IMPs, VIMs and SPMs: the diversity of metallo-beta-lactamases produced by carbapenem-resistant Pseudomonas aeruginosa in a Brazilian hospital

• Autor(a) principal: Sader, Helio Silva [UNIFESP]
• Data de Publicação: 2005
• Outros Autores: Reis, A. O. [UNIFESP], Silbert, Suzane [UNIFESP], Gales, Ana Cristina [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1111/j.1469-0691.2004.01031.x; http://repositorio.unifesp.br/handle/11600/28057
• topic: carbapenem resistance; imipenem resistance; metallo-beta-lactamases; polymyxin; Pseudomonas aeruginosa

Pseudomonas aeruginosa isolates (n = 183), collected from bacteraemic patients hospitalised in São Paulo Hospital (Brazil) during 2000-2001, were screened for susceptibility to antimicrobial agents. the polymyxins were the most active compounds (100% susceptibility), followed by amikacin and cefepime (59.0%), meropenem (57.4%), and imipenem and gentamicin (55.2%). Imipenem-resistant isolates were ribotyped and screened for production of metallo-beta-lactamases (MBLs) by PCR with primers for bla(IMP), bla(VIM) and bla(SPM). MBL production was detected in 36 isolates (19.7% of the entire collection; 43.9% of the imipenem-resistant isolates) and the MBLs included SPM-1-like (55.6%), VIM-2-like (30.6%) and IMP-1-like (8.3%) enzymes.