Potencial terapêutico cardíaco das células tronco mesenquimais precursoras de medula óssea CD271+

• Autor(a) principal: Zuttion, Marilia Sanches Santos Rizzo [UNIFESP]
• Data de Publicação: 2018
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=7023888; https://repositorio.unifesp.br/handle/11600/52334
• topic: Trunk cell of bone marrow; CD271 + Trunk Cell; Myocardial infarction; Célula tronco de medula óssea; Célula tronco CD271+; Infarto do miocárdio

MSCs are a particularly promising bone marrow-derived cell for cardiac regenerative therapy because of their availability, immunologic properties, and record of safety and efficacy. Several studies have confirmed the specificity of CD271 on MSC isolated from bone marrow. This marker has been suggested as a versatile marker to selectively isolate multipotent MSC with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. Moreover MSC are preferentially selected in the CD271+ fraction compared to the CD271− fraction. Objective: We hypothesized that CD271+ MSCs isolated from normal human BM will repair ischemic heart damage in a mouse myocardial infarction (MI) model. Methods: CD271+ cells were isolated and injected in NOD/SCID mice following induction of a myocardial infarction (MI) and the effects on cardiac function and hemodynamic were determined by echocardiography and PV loop, respectively. MI size was calculated by dividing the length of MI, defined as the region with >50% of myocardial wall thickness with collagen (blue) deposition with left ventricle length through MIQuant software and the human injected CD271+ cells were tracked by immunofluorescence using anti-human mitochondrial ribosomal protein L11. The data were performed using the SIGMAPLOT software and the values were expressed as mean±standard error of the mean (SEM). The level of statistical significance was set at p<0.05.Results: The CD271+ cells were capable to adhere at the plastic and showed a high capacity of expansion in vitro. Culture of the CD271+ cells under adherent conditions were not able to differentiate in osteo and adipogenic lineages. Animals treated with CD271+ cells had no significant improvement in heart function, however it demonstrated a tendency to preserve EF. Moreover, it was possible to track human cells at the infarcted zone in cell treated animals. Conclusion: We conclude that CD271 inhibited the differentiation of this cells into osteo and adipogenic lineages. These results indicate a role for CD271 in inhibiting the differentiation of MSC CD271+ and the intracardiac injected CD271+ MSC were able to home to infarcted zone after 8 weeks post-MI and demonstrate a trend to preserve EF.