Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/58646 https://doi.org/10.1007/s00449-015-1520-6 |
Resumo: | A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system. |
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Saia, Flavia Talarico [UNIFESP]Souza, Theo Syrto Octavio deDuarte, Rubens Tadeu DelgadoPozzi, EloisaFonseca, DeboraForesti, Eugenio2020-11-03T14:40:37Z2020-11-03T14:40:37Z2016Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016.1615-7591https://repositorio.unifesp.br/handle/11600/58646https://doi.org/10.1007/s00449-015-1520-610.1007/s00449-015-1520-6WOS:000368723100014A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Sao Paulo, Dept Sea Sci, Av Saldanha Da Gama 89, BR-11030400 Santos, SP, BrazilUniv Sao Paulo, Dept Hydraul & Environm Engn, Polytech Sch, Av Prof Almeida Prado 83,Trav 2,Cidade Univ, BR-05508900 Sao Paulo, SP, BrazilUniv Fed Santa Catarina, Dept Microbiol Immunol & Parasitol, Florianopolis, SC, BrazilUniv Sao Paulo, Sch Engn Sao Carlos, Lab Biol Proc, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, BrazilUniv Fed Sao Paulo, Dept Sea Sci, Av Saldanha Da Gama 89, BR-11030400 Santos, SP, BrazilFAPESP: 2007/58659-7FAPESP: 2010/03286-4FAPESP: 2010/01735-6Web of Science341-352engSpringerBioprocess And Biosystems EngineeringPilot scale reactorSewage treatmentDenitrificationSulfideMicrobial communityPyrosequencingMicrobial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNew York392info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/586462023-07-31 19:36:35.963metadata only accessoai:repositorio.unifesp.br:11600/58646Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-07-31T22:36:35Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
title |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
spellingShingle |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment Saia, Flavia Talarico [UNIFESP] Pilot scale reactor Sewage treatment Denitrification Sulfide Microbial community Pyrosequencing |
title_short |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
title_full |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
title_fullStr |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
title_full_unstemmed |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
title_sort |
Microbial community in a pilot-scale bioreactor promoting anaerobic digestion and sulfur-driven denitrification for domestic sewage treatment |
author |
Saia, Flavia Talarico [UNIFESP] |
author_facet |
Saia, Flavia Talarico [UNIFESP] Souza, Theo Syrto Octavio de Duarte, Rubens Tadeu Delgado Pozzi, Eloisa Fonseca, Debora Foresti, Eugenio |
author_role |
author |
author2 |
Souza, Theo Syrto Octavio de Duarte, Rubens Tadeu Delgado Pozzi, Eloisa Fonseca, Debora Foresti, Eugenio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Saia, Flavia Talarico [UNIFESP] Souza, Theo Syrto Octavio de Duarte, Rubens Tadeu Delgado Pozzi, Eloisa Fonseca, Debora Foresti, Eugenio |
dc.subject.eng.fl_str_mv |
Pilot scale reactor Sewage treatment Denitrification Sulfide Microbial community Pyrosequencing |
topic |
Pilot scale reactor Sewage treatment Denitrification Sulfide Microbial community Pyrosequencing |
description |
A pilot-scale reactor treating domestic sewage was operated to promote anaerobic digestion and denitrification using endogenous electron donors. While 55 % of organic matter was removed, nitrogen and sulfur showed a different dynamics during the operation. Pyrosequencing analysis clarified this behavior revealing that specific microbial communities inhabited the anaerobic (47.05 % of OTUs) and anoxic (31.39 % of OTUs) chambers. Analysis of 16S rRNA gene partial sequences obtained through pyrosequencing revealed a total of 1727 OTUs clustered at a 3 % distance cutoff. In the anaerobic chamber, microbial community was comprised of fermentative, syntrophic and sulfate-reducing bacteria. The majority of sequences were related to Aminobacterium and Syntrophorhabdus. In the anoxic chamber, the majority of sequences were related to mixotrophic and strictly autotrophic denitrifiers Arcobacter and Sulfuricurvum, respectively, both involved in sulfur-driven denitrification. These results show that pyrosequencing was a powerful tool to investigate the microbial panorama of a complex system, providing new insights to the improvement of the system. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-11-03T14:40:37Z |
dc.date.available.fl_str_mv |
2020-11-03T14:40:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/58646 https://doi.org/10.1007/s00449-015-1520-6 |
dc.identifier.issn.none.fl_str_mv |
1615-7591 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s00449-015-1520-6 |
dc.identifier.wos.none.fl_str_mv |
WOS:000368723100014 |
identifier_str_mv |
Bioprocess And Biosystems Engineering. New York, v. 39, n. 2, p. 341-352, 2016. 1615-7591 10.1007/s00449-015-1520-6 WOS:000368723100014 |
url |
https://repositorio.unifesp.br/handle/11600/58646 https://doi.org/10.1007/s00449-015-1520-6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Bioprocess And Biosystems Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
341-352 |
dc.coverage.none.fl_str_mv |
New York |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460336560504832 |