Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34375 http://dx.doi.org/10.1155/2012/649353 |
Resumo: | Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios. |
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Janz, Felipe de LaraDebes, Adriana de AguiarCavaglieri, Rita de CassiaDuarte, Sergio AloisioRomao, Carolina MartinezMoron, Antonio Fernandes [UNIFESP]Zugaib, Marcelo [UNIFESP]Bydlowski, Sergio PauloUniversidade de São Paulo (USP)Fundacao Prosangue Hemoctr São PauloUniversidade Federal de São Paulo (UNIFESP)2016-01-24T14:17:37Z2016-01-24T14:17:37Z2012-01-01Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012.1110-7243http://repositorio.unifesp.br/handle/11600/34375http://dx.doi.org/10.1155/2012/649353WOS000304937000001.pdf10.1155/2012/649353WOS:000304937000001Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Financiadora de Estudos e Projetos (FINEP)Instituto Nacional de Ciencia e Tecnologia-Fluidos Complexos (INCT-FCx), BrazilUniv São Paulo, Sch Med, Lab Genet & Mol Hematol LIM 31, BR-05403000 São Paulo, BrazilFundacao Prosangue Hemoctr São Paulo, BR-05403000 São Paulo, BrazilUniv São Paulo, Sch Med, Dept Gynecol & Obstet, BR-05403000 São Paulo, BrazilUniversidade Federal de São Paulo, Sch Med, Dept Obstet, BR-04021001 São Paulo, BrazilUniversidade Federal de São Paulo, Sch Med, Dept Obstet, BR-04021001 São Paulo, BrazilWeb of Science10engHindawi Publishing CorporationJournal of Biomedicine and BiotechnologyEvaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000304937000001.pdfapplication/pdf3066407${dspace.ui.url}/bitstream/11600/34375/1/WOS000304937000001.pdf4ce891682bb694a2fe001d5285f0d436MD51open accessTEXTWOS000304937000001.pdf.txtWOS000304937000001.pdf.txtExtracted texttext/plain40857${dspace.ui.url}/bitstream/11600/34375/2/WOS000304937000001.pdf.txt317f06f35fa74d6be1177b496e1cb05cMD52open access11600/343752023-05-10 13:54:11.11open accessoai:repositorio.unifesp.br:11600/34375Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-10T16:54:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
title |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
spellingShingle |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation Janz, Felipe de Lara |
title_short |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
title_full |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
title_fullStr |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
title_full_unstemmed |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
title_sort |
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation |
author |
Janz, Felipe de Lara |
author_facet |
Janz, Felipe de Lara Debes, Adriana de Aguiar Cavaglieri, Rita de Cassia Duarte, Sergio Aloisio Romao, Carolina Martinez Moron, Antonio Fernandes [UNIFESP] Zugaib, Marcelo [UNIFESP] Bydlowski, Sergio Paulo |
author_role |
author |
author2 |
Debes, Adriana de Aguiar Cavaglieri, Rita de Cassia Duarte, Sergio Aloisio Romao, Carolina Martinez Moron, Antonio Fernandes [UNIFESP] Zugaib, Marcelo [UNIFESP] Bydlowski, Sergio Paulo |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Fundacao Prosangue Hemoctr São Paulo Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Janz, Felipe de Lara Debes, Adriana de Aguiar Cavaglieri, Rita de Cassia Duarte, Sergio Aloisio Romao, Carolina Martinez Moron, Antonio Fernandes [UNIFESP] Zugaib, Marcelo [UNIFESP] Bydlowski, Sergio Paulo |
description |
Amniotic fluid (AF) was described as a potential source of mesenchymal stem cells (MSCs) for biomedicine purposes. Therefore, evaluation of alternative cryoprotectants and freezing protocols capable to maintain the viability and stemness of these cells after cooling is still needed. AF stem cells (AFSCs) were tested for different freezing methods and cryoprotectants. Cell viability, gene expression, surface markers, and plasticity were evaluated after thawing. AFSCs expressed undifferentiated genes Oct4 and Nanog; presented typical markers (CD29, CD44, CD90, and CD105) and were able to differentiate into mesenchymal lineages. All tested cryoprotectants preserved the features of AFSCs however, variations in cell viability were observed. in this concern, dimethyl sulfoxide (Me2SO) showed the best results. the freezing protocols tested did not promote significant changes in the AFSCs viability. Time programmed and nonprogrammed freezing methods could be used for successful AFSCs cryopreservation for 6 months. Although tested cryoprotectants maintained undifferentiated gene expression, typical markers, and plasticity of AFSCs, only Me2SO and glycerol presented workable viability ratios. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:17:37Z |
dc.date.available.fl_str_mv |
2016-01-24T14:17:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34375 http://dx.doi.org/10.1155/2012/649353 |
dc.identifier.issn.none.fl_str_mv |
1110-7243 |
dc.identifier.file.none.fl_str_mv |
WOS000304937000001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1155/2012/649353 |
dc.identifier.wos.none.fl_str_mv |
WOS:000304937000001 |
identifier_str_mv |
Journal of Biomedicine and Biotechnology. New York: Hindawi Publishing Corporation, 10 p., 2012. 1110-7243 WOS000304937000001.pdf 10.1155/2012/649353 WOS:000304937000001 |
url |
http://repositorio.unifesp.br/handle/11600/34375 http://dx.doi.org/10.1155/2012/649353 |
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eng |
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10 |
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Hindawi Publishing Corporation |
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Hindawi Publishing Corporation |
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