Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia

Detalhes bibliográficos
Autor(a) principal: Barros, Jeison de Nadai [UNIFESP]
Data de Publicação: 2014
Outros Autores: Lowen, Marcia Serva [UNIFESP], Moraes-Filho, Milton Nunes de [UNIFESP], Martins, Maria Cristina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.5935/0004-2749.20140077
http://repositorio.unifesp.br/handle/11600/8606
Resumo: Purpose: To evaluate the agreement between the methodologies of impression cytology (IC) and histopathology regarding epithelial lesions clinically diagnosed as pterygium and also regarding the detection of unsuspected and associated ocular surface squamous neoplasia (OSSN). Methods: Thirty-two Brazilian patients were included and IC was performed on all pterygia before excision. Histopathogical examination was considered the gold standard and was performed by two experienced ocular pathologists in which consensus existed regarding pterygia diagnosis. IC accuracy was assessed by sensitivity and specificity with a 95% confidence interval. Results: From the 32 primary lesions studied, histopathological examination confirmed the diagnosis of pterygium without atypical cells in 19 cases (60%) and showed unsuspected and associated OSSN cells in 13 cases (40%). IC demonstrated one false-negative and one false-positive result for atypia. Statistical analysis showed an estimated sensitivity of 92%, specificity of 94%, positive predictive value of 92%, and negative predictive value of 94%. Conclusion: IC demonstrated high agreement with histopathological analysis in the detection of atypical epithelial cells in unsuspected OSSN in Brazilian pterygia patients.
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spelling Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygiaUso da citologia de impressão para a detecção de células de neoplasia escamosa da superfície ocular insuspeita em pterígiosPterygiumConjunctivaCorneaLimbus corneaeOcular surfaceEye neoplasmsCytological techniquesPterígioConjuntivaCórneaLimbo da córneaSuperfície ocularNeoplasias ocularesTécnicas citológicasPurpose: To evaluate the agreement between the methodologies of impression cytology (IC) and histopathology regarding epithelial lesions clinically diagnosed as pterygium and also regarding the detection of unsuspected and associated ocular surface squamous neoplasia (OSSN). Methods: Thirty-two Brazilian patients were included and IC was performed on all pterygia before excision. Histopathogical examination was considered the gold standard and was performed by two experienced ocular pathologists in which consensus existed regarding pterygia diagnosis. IC accuracy was assessed by sensitivity and specificity with a 95% confidence interval. Results: From the 32 primary lesions studied, histopathological examination confirmed the diagnosis of pterygium without atypical cells in 19 cases (60%) and showed unsuspected and associated OSSN cells in 13 cases (40%). IC demonstrated one false-negative and one false-positive result for atypia. Statistical analysis showed an estimated sensitivity of 92%, specificity of 94%, positive predictive value of 92%, and negative predictive value of 94%. Conclusion: IC demonstrated high agreement with histopathological analysis in the detection of atypical epithelial cells in unsuspected OSSN in Brazilian pterygia patients.Objetivo: Avaliar a concordância entre a citologia de impressão (CI) e os achados histopatológicos de lesões epiteliais clinicamente diagnosticadas como pterígio, no que tange a detecção de células de neoplasia escamosa da superfície ocular (NESO) insuspeita associada. Métodos: Trinta e dois pacientes brasileiros foram incluídos e a CI foi obtida de todos os pterígios antes da excisão. O exame histopatológico foi realizado por dois patologistas oculares experientes em consenso de opinião e considerado o padrão-ouro para o diagnóstico. A acurácia da CI foi avaliada pela sensibilidade e especificidade com intervalo de confiança de 95% Resultados: Das 32 lesões estudadas, o exame histopatológico confirmou o diagnóstico de pterígio sem atipia em 19 casos (60%) e mostrou células de NESO insuspeita associada em 13 espécimes (40%). A CI demonstrou um resultado falso-positivo e um falso-negativo para atipia. A análise estatística mostrou uma sensibilidade estimada de 92%, especificidade de 94%, valor preditivo positivo de 92% e valor preditivo negativo de 94%. Conclusão: A CI apresentou alta concordância com o estudo histopatológico na detecção de células epiteliais atípicas de NESO insuspeita em pterígios do Brasil.Universidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM)UNIFESP, EPMSciELOConselho Brasileiro de OftalmologiaUniversidade Federal de São Paulo (UNIFESP)Barros, Jeison de Nadai [UNIFESP]Lowen, Marcia Serva [UNIFESP]Moraes-Filho, Milton Nunes de [UNIFESP]Martins, Maria Cristina [UNIFESP]2015-06-14T13:47:19Z2015-06-14T13:47:19Z2014-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion305-309application/pdfhttp://dx.doi.org/10.5935/0004-2749.20140077Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 77, n. 5, p. 305-309, 2014.10.5935/0004-2749.20140077S0004-27492014000500305.pdf0004-27491678-2925S0004-27492014000500305http://repositorio.unifesp.br/handle/11600/8606WOS:000346167000009engArquivos Brasileiros de Oftalmologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-04T23:04:00Zoai:repositorio.unifesp.br/:11600/8606Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-04T23:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
Uso da citologia de impressão para a detecção de células de neoplasia escamosa da superfície ocular insuspeita em pterígios
title Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
spellingShingle Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
Barros, Jeison de Nadai [UNIFESP]
Pterygium
Conjunctiva
Cornea
Limbus corneae
Ocular surface
Eye neoplasms
Cytological techniques
Pterígio
Conjuntiva
Córnea
Limbo da córnea
Superfície ocular
Neoplasias oculares
Técnicas citológicas
title_short Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
title_full Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
title_fullStr Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
title_full_unstemmed Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
title_sort Use of impression cytology for the detection of unsuspected ocular surface squamous neoplasia cells in pterygia
author Barros, Jeison de Nadai [UNIFESP]
author_facet Barros, Jeison de Nadai [UNIFESP]
Lowen, Marcia Serva [UNIFESP]
Moraes-Filho, Milton Nunes de [UNIFESP]
Martins, Maria Cristina [UNIFESP]
author_role author
author2 Lowen, Marcia Serva [UNIFESP]
Moraes-Filho, Milton Nunes de [UNIFESP]
Martins, Maria Cristina [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Barros, Jeison de Nadai [UNIFESP]
Lowen, Marcia Serva [UNIFESP]
Moraes-Filho, Milton Nunes de [UNIFESP]
Martins, Maria Cristina [UNIFESP]
dc.subject.por.fl_str_mv Pterygium
Conjunctiva
Cornea
Limbus corneae
Ocular surface
Eye neoplasms
Cytological techniques
Pterígio
Conjuntiva
Córnea
Limbo da córnea
Superfície ocular
Neoplasias oculares
Técnicas citológicas
topic Pterygium
Conjunctiva
Cornea
Limbus corneae
Ocular surface
Eye neoplasms
Cytological techniques
Pterígio
Conjuntiva
Córnea
Limbo da córnea
Superfície ocular
Neoplasias oculares
Técnicas citológicas
description Purpose: To evaluate the agreement between the methodologies of impression cytology (IC) and histopathology regarding epithelial lesions clinically diagnosed as pterygium and also regarding the detection of unsuspected and associated ocular surface squamous neoplasia (OSSN). Methods: Thirty-two Brazilian patients were included and IC was performed on all pterygia before excision. Histopathogical examination was considered the gold standard and was performed by two experienced ocular pathologists in which consensus existed regarding pterygia diagnosis. IC accuracy was assessed by sensitivity and specificity with a 95% confidence interval. Results: From the 32 primary lesions studied, histopathological examination confirmed the diagnosis of pterygium without atypical cells in 19 cases (60%) and showed unsuspected and associated OSSN cells in 13 cases (40%). IC demonstrated one false-negative and one false-positive result for atypia. Statistical analysis showed an estimated sensitivity of 92%, specificity of 94%, positive predictive value of 92%, and negative predictive value of 94%. Conclusion: IC demonstrated high agreement with histopathological analysis in the detection of atypical epithelial cells in unsuspected OSSN in Brazilian pterygia patients.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01
2015-06-14T13:47:19Z
2015-06-14T13:47:19Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.5935/0004-2749.20140077
Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 77, n. 5, p. 305-309, 2014.
10.5935/0004-2749.20140077
S0004-27492014000500305.pdf
0004-2749
1678-2925
S0004-27492014000500305
http://repositorio.unifesp.br/handle/11600/8606
WOS:000346167000009
url http://dx.doi.org/10.5935/0004-2749.20140077
http://repositorio.unifesp.br/handle/11600/8606
identifier_str_mv Arquivos Brasileiros de Oftalmologia. Conselho Brasileiro de Oftalmologia, v. 77, n. 5, p. 305-309, 2014.
10.5935/0004-2749.20140077
S0004-27492014000500305.pdf
0004-2749
1678-2925
S0004-27492014000500305
WOS:000346167000009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Oftalmologia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 305-309
application/pdf
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268365368197120

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