Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/s0102-865020170110000008 https://repositorio.unifesp.br/handle/11600/58222 |
Resumo: | Purpose: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. Methods: Adult Wistar male rats were randomly (n= 8), anesthetized and divided in: Sham: submitted to operation only; group SS+ IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+ IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+ I+ AT+ R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA. Results: The group SS+ IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p= 0.001) in the heart tissue. The tumor necrosis factoralpha level in plasma decrease in the treated groups when compared with SS+ IR group (p= 0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats. Conclusion: Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion. |
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Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusionAdrenergic AntagonistsAtenololIschemiaReperfusionCytokinesOxidative StressRatsPurpose: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. Methods: Adult Wistar male rats were randomly (n= 8), anesthetized and divided in: Sham: submitted to operation only; group SS+ IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+ IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+ I+ AT+ R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA. Results: The group SS+ IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p= 0.001) in the heart tissue. The tumor necrosis factoralpha level in plasma decrease in the treated groups when compared with SS+ IR group (p= 0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats. Conclusion: Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion.Univ Fed Sao Paulo, UNIFESP, Postgrad Program Translat Med, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg, Div Anesthesia Pain & Intens Med, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Postgrad Program Pharmacol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Pharmacol, Sao Paulo, SP, BrazilUNESP, Vet Med Sch, Aracatuba, SP, BrazilUniv Fed Sao Paulo, Dept Surg, Div Cardiol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Postgrad Program Translat Med, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, UNIFESP, Postgrad Program Translat Med, Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Surg, Div Anesthesia Pain & Intens Med, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Postgrad Program Pharmacol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Pharmacol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Surg, Div Cardiol, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Postgrad Program Translat Med, Sao Paulo, SP, BrazilWeb of ScienceFAPESPActa Cirurgica Brasileira2020-09-01T13:21:21Z2020-09-01T13:21:21Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion964-972application/pdfhttp://dx.doi.org/10.1590/s0102-865020170110000008Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 964-972, 2017.10.1590/s0102-865020170110000008S0102-86502017001100964.pdf0102-8650S0102-86502017001100964https://repositorio.unifesp.br/handle/11600/58222WOS:000417846700008engActa Cirurgica BrasileiraSao Pauloinfo:eu-repo/semantics/openAccessOkada, Mieko [UNIFESP]Reis Falcao, Luiz Fernando [UNIFESP]Ferez, David [UNIFESP]Martins, Jose Luiz [UNIFESP]Errante, Paolo Ruggero [UNIFESP]Menezes Rodrigues, Francisco Sandro [UNIFESP]Caricati-Neto, Afonso [UNIFESP]Marinho, MarciaFenelon, Guilherme [UNIFESP]Oliveira-Junior, Itamar Souza [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T22:55:48Zoai:repositorio.unifesp.br/:11600/58222Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T22:55:48Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
title |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
spellingShingle |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion Okada, Mieko [UNIFESP] Adrenergic Antagonists Atenolol Ischemia Reperfusion Cytokines Oxidative Stress Rats |
title_short |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
title_full |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
title_fullStr |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
title_full_unstemmed |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
title_sort |
Effect of atenolol pre-treatment in heart damage in a model of intestinal ischemia-reperfusion |
author |
Okada, Mieko [UNIFESP] |
author_facet |
Okada, Mieko [UNIFESP] Reis Falcao, Luiz Fernando [UNIFESP] Ferez, David [UNIFESP] Martins, Jose Luiz [UNIFESP] Errante, Paolo Ruggero [UNIFESP] Menezes Rodrigues, Francisco Sandro [UNIFESP] Caricati-Neto, Afonso [UNIFESP] Marinho, Marcia Fenelon, Guilherme [UNIFESP] Oliveira-Junior, Itamar Souza [UNIFESP] |
author_role |
author |
author2 |
Reis Falcao, Luiz Fernando [UNIFESP] Ferez, David [UNIFESP] Martins, Jose Luiz [UNIFESP] Errante, Paolo Ruggero [UNIFESP] Menezes Rodrigues, Francisco Sandro [UNIFESP] Caricati-Neto, Afonso [UNIFESP] Marinho, Marcia Fenelon, Guilherme [UNIFESP] Oliveira-Junior, Itamar Souza [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Okada, Mieko [UNIFESP] Reis Falcao, Luiz Fernando [UNIFESP] Ferez, David [UNIFESP] Martins, Jose Luiz [UNIFESP] Errante, Paolo Ruggero [UNIFESP] Menezes Rodrigues, Francisco Sandro [UNIFESP] Caricati-Neto, Afonso [UNIFESP] Marinho, Marcia Fenelon, Guilherme [UNIFESP] Oliveira-Junior, Itamar Souza [UNIFESP] |
dc.subject.por.fl_str_mv |
Adrenergic Antagonists Atenolol Ischemia Reperfusion Cytokines Oxidative Stress Rats |
topic |
Adrenergic Antagonists Atenolol Ischemia Reperfusion Cytokines Oxidative Stress Rats |
description |
Purpose: To investigate the effects of atenolol in inflammatory mediator and oxidative stress in a myocardial injury by intestinal ischemia/reperfusion in rat model. Methods: Adult Wistar male rats were randomly (n= 8), anesthetized and divided in: Sham: submitted to operation only; group SS+ IR: intravenous saline infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); group AT+ IR: intravenous atenolol infusion (2 mg/kg) following superior mesenteric artery occlusion during 60 minutes (ischemia) and open for 120 minutes (reperfusion); and group AT+ I+ AT+ R: intravenous atenolol infusion following superior mesenteric artery occlusion during 60 minutes (ischemia) and in the time 45 minutes other atenolol doses were administrated and the artery was open for 120 minutes (reperfusion), all animals were submitted to muscular relaxation for mechanical ventilation. In the end of experiment the animals were euthanized and the hearts tissue were morphology analyzed by histology and malondialdehyde by ELISA, and the plasma were analyzed for tumor necrosis factor-alpha by ELISA. Results: The group SS+ IR demonstrated the higher malondialdehyde levels when compared with the atenolol treated-groups (p= 0.001) in the heart tissue. The tumor necrosis factoralpha level in plasma decrease in the treated groups when compared with SS+ IR group (p= 0.001). Histology analyses demonstrate pyknosis, edema, cellular vacuolization, presence of inflammatory infiltrate and band contraction in the heart tissue of the rats. Conclusion: Atenolol significantly reduce the degree of cardiac damage after intestinal ischemia-reperfusion. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2020-09-01T13:21:21Z 2020-09-01T13:21:21Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s0102-865020170110000008 Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 964-972, 2017. 10.1590/s0102-865020170110000008 S0102-86502017001100964.pdf 0102-8650 S0102-86502017001100964 https://repositorio.unifesp.br/handle/11600/58222 WOS:000417846700008 |
url |
http://dx.doi.org/10.1590/s0102-865020170110000008 https://repositorio.unifesp.br/handle/11600/58222 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sao Paulo, v. 32, n. 11, p. 964-972, 2017. 10.1590/s0102-865020170110000008 S0102-86502017001100964.pdf 0102-8650 S0102-86502017001100964 WOS:000417846700008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
964-972 application/pdf |
dc.coverage.none.fl_str_mv |
Sao Paulo |
dc.publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268359604174848 |