Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment

Detalhes bibliográficos
Autor(a) principal: Saia, Flavia Talarico [UNIFESP]
Data de Publicação: 2016
Outros Autores: Souza, Theo Syrto Octavio de, Duarte, Rubens Tadeu Delgado, Pozzi, Eloisa, Fonseca, Debora, Foresti, Eugenio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://repositorio.unifesp.br/handle/11600/58646
https://doi.org/10.1007/s00449-015-1520-6
Resumo: A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system.
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spelling Saia, Flavia Talarico [UNIFESP]Souza, Theo Syrto Octavio deDuarte, Rubens Tadeu DelgadoPozzi, EloisaFonseca, DeboraForesti, Eugenio2020-11-03T14:40:37Z2020-11-03T14:40:37Z2016Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016.1615-7591https://repositorio.unifesp.br/handle/11600/58646https://doi.org/10.1007/s00449-015-1520-610.1007/s00449-015-1520-6WOS:000368723100014A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Paulo, Dept Sea Sci, Av Saldanha Da Gama 89, BR-11030400 Santos, SP, BrazilUniv Sao Paulo, Dept Hydraul & Environm Engn, Polytech Sch, Av Prof Almeida Prado 83,Trav 2,Cidade Univ, BR-05508900 Sao Paulo, SP, BrazilUniv Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, Florianopolis, SC, BrazilUniv Sao Paulo, Sch Engn Sao Carlos, Lab Biol Proc, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, BrazilUniv Fed Sao Paulo, Dept Sea Sci, Av Saldanha Da Gama 89, BR-11030400 Santos, SP, BrazilFAPESP: 2007/58659-7FAPESP: 2010/03286-4FAPESP: 2010/01735-6Web of Science341-352engSpringerBioprocess And Biosystems EngineeringPilot scale reactorSewage treatmentDenitrificationSulfideMicrobial communityPyrosequencingMicrobial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew York392info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/586462023-07-31 19:36:35.963metadata only accessoai:repositorio.unifesp.br:11600/58646Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-07-31T22:36:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
title Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
spellingShingle Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
Saia, Flavia Talarico [UNIFESP]
Pilot scale reactor
Sewage treatment
Denitrification
Sulfide
Microbial community
Pyrosequencing
title_short Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
title_full Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
title_fullStr Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
title_full_unstemmed Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
title_sort Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
author Saia, Flavia Talarico [UNIFESP]
author_facet Saia, Flavia Talarico [UNIFESP]
Souza, Theo Syrto Octavio de
Duarte, Rubens Tadeu Delgado
Pozzi, Eloisa
Fonseca, Debora
Foresti, Eugenio
author_role author
author2 Souza, Theo Syrto Octavio de
Duarte, Rubens Tadeu Delgado
Pozzi, Eloisa
Fonseca, Debora
Foresti, Eugenio
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Saia, Flavia Talarico [UNIFESP]
Souza, Theo Syrto Octavio de
Duarte, Rubens Tadeu Delgado
Pozzi, Eloisa
Fonseca, Debora
Foresti, Eugenio
dc.subject.eng.fl_str_mv Pilot scale reactor
Sewage treatment
Denitrification
Sulfide
Microbial community
Pyrosequencing
topic Pilot scale reactor
Sewage treatment
Denitrification
Sulfide
Microbial community
Pyrosequencing
description A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2020-11-03T14:40:37Z
dc.date.available.fl_str_mv 2020-11-03T14:40:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016.
dc.identifier.uri.fl_str_mv https://repositorio.unifesp.br/handle/11600/58646
https://doi.org/10.1007/s00449-015-1520-6
dc.identifier.issn.none.fl_str_mv 1615-7591
dc.identifier.doi.none.fl_str_mv 10.1007/s00449-015-1520-6
dc.identifier.wos.none.fl_str_mv WOS:000368723100014
identifier_str_mv Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016.
1615-7591
10.1007/s00449-015-1520-6
WOS:000368723100014
url https://repositorio.unifesp.br/handle/11600/58646
https://doi.org/10.1007/s00449-015-1520-6
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Bioprocess And Biosystems Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 341-352
dc.coverage.none.fl_str_mv New York
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv
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