Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?

Detalhes bibliográficos
Autor(a) principal: Ferreira, Cecília da Silva [UNIFESP]
Data de Publicação: 2016
Outros Autores: Carvalho, Kátia Cândido [UNIFESP], Maganhin, Carla Cristina [UNIFESP], Paiotti, Ana Paula Ribeiro [UNIFESP], Oshima, Celina Tizuko Fujiyama [UNIFESP], Simões, Manuel de Jesus [UNIFESP], Baracat, Edmund Chada [UNIFESP], Soares Júnior, José Maria [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://doi.org/10.1007/s10495-015-1195-0
https://repositorio.unifesp.br/handle/11600/58519
Resumo: Melatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.
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spelling Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?MelatoninFemale ratsContinuous lightApoptosisMelatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.Univ Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 09/51754-0CNPq: 137442/2009-2Springer2020-10-30T18:46:35Z2020-10-30T18:46:35Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion155-162https://doi.org/10.1007/s10495-015-1195-0Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.10.1007/s10495-015-1195-01360-8185https://repositorio.unifesp.br/handle/11600/58519WOS:000368176900004engApoptosisDordrechtinfo:eu-repo/semantics/openAccessFerreira, Cecília da Silva [UNIFESP]Carvalho, Kátia Cândido [UNIFESP]Maganhin, Carla Cristina [UNIFESP]Paiotti, Ana Paula Ribeiro [UNIFESP]Oshima, Celina Tizuko Fujiyama [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Baracat, Edmund Chada [UNIFESP]Soares Júnior, José Maria [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-10-05T15:45:57Zoai:repositorio.unifesp.br/:11600/58519Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-10-05T15:45:57Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
title Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
spellingShingle Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
Ferreira, Cecília da Silva [UNIFESP]
Melatonin
Female rats
Continuous light
Apoptosis
title_short Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
title_full Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
title_fullStr Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
title_full_unstemmed Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
title_sort Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
author Ferreira, Cecília da Silva [UNIFESP]
author_facet Ferreira, Cecília da Silva [UNIFESP]
Carvalho, Kátia Cândido [UNIFESP]
Maganhin, Carla Cristina [UNIFESP]
Paiotti, Ana Paula Ribeiro [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Soares Júnior, José Maria [UNIFESP]
author_role author
author2 Carvalho, Kátia Cândido [UNIFESP]
Maganhin, Carla Cristina [UNIFESP]
Paiotti, Ana Paula Ribeiro [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Soares Júnior, José Maria [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Cecília da Silva [UNIFESP]
Carvalho, Kátia Cândido [UNIFESP]
Maganhin, Carla Cristina [UNIFESP]
Paiotti, Ana Paula Ribeiro [UNIFESP]
Oshima, Celina Tizuko Fujiyama [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
Soares Júnior, José Maria [UNIFESP]
dc.subject.por.fl_str_mv Melatonin
Female rats
Continuous light
Apoptosis
topic Melatonin
Female rats
Continuous light
Apoptosis
description Melatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-10-30T18:46:35Z
2020-10-30T18:46:35Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1007/s10495-015-1195-0
Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.
10.1007/s10495-015-1195-0
1360-8185
https://repositorio.unifesp.br/handle/11600/58519
WOS:000368176900004
url https://doi.org/10.1007/s10495-015-1195-0
https://repositorio.unifesp.br/handle/11600/58519
identifier_str_mv Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.
10.1007/s10495-015-1195-0
1360-8185
WOS:000368176900004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Apoptosis
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 155-162
dc.coverage.none.fl_str_mv Dordrecht
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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