Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://doi.org/10.1007/s10495-015-1195-0 https://repositorio.unifesp.br/handle/11600/58519 |
Resumo: | Melatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light. |
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Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light?MelatoninFemale ratsContinuous lightApoptosisMelatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light.Univ Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Ginecol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Fac Med, Dept Obstet & Ginecol, Lab Struct & Mol Gynecol LIM 58,Discipline Gineco, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Patol, Lab Pathol Mol, BR-01246923 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Morfol & Genet, BR-01246923 Sao Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 09/51754-0CNPq: 137442/2009-2Springer2020-10-30T18:46:35Z2020-10-30T18:46:35Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion155-162https://doi.org/10.1007/s10495-015-1195-0Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016.10.1007/s10495-015-1195-01360-8185https://repositorio.unifesp.br/handle/11600/58519WOS:000368176900004engApoptosisDordrechtinfo:eu-repo/semantics/openAccessFerreira, Cecília da Silva [UNIFESP]Carvalho, Kátia Cândido [UNIFESP]Maganhin, Carla Cristina [UNIFESP]Paiotti, Ana Paula Ribeiro [UNIFESP]Oshima, Celina Tizuko Fujiyama [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Baracat, Edmund Chada [UNIFESP]Soares Júnior, José Maria [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-10-05T15:45:57Zoai:repositorio.unifesp.br/:11600/58519Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-10-05T15:45:57Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
title |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
spellingShingle |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? Ferreira, Cecília da Silva [UNIFESP] Melatonin Female rats Continuous light Apoptosis |
title_short |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
title_full |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
title_fullStr |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
title_full_unstemmed |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
title_sort |
Does melatonin influence the apoptosis in rat uterus of animals exposed to continuous light? |
author |
Ferreira, Cecília da Silva [UNIFESP] |
author_facet |
Ferreira, Cecília da Silva [UNIFESP] Carvalho, Kátia Cândido [UNIFESP] Maganhin, Carla Cristina [UNIFESP] Paiotti, Ana Paula Ribeiro [UNIFESP] Oshima, Celina Tizuko Fujiyama [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Baracat, Edmund Chada [UNIFESP] Soares Júnior, José Maria [UNIFESP] |
author_role |
author |
author2 |
Carvalho, Kátia Cândido [UNIFESP] Maganhin, Carla Cristina [UNIFESP] Paiotti, Ana Paula Ribeiro [UNIFESP] Oshima, Celina Tizuko Fujiyama [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Baracat, Edmund Chada [UNIFESP] Soares Júnior, José Maria [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Cecília da Silva [UNIFESP] Carvalho, Kátia Cândido [UNIFESP] Maganhin, Carla Cristina [UNIFESP] Paiotti, Ana Paula Ribeiro [UNIFESP] Oshima, Celina Tizuko Fujiyama [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Baracat, Edmund Chada [UNIFESP] Soares Júnior, José Maria [UNIFESP] |
dc.subject.por.fl_str_mv |
Melatonin Female rats Continuous light Apoptosis |
topic |
Melatonin Female rats Continuous light Apoptosis |
description |
Melatonin has been described as a protective agent against cell death and oxidative stress in different tissues, including in the reproductive system. However, the information on the action of this hormone in rat uterine apoptosis is low. Our objective was to evaluate the effects of melatonin on mechanisms of cell death in uterus of rats exposed to continuous light stress. Twenty adult Wistar rats were divided into two groups: GContr (vehicle control) and GExp which were treated with melatonin (0.4 mg/mL), both were exposed to continuous light for 90 days. The uterus was removed and processed for quantitative real time PCR (qRT-PCR), using PCR-array plates of the apoptosis pathway; for immunohistochemistry and TUNEL. The results of qRT-PCR of GEXP group showed up-regulation of 13 and 7, pro-apoptotic and anti-apoptotic genes, respectively, compared to GContr group. No difference in pro-apoptotic proteins (Bax, Fas and Faslg) expression was observed by immunohistochemistry, although the number of TUNEL-positive cells was lower in the group treated with melatonin compared to the group not treated with this hormone. Our data suggest that melatonin influences the mechanism and decreases the apoptosis in uterus of rats exposed to continuous light. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2020-10-30T18:46:35Z 2020-10-30T18:46:35Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1007/s10495-015-1195-0 Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016. 10.1007/s10495-015-1195-0 1360-8185 https://repositorio.unifesp.br/handle/11600/58519 WOS:000368176900004 |
url |
https://doi.org/10.1007/s10495-015-1195-0 https://repositorio.unifesp.br/handle/11600/58519 |
identifier_str_mv |
Apoptosis. Dordrecht, v. 21, n. 2, p. 155-162, 2016. 10.1007/s10495-015-1195-0 1360-8185 WOS:000368176900004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Apoptosis |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
155-162 |
dc.coverage.none.fl_str_mv |
Dordrecht |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268402870517760 |