Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/49371 http://dx.doi.org/10.1155/2016/5976324 |
Resumo: | Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes. The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon (R) (10 kDa porous size) without organic solvent consumption was developed. GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas. DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time. Under chosen parameters (extraction solvent: chloroform, 130 mu L |
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Steinhorst Alcantara, Greyce KellyCalixto, Leandro Augusto [UNIFESP]Beraldo de Moraes, Luiz AlbertoCosta Queiroz, Regina HelenaMoraes de Oliveira, Anderson Rodrigode Gaitani, Cristiane Masetto2019-01-21T10:29:45Z2019-01-21T10:29:45Z2016Journal Of Analytical Methods In Chemistry. London, 2016.2090-8865http://repositorio.unifesp.br/handle/11600/49371http://dx.doi.org/10.1155/2016/5976324WOS000386837800001.pdf10.1155/2016/5976324WOS:000386837800001Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes. The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon (R) (10 kDa porous size) without organic solvent consumption was developed. GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas. DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time. Under chosen parameters (extraction solvent: chloroform, 130 mu Ldispersing solvent: isopropyl alcohol, 400 mu Lno salt addition and no vortex agitation time), the method was completely validated and all parameters were in agreement with the literature recommendations. LEV was quantified in patient's plasma sample using less than 550 mu L of organic solvent.Sao Paulo Research Foundation (FAPESP) [2012/07210-8]Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Department of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, BrazilDepartment of Exact and Earth Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, 09972-270 Diadema, SP, BrazilDepartament of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, 14040-901 Ribeirão Preto, SP, BrazilDepartment of Clinical Analysis, Toxicology and Food Science, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, BrazilDepartment of Exact and Earth Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, 09972-270 Diadema, SP, BrazilFAPESP: 2012/07210-8Web of ScienceengUniv Sao Paulo, Inst PsiquiatriaJournal Of Analytical Methods In ChemistrySolid-Phase ExtractionHplc-UvAntiepileptic DrugsOrganic-CompoundsHuman UrinePerformanceWaterSamplesZonisamideSerumDetermination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000386837800001.pdfapplication/pdf1927084${dspace.ui.url}/bitstream/11600/49371/1/WOS000386837800001.pdf6ab91645ed46186bfe2caca33a3599f1MD51open accessTEXTWOS000386837800001.pdf.txtWOS000386837800001.pdf.txtExtracted texttext/plain57112${dspace.ui.url}/bitstream/11600/49371/8/WOS000386837800001.pdf.txtff5f4457b1eb2bec3b13c872d22363afMD58open accessTHUMBNAILWOS000386837800001.pdf.jpgWOS000386837800001.pdf.jpgIM Thumbnailimage/jpeg6645${dspace.ui.url}/bitstream/11600/49371/10/WOS000386837800001.pdf.jpg6cb3d65d22a3aebeeffe28837df5d020MD510open access11600/493712023-06-05 19:27:01.452open accessoai:repositorio.unifesp.br:11600/49371Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:27:01Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
title |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
spellingShingle |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry Steinhorst Alcantara, Greyce Kelly Solid-Phase Extraction Hplc-Uv Antiepileptic Drugs Organic-Compounds Human Urine Performance Water Samples Zonisamide Serum |
title_short |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
title_full |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
title_fullStr |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
title_full_unstemmed |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
title_sort |
Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry |
author |
Steinhorst Alcantara, Greyce Kelly |
author_facet |
Steinhorst Alcantara, Greyce Kelly Calixto, Leandro Augusto [UNIFESP] Beraldo de Moraes, Luiz Alberto Costa Queiroz, Regina Helena Moraes de Oliveira, Anderson Rodrigo de Gaitani, Cristiane Masetto |
author_role |
author |
author2 |
Calixto, Leandro Augusto [UNIFESP] Beraldo de Moraes, Luiz Alberto Costa Queiroz, Regina Helena Moraes de Oliveira, Anderson Rodrigo de Gaitani, Cristiane Masetto |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Steinhorst Alcantara, Greyce Kelly Calixto, Leandro Augusto [UNIFESP] Beraldo de Moraes, Luiz Alberto Costa Queiroz, Regina Helena Moraes de Oliveira, Anderson Rodrigo de Gaitani, Cristiane Masetto |
dc.subject.eng.fl_str_mv |
Solid-Phase Extraction Hplc-Uv Antiepileptic Drugs Organic-Compounds Human Urine Performance Water Samples Zonisamide Serum |
topic |
Solid-Phase Extraction Hplc-Uv Antiepileptic Drugs Organic-Compounds Human Urine Performance Water Samples Zonisamide Serum |
description |
Levetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes. The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon (R) (10 kDa porous size) without organic solvent consumption was developed. GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas. DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time. Under chosen parameters (extraction solvent: chloroform, 130 mu L |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2019-01-21T10:29:45Z |
dc.date.available.fl_str_mv |
2019-01-21T10:29:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal Of Analytical Methods In Chemistry. London, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/49371 http://dx.doi.org/10.1155/2016/5976324 |
dc.identifier.issn.none.fl_str_mv |
2090-8865 |
dc.identifier.file.none.fl_str_mv |
WOS000386837800001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1155/2016/5976324 |
dc.identifier.wos.none.fl_str_mv |
WOS:000386837800001 |
identifier_str_mv |
Journal Of Analytical Methods In Chemistry. London, 2016. 2090-8865 WOS000386837800001.pdf 10.1155/2016/5976324 WOS:000386837800001 |
url |
http://repositorio.unifesp.br/handle/11600/49371 http://dx.doi.org/10.1155/2016/5976324 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Journal Of Analytical Methods In Chemistry |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Univ Sao Paulo, Inst Psiquiatria |
publisher.none.fl_str_mv |
Univ Sao Paulo, Inst Psiquiatria |
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Repositório Institucional da UNIFESP |
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