Comparative study of the stability of free and modified papain incorporated in topical formulations
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S1984-82502011000400012 http://repositorio.unifesp.br/handle/11600/6766 |
Resumo: | Papain is an enzyme used in topical formulations as a proteolytic debriding agent for the treatment of open, extensive wounds and burnings. It is also employed as an enhancer for cutaneous permeation of active compounds, chemical peeling and as a progressive depilatory agent. The stability of formulations containing enzymes is not easy. In this research, papain was modified with polyethylene glycol in order to increase the stability of the formulations. The comparative Normal Stability Testing of the topical formulations containing unmodified and modified papain showed that the modified variety presented with a differentiated profile under the adopted temperature conditions (5.0 ± 1.0 °C; 22.0 ± 2.0 °C; 40.0 ± 2.0 °C). The most suitable condition for non-modified papain were 5.0 ± 1.0 °C and, for modified papain, they were 22.0 ± 2.0 °C. These results confirmed the higher stability of modified papain compared to free papain, as well as its potential to be applied in topical formulations. |
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Comparative study of the stability of free and modified papain incorporated in topical formulationsPapainPoliethylene glycolTopical formulationsPapainaPolietilenoglicolFormulações tópicasPapain is an enzyme used in topical formulations as a proteolytic debriding agent for the treatment of open, extensive wounds and burnings. It is also employed as an enhancer for cutaneous permeation of active compounds, chemical peeling and as a progressive depilatory agent. The stability of formulations containing enzymes is not easy. In this research, papain was modified with polyethylene glycol in order to increase the stability of the formulations. The comparative Normal Stability Testing of the topical formulations containing unmodified and modified papain showed that the modified variety presented with a differentiated profile under the adopted temperature conditions (5.0 ± 1.0 °C; 22.0 ± 2.0 °C; 40.0 ± 2.0 °C). The most suitable condition for non-modified papain were 5.0 ± 1.0 °C and, for modified papain, they were 22.0 ± 2.0 °C. These results confirmed the higher stability of modified papain compared to free papain, as well as its potential to be applied in topical formulations.A papaína é uma enzima utilizada em formulações tópicas como agente proteolítico debridante no tratamento de lesões abertas de grande extensão e queimaduras. É, também, empregada na pele íntegra como agente promotor da permeação cutânea de princípios ativos, peeling químico e como agente depilatório progressivo. A estabilidade de formulações contendo enzimas não é facilmente alcançada. No presente trabalho realizou-se a modificação da enzima com polietilenoglicol, visando maior estabilidade das formulações. A realização do Teste Estabilidade Normal comparativo entre as formulações contendo as formas da enzima não modificada e modificada demonstrou que a última apresentou um perfil de estabilidade diferenciado, nas diferentes condições (5,0 ± 1,0 °C; 22,0 ± 2,0 °C; 40,0 ± 2,0 °C). A condição de 5,0 ± 1,0 °C foi a mais adequada para a formulação contendo papaína não modificada enquanto a 22,0 ± 2,0 °C foi indicada para aquela contendo a forma modificada. Estes resultados confirmaram o aumento da estabilidade da papaína modificada comparada com a livre e seu potencial de aplicação em formulações de uso tópico.University of São Paulo Faculty of Pharmaceutical Sciences Department of PharmacyUniversidade Federal de São Paulo (UNIFESP) Instituto de Ciências Ambientais, Químicas e Farmacêuticas Departamento de Ciências Exatas e da TerraUniversity of São Paulo Faculty of Pharmaceutical Sciences Department of Pharmaceutical and Biochemical TechnologyUNIFESP, Instituto de Ciências Ambientais, Químicas e Farmacêuticas Depto. de Ciências Exatas e da TerraSciELOUniversidade de São Paulo, Faculdade de Ciências FarmacêuticasUniversity of São Paulo Faculty of Pharmaceutical Sciences Department of PharmacyUniversidade Federal de São Paulo (UNIFESP)University of São Paulo Faculty of Pharmaceutical Sciences Department of Pharmaceutical and Biochemical TechnologyPinto, Claudinéia Aparecida Sales De OliveiraLopes, Patricia Santos [UNIFESP]Sarruf, Fernanda DaudPolakiewicz, BronislawKaneko, Telma MaryBaby, Andre RolimVelasco, Maria Valéria Robles2015-06-14T13:43:25Z2015-06-14T13:43:25Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion751-760application/pdfhttp://dx.doi.org/10.1590/S1984-82502011000400012Brazilian Journal of Pharmaceutical Sciences. Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, v. 47, n. 4, p. 751-760, 2011.10.1590/S1984-82502011000400012S1984-82502011000400012.pdf1984-8250S1984-82502011000400012http://repositorio.unifesp.br/handle/11600/6766WOS:000302521000012engBrazilian Journal of Pharmaceutical Sciencesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T23:36:23Zoai:repositorio.unifesp.br/:11600/6766Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T23:36:23Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| title |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| spellingShingle |
Comparative study of the stability of free and modified papain incorporated in topical formulations Pinto, Claudinéia Aparecida Sales De Oliveira Papain Poliethylene glycol Topical formulations Papaina Polietilenoglicol Formulações tópicas |
| title_short |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| title_full |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| title_fullStr |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| title_full_unstemmed |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| title_sort |
Comparative study of the stability of free and modified papain incorporated in topical formulations |
| author |
Pinto, Claudinéia Aparecida Sales De Oliveira |
| author_facet |
Pinto, Claudinéia Aparecida Sales De Oliveira Lopes, Patricia Santos [UNIFESP] Sarruf, Fernanda Daud Polakiewicz, Bronislaw Kaneko, Telma Mary Baby, Andre Rolim Velasco, Maria Valéria Robles |
| author_role |
author |
| author2 |
Lopes, Patricia Santos [UNIFESP] Sarruf, Fernanda Daud Polakiewicz, Bronislaw Kaneko, Telma Mary Baby, Andre Rolim Velasco, Maria Valéria Robles |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
University of São Paulo Faculty of Pharmaceutical Sciences Department of Pharmacy Universidade Federal de São Paulo (UNIFESP) University of São Paulo Faculty of Pharmaceutical Sciences Department of Pharmaceutical and Biochemical Technology |
| dc.contributor.author.fl_str_mv |
Pinto, Claudinéia Aparecida Sales De Oliveira Lopes, Patricia Santos [UNIFESP] Sarruf, Fernanda Daud Polakiewicz, Bronislaw Kaneko, Telma Mary Baby, Andre Rolim Velasco, Maria Valéria Robles |
| dc.subject.por.fl_str_mv |
Papain Poliethylene glycol Topical formulations Papaina Polietilenoglicol Formulações tópicas |
| topic |
Papain Poliethylene glycol Topical formulations Papaina Polietilenoglicol Formulações tópicas |
| description |
Papain is an enzyme used in topical formulations as a proteolytic debriding agent for the treatment of open, extensive wounds and burnings. It is also employed as an enhancer for cutaneous permeation of active compounds, chemical peeling and as a progressive depilatory agent. The stability of formulations containing enzymes is not easy. In this research, papain was modified with polyethylene glycol in order to increase the stability of the formulations. The comparative Normal Stability Testing of the topical formulations containing unmodified and modified papain showed that the modified variety presented with a differentiated profile under the adopted temperature conditions (5.0 ± 1.0 °C; 22.0 ± 2.0 °C; 40.0 ± 2.0 °C). The most suitable condition for non-modified papain were 5.0 ± 1.0 °C and, for modified papain, they were 22.0 ± 2.0 °C. These results confirmed the higher stability of modified papain compared to free papain, as well as its potential to be applied in topical formulations. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-12-01 2015-06-14T13:43:25Z 2015-06-14T13:43:25Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-82502011000400012 Brazilian Journal of Pharmaceutical Sciences. Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, v. 47, n. 4, p. 751-760, 2011. 10.1590/S1984-82502011000400012 S1984-82502011000400012.pdf 1984-8250 S1984-82502011000400012 http://repositorio.unifesp.br/handle/11600/6766 WOS:000302521000012 |
| url |
http://dx.doi.org/10.1590/S1984-82502011000400012 http://repositorio.unifesp.br/handle/11600/6766 |
| identifier_str_mv |
Brazilian Journal of Pharmaceutical Sciences. Universidade de São Paulo, Faculdade de Ciências Farmacêuticas, v. 47, n. 4, p. 751-760, 2011. 10.1590/S1984-82502011000400012 S1984-82502011000400012.pdf 1984-8250 S1984-82502011000400012 WOS:000302521000012 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
751-760 application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas |
| publisher.none.fl_str_mv |
Universidade de São Paulo, Faculdade de Ciências Farmacêuticas |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268271216558080 |