulent or presenting low, medium or high virulence, based on survival times after infection and recovery of viable fungi. the humoral response profiles of mice infected with S. schenckii s. str., S. globosa and S. brasiliensis were heterogeneous; five virulent isolates (S. schenckii s. str., n = 4 and S. brasiliensis, n = 1) had in common the recognition of the 60-kDa molecule by their respective antisera, suggesting that this antigen may be involved in virulence. Furthermore, the 110-kDa molecule was secreted and recognized by antisera from four virulent isolates (S. schenckii s. str., n = 3 and S. brasiliensis, n = 1), so there is a possibility that this molecule is also related to virulence. Our findings reveal different degrees of virulence in S. schenckii s. str. isolates and suggest the correlation of protein secretion and immunogenicity with virulence of S. schenckii complex. These findings provide new insights into the pathogenesis of S. schenckii s. str. and improve the knowledge about immunogenicity and protein profiles in S. schenckii complex. |
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Fernandes, Geisa Ferreira [UNIFESP]Santos, Priscila Oliveira dos [UNIFESP]Rodrigues, Anderson Messias [UNIFESP]Sasaki, Alexandre Augusto [UNIFESP]Burger, EvaCamargo, Zoilo Pires de [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Fed Alfenas2016-01-24T14:31:29Z2016-01-24T14:31:29Z2013-04-01Virulence. Austin: Landes Bioscience, v. 4, n. 3, p. 241-249, 2013.2150-5594http://repositorio.unifesp.br/handle/11600/36130http://dx.doi.org/10.4161/viru.2311210.4161/viru.23112WOS:000316948000006A comparative study about protein secretion, immunogenicity and virulence was performed in order to characterize and to compare eight Sporothrix schenckii sensu stricto isolates. for virulence characterization, a murine model, based on survival assay and CFU counting was used. S. brasiliensis and S. globosa, a highly virulent and a non-virulent isolates, respectively were used as external controls. Exoantigen profiles showed different secreted molecules; the 46- and 60-kDa molecules were commonly secreted by all three species. the S. schenckii s. str. isolates could be classified as non-virulent or presenting low, medium or high virulence, based on survival times after infection and recovery of viable fungi. the humoral response profiles of mice infected with S. schenckii s. str., S. globosa and S. brasiliensis were heterogeneous; five virulent isolates (S. schenckii s. str., n = 4 and S. brasiliensis, n = 1) had in common the recognition of the 60-kDa molecule by their respective antisera, suggesting that this antigen may be involved in virulence. Furthermore, the 110-kDa molecule was secreted and recognized by antisera from four virulent isolates (S. schenckii s. str., n = 3 and S. brasiliensis, n = 1), so there is a possibility that this molecule is also related to virulence. Our findings reveal different degrees of virulence in S. schenckii s. str. isolates and suggest the correlation of protein secretion and immunogenicity with virulence of S. schenckii complex. These findings provide new insights into the pathogenesis of S. schenckii s. str. and improve the knowledge about immunogenicity and protein profiles in S. schenckii complex.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, BrazilUniv Fed Alfenas, Dept Microbiol & Immunol, Inst Biomed Sci, Alfenas, MG, BrazilUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, Discipline Cellular Biol, São Paulo, BrazilFAPESP: 2009/54024-2Web of Science241-249engLandes BioscienceVirulenceSporothrix schenckii complexvirulencesporotrichosisantigenswestern blottingprotein secretionimmunogenicityCharacterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/361302022-02-18 12:05:32.733metadata only accessoai:repositorio.unifesp.br:11600/36130Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-02-18T15:05:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
title |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
spellingShingle |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species Fernandes, Geisa Ferreira [UNIFESP] Sporothrix schenckii complex virulence sporotrichosis antigens western blotting protein secretion immunogenicity |
title_short |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
title_full |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
title_fullStr |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
title_full_unstemmed |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
title_sort |
Characterization of virulence profile, protein secretion and immunogenicity of different Sporothrix schenckii sensu stricto isolates compared with S. globosa and S. brasiliensis species |
author |
Fernandes, Geisa Ferreira [UNIFESP] |
author_facet |
Fernandes, Geisa Ferreira [UNIFESP] Santos, Priscila Oliveira dos [UNIFESP] Rodrigues, Anderson Messias [UNIFESP] Sasaki, Alexandre Augusto [UNIFESP] Burger, Eva Camargo, Zoilo Pires de [UNIFESP] |
author_role |
author |
author2 |
Santos, Priscila Oliveira dos [UNIFESP] Rodrigues, Anderson Messias [UNIFESP] Sasaki, Alexandre Augusto [UNIFESP] Burger, Eva Camargo, Zoilo Pires de [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Fed Alfenas |
dc.contributor.author.fl_str_mv |
Fernandes, Geisa Ferreira [UNIFESP] Santos, Priscila Oliveira dos [UNIFESP] Rodrigues, Anderson Messias [UNIFESP] Sasaki, Alexandre Augusto [UNIFESP] Burger, Eva Camargo, Zoilo Pires de [UNIFESP] |
dc.subject.eng.fl_str_mv |
Sporothrix schenckii complex virulence sporotrichosis antigens western blotting protein secretion immunogenicity |
topic |
Sporothrix schenckii complex virulence sporotrichosis antigens western blotting protein secretion immunogenicity |
description |
A comparative study about protein secretion, immunogenicity and virulence was performed in order to characterize and to compare eight Sporothrix schenckii sensu stricto isolates. for virulence characterization, a murine model, based on survival assay and CFU counting was used. S. brasiliensis and S. globosa, a highly virulent and a non-virulent isolates, respectively were used as external controls. Exoantigen profiles showed different secreted molecules; the 46- and 60-kDa molecules were commonly secreted by all three species. the S. schenckii s. str. isolates could be classified as non-virulent or presenting low, medium or high virulence, based on survival times after infection and recovery of viable fungi. the humoral response profiles of mice infected with S. schenckii s. str., S. globosa and S. brasiliensis were heterogeneous; five virulent isolates (S. schenckii s. str., n = 4 and S. brasiliensis, n = 1) had in common the recognition of the 60-kDa molecule by their respective antisera, suggesting that this antigen may be involved in virulence. Furthermore, the 110-kDa molecule was secreted and recognized by antisera from four virulent isolates (S. schenckii s. str., n = 3 and S. brasiliensis, n = 1), so there is a possibility that this molecule is also related to virulence. Our findings reveal different degrees of virulence in S. schenckii s. str. isolates and suggest the correlation of protein secretion and immunogenicity with virulence of S. schenckii complex. These findings provide new insights into the pathogenesis of S. schenckii s. str. and improve the knowledge about immunogenicity and protein profiles in S. schenckii complex. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-04-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:31:29Z |
dc.date.available.fl_str_mv |
2016-01-24T14:31:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Virulence. Austin: Landes Bioscience, v. 4, n. 3, p. 241-249, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/36130 http://dx.doi.org/10.4161/viru.23112 |
dc.identifier.issn.none.fl_str_mv |
2150-5594 |
dc.identifier.doi.none.fl_str_mv |
10.4161/viru.23112 |
dc.identifier.wos.none.fl_str_mv |
WOS:000316948000006 |
identifier_str_mv |
Virulence. Austin: Landes Bioscience, v. 4, n. 3, p. 241-249, 2013. 2150-5594 10.4161/viru.23112 WOS:000316948000006 |
url |
http://repositorio.unifesp.br/handle/11600/36130 http://dx.doi.org/10.4161/viru.23112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Virulence |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
241-249 |
dc.publisher.none.fl_str_mv |
Landes Bioscience |
publisher.none.fl_str_mv |
Landes Bioscience |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764111225815040 |