Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/34810 http://dx.doi.org/10.1016/j.bbamem.2012.01.012 |
Resumo: | Investigating the role of proteoglycans associated to cell membranes is fundamental to comprehend biochemical process that occurs at the level of membrane surfaces. in this paper, we exploit syndecan-4, a heparan sulfate proteoglycan obtained from cell cultures, in lipid Langmuir monolayers at the air-water interface. the monolayer served as a model for half a membrane, and the molecular interactions involved could be evaluated with tensiometry and vibrational spectroscopy techniques. Polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) employed in a constant surface pressure regime showed that the main chemical groups for syndecan-4 were present at the air-water interface. Subsequent monolayer decompression and compression showed surface pressure-area isotherms with a large expansion for the lipid monolayers interacting with the cell culture reported to over-express syndecan-4, which was also an indication that the proteoglycan was inserted in the lipid monolayer. the introduction of biological molecules with affinity for syndecam-4, such as growth factors, which present a key role in biochemical process of cell signaling, changed the surface properties of the hybrid film, leading to a model, by which the growth factor binds to the sulfate groups present in the heparan sulfate chains. the polypeptide moiety of syndecan-4 responds to this interaction changing its conformation, which leads to lipid film relaxation and further monolayer condensation. (C) 2012 Elsevier B.V. All rights reserved. |
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Caseli, Luciano [UNIFESP]Cavalheiro, Renan Pelluzzi [UNIFESP]Nader, Helena Bonciani [UNIFESP]Lopes, Carla Cristina [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:27:08Z2016-01-24T14:27:08Z2012-05-01Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1818, n. 5, p. 1211-1217, 2012.0005-2736http://repositorio.unifesp.br/handle/11600/34810http://dx.doi.org/10.1016/j.bbamem.2012.01.012WOS000302971100011.pdf10.1016/j.bbamem.2012.01.012WOS:000302971100011Investigating the role of proteoglycans associated to cell membranes is fundamental to comprehend biochemical process that occurs at the level of membrane surfaces. in this paper, we exploit syndecan-4, a heparan sulfate proteoglycan obtained from cell cultures, in lipid Langmuir monolayers at the air-water interface. the monolayer served as a model for half a membrane, and the molecular interactions involved could be evaluated with tensiometry and vibrational spectroscopy techniques. Polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) employed in a constant surface pressure regime showed that the main chemical groups for syndecan-4 were present at the air-water interface. Subsequent monolayer decompression and compression showed surface pressure-area isotherms with a large expansion for the lipid monolayers interacting with the cell culture reported to over-express syndecan-4, which was also an indication that the proteoglycan was inserted in the lipid monolayer. the introduction of biological molecules with affinity for syndecam-4, such as growth factors, which present a key role in biochemical process of cell signaling, changed the surface properties of the hybrid film, leading to a model, by which the growth factor binds to the sulfate groups present in the heparan sulfate chains. the polypeptide moiety of syndecan-4 responds to this interaction changing its conformation, which leads to lipid film relaxation and further monolayer condensation. (C) 2012 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, SP, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Diadema, SP, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, SP, BrazilWeb of Science1211-1217engElsevier B.V.Biochimica Et Biophysica Acta-biomembraneshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessHeparan sulfate proteoglycanSyndecan-4Molecular recognizingLangmuir monolayerProbing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000302971100011.pdfapplication/pdf1070186${dspace.ui.url}/bitstream/11600/34810/1/WOS000302971100011.pdfac0964e535834f233c962744017c9a13MD51open accessTEXTWOS000302971100011.pdf.txtWOS000302971100011.pdf.txtExtracted texttext/plain42392${dspace.ui.url}/bitstream/11600/34810/9/WOS000302971100011.pdf.txt5aee41aa8e2cfde798a49ef6bb32ce96MD59open accessTHUMBNAILWOS000302971100011.pdf.jpgWOS000302971100011.pdf.jpgIM Thumbnailimage/jpeg7819${dspace.ui.url}/bitstream/11600/34810/11/WOS000302971100011.pdf.jpga13f18e608e97a4e1c46b0f3ff626f95MD511open access11600/348102023-06-05 19:26:24.182open accessoai:repositorio.unifesp.br:11600/34810Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:26:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
title |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
spellingShingle |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study Caseli, Luciano [UNIFESP] Heparan sulfate proteoglycan Syndecan-4 Molecular recognizing Langmuir monolayer |
title_short |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
title_full |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
title_fullStr |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
title_full_unstemmed |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
title_sort |
Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study |
author |
Caseli, Luciano [UNIFESP] |
author_facet |
Caseli, Luciano [UNIFESP] Cavalheiro, Renan Pelluzzi [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lopes, Carla Cristina [UNIFESP] |
author_role |
author |
author2 |
Cavalheiro, Renan Pelluzzi [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lopes, Carla Cristina [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Caseli, Luciano [UNIFESP] Cavalheiro, Renan Pelluzzi [UNIFESP] Nader, Helena Bonciani [UNIFESP] Lopes, Carla Cristina [UNIFESP] |
dc.subject.eng.fl_str_mv |
Heparan sulfate proteoglycan Syndecan-4 Molecular recognizing Langmuir monolayer |
topic |
Heparan sulfate proteoglycan Syndecan-4 Molecular recognizing Langmuir monolayer |
description |
Investigating the role of proteoglycans associated to cell membranes is fundamental to comprehend biochemical process that occurs at the level of membrane surfaces. in this paper, we exploit syndecan-4, a heparan sulfate proteoglycan obtained from cell cultures, in lipid Langmuir monolayers at the air-water interface. the monolayer served as a model for half a membrane, and the molecular interactions involved could be evaluated with tensiometry and vibrational spectroscopy techniques. Polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) employed in a constant surface pressure regime showed that the main chemical groups for syndecan-4 were present at the air-water interface. Subsequent monolayer decompression and compression showed surface pressure-area isotherms with a large expansion for the lipid monolayers interacting with the cell culture reported to over-express syndecan-4, which was also an indication that the proteoglycan was inserted in the lipid monolayer. the introduction of biological molecules with affinity for syndecam-4, such as growth factors, which present a key role in biochemical process of cell signaling, changed the surface properties of the hybrid film, leading to a model, by which the growth factor binds to the sulfate groups present in the heparan sulfate chains. the polypeptide moiety of syndecan-4 responds to this interaction changing its conformation, which leads to lipid film relaxation and further monolayer condensation. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:27:08Z |
dc.date.available.fl_str_mv |
2016-01-24T14:27:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1818, n. 5, p. 1211-1217, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/34810 http://dx.doi.org/10.1016/j.bbamem.2012.01.012 |
dc.identifier.issn.none.fl_str_mv |
0005-2736 |
dc.identifier.file.none.fl_str_mv |
WOS000302971100011.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bbamem.2012.01.012 |
dc.identifier.wos.none.fl_str_mv |
WOS:000302971100011 |
identifier_str_mv |
Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1818, n. 5, p. 1211-1217, 2012. 0005-2736 WOS000302971100011.pdf 10.1016/j.bbamem.2012.01.012 WOS:000302971100011 |
url |
http://repositorio.unifesp.br/handle/11600/34810 http://dx.doi.org/10.1016/j.bbamem.2012.01.012 |
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eng |
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eng |
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Biochimica Et Biophysica Acta-biomembranes |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
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1211-1217 |
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Elsevier B.V. |
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Elsevier B.V. |
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