Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1002/ar.a.20045 http://repositorio.unifesp.br/handle/11600/27797 |
Resumo: | Etoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc. |
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Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phasetestisetoposideapoptosisratspermatogenesisEtoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc.Universidade Federal de São Paulo, Dept Morphol, Lab Embryol, São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Electron Microscopy, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, Lab Embryol, São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Electron Microscopy, São Paulo, BrazilWeb of ScienceWiley-BlackwellUniversidade Federal de São Paulo (UNIFESP)Stumpp, Taiza [UNIFESP]Sasso-Cerri, Estela [UNIFESP]Freymüller-Haapalainen, Edna [UNIFESP]Miraglia, Sandra Maria [UNIFESP]2016-01-24T12:37:13Z2016-01-24T12:37:13Z2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion611-622http://dx.doi.org/10.1002/ar.a.20045Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004.10.1002/ar.a.200450003-276Xhttp://repositorio.unifesp.br/handle/11600/27797WOS:000222466000005engAnatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biologyinfo:eu-repo/semantics/openAccesshttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:37:13Zoai:repositorio.unifesp.br/:11600/27797Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:37:13Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
title |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
spellingShingle |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase Stumpp, Taiza [UNIFESP] testis etoposide apoptosis rat spermatogenesis |
title_short |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
title_full |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
title_fullStr |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
title_full_unstemmed |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
title_sort |
Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase |
author |
Stumpp, Taiza [UNIFESP] |
author_facet |
Stumpp, Taiza [UNIFESP] Sasso-Cerri, Estela [UNIFESP] Freymüller-Haapalainen, Edna [UNIFESP] Miraglia, Sandra Maria [UNIFESP] |
author_role |
author |
author2 |
Sasso-Cerri, Estela [UNIFESP] Freymüller-Haapalainen, Edna [UNIFESP] Miraglia, Sandra Maria [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Stumpp, Taiza [UNIFESP] Sasso-Cerri, Estela [UNIFESP] Freymüller-Haapalainen, Edna [UNIFESP] Miraglia, Sandra Maria [UNIFESP] |
dc.subject.por.fl_str_mv |
testis etoposide apoptosis rat spermatogenesis |
topic |
testis etoposide apoptosis rat spermatogenesis |
description |
Etoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-07-01 2016-01-24T12:37:13Z 2016-01-24T12:37:13Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/ar.a.20045 Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004. 10.1002/ar.a.20045 0003-276X http://repositorio.unifesp.br/handle/11600/27797 WOS:000222466000005 |
url |
http://dx.doi.org/10.1002/ar.a.20045 http://repositorio.unifesp.br/handle/11600/27797 |
identifier_str_mv |
Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004. 10.1002/ar.a.20045 0003-276X WOS:000222466000005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
dc.format.none.fl_str_mv |
611-622 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268378028703744 |