Mitochondrial alterations and apoptosis in smooth muscle from aged rats
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/27978 http://dx.doi.org/10.1016/j.bbabio.2004.05.011 |
Resumo: | We studied changes in mitochondrial morphology and function in the smooth muscle of rat colon. Under confocal microscopy, tissues loaded with potentiometric dye displayed rapid and spontaneous depolarization. Cyclosporin A (CsA), inhibitor of the permeability transition pore (PTP), caused an increase in mitochondrial membrane potential (DeltaPsi(m)) in tissues from adult young animals. in aged rats these changes were not observed. This suggests that physiological activation of PTP in aged rats is reduced. Electron microscopy showed alterations of the mitochondrial ultrastructure in tissues from aged rats involving a decreased definition of the cristae and fragmentation of the mitochondrial membranes. We also detected an increase in apoptotic cells in the smooth muscle from aged animals. Our results show that the aging process changes PTP activity, the ability to maintain DeltaPsi(m) and mitochondrial morphology. It is suggested that these can be associated with mitochondrial damage and cell death. (C) 2004 Elsevier B.V. All rights reserved. |
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Lopes, Guiomar Silva [UNIFESP]Mora, Oswaldo Alves [UNIFESP]Cerri, Paulo Sergio [UNIFESP]Faria, Flavio Paulo de [UNIFESP]Jurkiewicz, Neide Hyppolito [UNIFESP]Jurkiewicz, AronSmaili, Soraya Soubhi [UNIFESP]Universidade Federal de São Paulo (UNIFESP)UNESP2016-01-24T12:37:26Z2016-01-24T12:37:26Z2004-10-04Biochimica Et Biophysica Acta-bioenergetics. Amsterdam: Elsevier B.V., v. 1658, n. 3, p. 187-194, 2004.0005-2728http://repositorio.unifesp.br/handle/11600/27978http://dx.doi.org/10.1016/j.bbabio.2004.05.011WOS000224338200002.pdf10.1016/j.bbabio.2004.05.011WOS:000224338200002We studied changes in mitochondrial morphology and function in the smooth muscle of rat colon. Under confocal microscopy, tissues loaded with potentiometric dye displayed rapid and spontaneous depolarization. Cyclosporin A (CsA), inhibitor of the permeability transition pore (PTP), caused an increase in mitochondrial membrane potential (DeltaPsi(m)) in tissues from adult young animals. in aged rats these changes were not observed. This suggests that physiological activation of PTP in aged rats is reduced. Electron microscopy showed alterations of the mitochondrial ultrastructure in tissues from aged rats involving a decreased definition of the cristae and fragmentation of the mitochondrial membranes. We also detected an increase in apoptotic cells in the smooth muscle from aged animals. Our results show that the aging process changes PTP activity, the ability to maintain DeltaPsi(m) and mitochondrial morphology. It is suggested that these can be associated with mitochondrial damage and cell death. (C) 2004 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo, Nat Inst Pharmacol, Dept Pharmacol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilUNESP, Sch Dent, Dept Morphol, Araraquara, BrazilUniversidade Federal de São Paulo, Nat Inst Pharmacol, Dept Pharmacol, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilWeb of Science187-194engElsevier B.V.Biochimica Et Biophysica Acta-bioenergeticshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessmitochondriacalciumsmooth muscleagingmitochondrial dysfunctionMitochondrial alterations and apoptosis in smooth muscle from aged ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000224338200002.pdfapplication/pdf415560${dspace.ui.url}/bitstream/11600/27978/1/WOS000224338200002.pdfe92dbd4947e5d80cc891c0a6a032115fMD51open accessTEXTWOS000224338200002.pdf.txtWOS000224338200002.pdf.txtExtracted texttext/plain34735${dspace.ui.url}/bitstream/11600/27978/2/WOS000224338200002.pdf.txt1e1c54ff7f716cf61d00242f44a50d40MD52open access11600/279782022-09-27 10:14:41.904open accessoai:repositorio.unifesp.br:11600/27978Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T13:14:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
title |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
spellingShingle |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats Lopes, Guiomar Silva [UNIFESP] mitochondria calcium smooth muscle aging mitochondrial dysfunction |
title_short |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
title_full |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
title_fullStr |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
title_full_unstemmed |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
title_sort |
Mitochondrial alterations and apoptosis in smooth muscle from aged rats |
author |
Lopes, Guiomar Silva [UNIFESP] |
author_facet |
Lopes, Guiomar Silva [UNIFESP] Mora, Oswaldo Alves [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Faria, Flavio Paulo de [UNIFESP] Jurkiewicz, Neide Hyppolito [UNIFESP] Jurkiewicz, Aron Smaili, Soraya Soubhi [UNIFESP] |
author_role |
author |
author2 |
Mora, Oswaldo Alves [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Faria, Flavio Paulo de [UNIFESP] Jurkiewicz, Neide Hyppolito [UNIFESP] Jurkiewicz, Aron Smaili, Soraya Soubhi [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) UNESP |
dc.contributor.author.fl_str_mv |
Lopes, Guiomar Silva [UNIFESP] Mora, Oswaldo Alves [UNIFESP] Cerri, Paulo Sergio [UNIFESP] Faria, Flavio Paulo de [UNIFESP] Jurkiewicz, Neide Hyppolito [UNIFESP] Jurkiewicz, Aron Smaili, Soraya Soubhi [UNIFESP] |
dc.subject.eng.fl_str_mv |
mitochondria calcium smooth muscle aging mitochondrial dysfunction |
topic |
mitochondria calcium smooth muscle aging mitochondrial dysfunction |
description |
We studied changes in mitochondrial morphology and function in the smooth muscle of rat colon. Under confocal microscopy, tissues loaded with potentiometric dye displayed rapid and spontaneous depolarization. Cyclosporin A (CsA), inhibitor of the permeability transition pore (PTP), caused an increase in mitochondrial membrane potential (DeltaPsi(m)) in tissues from adult young animals. in aged rats these changes were not observed. This suggests that physiological activation of PTP in aged rats is reduced. Electron microscopy showed alterations of the mitochondrial ultrastructure in tissues from aged rats involving a decreased definition of the cristae and fragmentation of the mitochondrial membranes. We also detected an increase in apoptotic cells in the smooth muscle from aged animals. Our results show that the aging process changes PTP activity, the ability to maintain DeltaPsi(m) and mitochondrial morphology. It is suggested that these can be associated with mitochondrial damage and cell death. (C) 2004 Elsevier B.V. All rights reserved. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004-10-04 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:37:26Z |
dc.date.available.fl_str_mv |
2016-01-24T12:37:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Biochimica Et Biophysica Acta-bioenergetics. Amsterdam: Elsevier B.V., v. 1658, n. 3, p. 187-194, 2004. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/27978 http://dx.doi.org/10.1016/j.bbabio.2004.05.011 |
dc.identifier.issn.none.fl_str_mv |
0005-2728 |
dc.identifier.file.none.fl_str_mv |
WOS000224338200002.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.bbabio.2004.05.011 |
dc.identifier.wos.none.fl_str_mv |
WOS:000224338200002 |
identifier_str_mv |
Biochimica Et Biophysica Acta-bioenergetics. Amsterdam: Elsevier B.V., v. 1658, n. 3, p. 187-194, 2004. 0005-2728 WOS000224338200002.pdf 10.1016/j.bbabio.2004.05.011 WOS:000224338200002 |
url |
http://repositorio.unifesp.br/handle/11600/27978 http://dx.doi.org/10.1016/j.bbabio.2004.05.011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Biochimica Et Biophysica Acta-bioenergetics |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
187-194 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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