Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase

Detalhes bibliográficos
Autor(a) principal: Stumpp, Taiza [UNIFESP]
Data de Publicação: 2004
Outros Autores: Sasso-Cerri, Estela [UNIFESP], Freymüller-Haapalainen, Edna [UNIFESP], Miraglia, Sandra Maria [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1002/ar.a.20045
http://repositorio.unifesp.br/handle/11600/27797
Resumo: Etoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc.
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spelling Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phasetestisetoposideapoptosisratspermatogenesisEtoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc.Universidade Federal de São Paulo, Dept Morphol, Lab Embryol, São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Electron Microscopy, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, Lab Embryol, São Paulo, BrazilUniversidade Federal de São Paulo, Ctr Electron Microscopy, São Paulo, BrazilWeb of ScienceWiley-BlackwellUniversidade Federal de São Paulo (UNIFESP)Stumpp, Taiza [UNIFESP]Sasso-Cerri, Estela [UNIFESP]Freymüller-Haapalainen, Edna [UNIFESP]Miraglia, Sandra Maria [UNIFESP]2016-01-24T12:37:13Z2016-01-24T12:37:13Z2004-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion611-622http://dx.doi.org/10.1002/ar.a.20045Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004.10.1002/ar.a.200450003-276Xhttp://repositorio.unifesp.br/handle/11600/27797WOS:000222466000005engAnatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biologyinfo:eu-repo/semantics/openAccesshttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:37:13Zoai:repositorio.unifesp.br/:11600/27797Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:37:13Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
title Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
spellingShingle Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
Stumpp, Taiza [UNIFESP]
testis
etoposide
apoptosis
rat
spermatogenesis
title_short Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
title_full Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
title_fullStr Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
title_full_unstemmed Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
title_sort Apoptosis and testicular alterations in albino rats treated with etoposide during the prepubertal phase
author Stumpp, Taiza [UNIFESP]
author_facet Stumpp, Taiza [UNIFESP]
Sasso-Cerri, Estela [UNIFESP]
Freymüller-Haapalainen, Edna [UNIFESP]
Miraglia, Sandra Maria [UNIFESP]
author_role author
author2 Sasso-Cerri, Estela [UNIFESP]
Freymüller-Haapalainen, Edna [UNIFESP]
Miraglia, Sandra Maria [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Stumpp, Taiza [UNIFESP]
Sasso-Cerri, Estela [UNIFESP]
Freymüller-Haapalainen, Edna [UNIFESP]
Miraglia, Sandra Maria [UNIFESP]
dc.subject.por.fl_str_mv testis
etoposide
apoptosis
rat
spermatogenesis
topic testis
etoposide
apoptosis
rat
spermatogenesis
description Etoposide is a podophyllotoxin semiderivative that is used in a variety of chemotherapy treatments, including therapy for children tumors. This drug promotes the formation of a ternary DNA-topoisomerase II-etoposide complex that triggers apoptosis. the purpose of this work was to analyze the occurrence of apoptosis in the seminiferous epithelium of prepubertal, pubertal, and adult rats treated with 10, 20, and 40 mg/Kg of etoposide during the prepubertal phase, as well as the role of apoptosis in etoposide-induced testicular damage. the rat testes were fixed in Bouin's liquid, and the apoptotic cells were quantified by means of the hematoxylin and eosin (H&E) technique (all groups) and the terminal dUTP nick end labeling (TUNEL) method (prepubertal groups only). the results obtained from both the H&E and TUNEL methods showed an increased frequency of apoptosis in the seminiferous epithelium of treated animals, except for the subgroup that received the 10-mg/Kg dose and was sacrificed 12 hr after the treatment and for the etoposide-treated pubertal group, that did not show cells suggesting apoptosis during H&E analysis. the labeled cells were mainly primary spermatocytes and differentiated spermatogonia. the prepubertal rats showed an etoposide-dose-dependent diminution of differentiated spermatogonia. Etoposide treatment during the prepubertal phase increases the frequency of apoptosis in the seminiferous epithelium, and causes serious harm to male fertility. (C) 2004 Wiley-Liss, Inc.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-01
2016-01-24T12:37:13Z
2016-01-24T12:37:13Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/ar.a.20045
Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004.
10.1002/ar.a.20045
0003-276X
http://repositorio.unifesp.br/handle/11600/27797
WOS:000222466000005
url http://dx.doi.org/10.1002/ar.a.20045
http://repositorio.unifesp.br/handle/11600/27797
identifier_str_mv Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology. Hoboken: Wiley-liss, v. 279A, n. 1, p. 611-622, 2004.
10.1002/ar.a.20045
0003-276X
WOS:000222466000005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Anatomical Record Part A-discoveries in Molecular Cellular and Evolutionary Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://olabout.wiley.com/WileyCDA/Section/id-406071.html
eu_rights_str_mv openAccess
rights_invalid_str_mv http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.format.none.fl_str_mv 611-622
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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