Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1095/biolreprod.107.067413 https://repositorio.unifesp.br/handle/11600/30862 |
Resumo: | The efferent ductules express the highest amount of estrogen receptors ESR1 (ERalpha) and ESR2 (ERbeta) within the male reproductive tract. Treatment of rats with the antiestrogen fulvestrant (ICI 182,780) causes inhibition of fluid reabsorption in the efferent ductules, leading to seminiferous tubule atrophy and infertility. To provide a more comprehensive knowledge about the molecular targets for estrogen in the rat efferent ductules, we investigated the effects of ICI 182,780 treatment on gene expression using a microarray approach. Treatment with ICI 182,780 increased or reduced at least 2-fold the expression of 263 and 98 genes, respectively. Not surprisingly, several genes that encode ion channels and macromolecule transporters were affected. Interestingly, treatment with ICI 182,780 markedly altered the expression of genes related to extracellular matrix organization. Matrix metalloproteinase 7 (Mmp7), osteopontin (Spp1), and neuronal pentraxin 1 (Nptx1) were among the most altered genes in this category. Upregulation of Mmp7 and Spp1 and downregulation of Nptx1 were validated by Northern blot. Increase in Mmp7 expression was further confirmed by immunohistochemistry and probably accounted for the decrease in collagen content observed in the efferent ductules of ICI 182,780-treated animals. Downregulation of Nptx1 probably contributed to the extracellular matrix changes and decreased amyloid deposition in the efferent ductules of ICI 182,780-treated animals. Identification of new molecular targets for estrogen action may help elucidate the regulatory role of this hormone in the male reproductive tract. |
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Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductulesEfferent ductulesEstradiolGene regulationMale reproductive tractThe efferent ductules express the highest amount of estrogen receptors ESR1 (ERalpha) and ESR2 (ERbeta) within the male reproductive tract. Treatment of rats with the antiestrogen fulvestrant (ICI 182,780) causes inhibition of fluid reabsorption in the efferent ductules, leading to seminiferous tubule atrophy and infertility. To provide a more comprehensive knowledge about the molecular targets for estrogen in the rat efferent ductules, we investigated the effects of ICI 182,780 treatment on gene expression using a microarray approach. Treatment with ICI 182,780 increased or reduced at least 2-fold the expression of 263 and 98 genes, respectively. Not surprisingly, several genes that encode ion channels and macromolecule transporters were affected. Interestingly, treatment with ICI 182,780 markedly altered the expression of genes related to extracellular matrix organization. Matrix metalloproteinase 7 (Mmp7), osteopontin (Spp1), and neuronal pentraxin 1 (Nptx1) were among the most altered genes in this category. Upregulation of Mmp7 and Spp1 and downregulation of Nptx1 were validated by Northern blot. Increase in Mmp7 expression was further confirmed by immunohistochemistry and probably accounted for the decrease in collagen content observed in the efferent ductules of ICI 182,780-treated animals. Downregulation of Nptx1 probably contributed to the extracellular matrix changes and decreased amyloid deposition in the efferent ductules of ICI 182,780-treated animals. Identification of new molecular targets for estrogen action may help elucidate the regulatory role of this hormone in the male reproductive tract.Universidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Pharmacol, Sect Expt Endocrinol, Escola Paulista Med,INFAR, BR-04044020 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP: 2003/10132-0Soc Study ReproductionUniversidade Federal de São Paulo (UNIFESP)Yasuhara, Fabiana [UNIFESP]Gomes, Gisele Renata de Oliveira [UNIFESP]Siu, Erica Rosanna [UNIFESP]Suenaga, Claudia Igushi [UNIFESP]Marostica, Elisabeth [UNIFESP]Porto, Catarina Segreti [UNIFESP]Lazari, Maria de Fatima Magalhaes [UNIFESP]2016-01-24T13:51:38Z2016-01-24T13:51:38Z2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion432-441https://dx.doi.org/10.1095/biolreprod.107.067413Biology of Reproduction. Madison: Soc Study Reproduction, v. 79, n. 3, p. 432-441, 2008.10.1095/biolreprod.107.0674130006-3363https://repositorio.unifesp.br/handle/11600/30862WOS:000258580000005engBiology of Reproductioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-01-25T19:37:11Zoai:repositorio.unifesp.br/:11600/30862Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-01-25T19:37:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
title |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
spellingShingle |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules Yasuhara, Fabiana [UNIFESP] Efferent ductules Estradiol Gene regulation Male reproductive tract |
title_short |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
title_full |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
title_fullStr |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
title_full_unstemmed |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
title_sort |
Effects of the antiestrogen fulvestrant (ICI 182,780) on gene expression of the rat efferent ductules |
author |
Yasuhara, Fabiana [UNIFESP] |
author_facet |
Yasuhara, Fabiana [UNIFESP] Gomes, Gisele Renata de Oliveira [UNIFESP] Siu, Erica Rosanna [UNIFESP] Suenaga, Claudia Igushi [UNIFESP] Marostica, Elisabeth [UNIFESP] Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
author_role |
author |
author2 |
Gomes, Gisele Renata de Oliveira [UNIFESP] Siu, Erica Rosanna [UNIFESP] Suenaga, Claudia Igushi [UNIFESP] Marostica, Elisabeth [UNIFESP] Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Yasuhara, Fabiana [UNIFESP] Gomes, Gisele Renata de Oliveira [UNIFESP] Siu, Erica Rosanna [UNIFESP] Suenaga, Claudia Igushi [UNIFESP] Marostica, Elisabeth [UNIFESP] Porto, Catarina Segreti [UNIFESP] Lazari, Maria de Fatima Magalhaes [UNIFESP] |
dc.subject.por.fl_str_mv |
Efferent ductules Estradiol Gene regulation Male reproductive tract |
topic |
Efferent ductules Estradiol Gene regulation Male reproductive tract |
description |
The efferent ductules express the highest amount of estrogen receptors ESR1 (ERalpha) and ESR2 (ERbeta) within the male reproductive tract. Treatment of rats with the antiestrogen fulvestrant (ICI 182,780) causes inhibition of fluid reabsorption in the efferent ductules, leading to seminiferous tubule atrophy and infertility. To provide a more comprehensive knowledge about the molecular targets for estrogen in the rat efferent ductules, we investigated the effects of ICI 182,780 treatment on gene expression using a microarray approach. Treatment with ICI 182,780 increased or reduced at least 2-fold the expression of 263 and 98 genes, respectively. Not surprisingly, several genes that encode ion channels and macromolecule transporters were affected. Interestingly, treatment with ICI 182,780 markedly altered the expression of genes related to extracellular matrix organization. Matrix metalloproteinase 7 (Mmp7), osteopontin (Spp1), and neuronal pentraxin 1 (Nptx1) were among the most altered genes in this category. Upregulation of Mmp7 and Spp1 and downregulation of Nptx1 were validated by Northern blot. Increase in Mmp7 expression was further confirmed by immunohistochemistry and probably accounted for the decrease in collagen content observed in the efferent ductules of ICI 182,780-treated animals. Downregulation of Nptx1 probably contributed to the extracellular matrix changes and decreased amyloid deposition in the efferent ductules of ICI 182,780-treated animals. Identification of new molecular targets for estrogen action may help elucidate the regulatory role of this hormone in the male reproductive tract. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 2016-01-24T13:51:38Z 2016-01-24T13:51:38Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1095/biolreprod.107.067413 Biology of Reproduction. Madison: Soc Study Reproduction, v. 79, n. 3, p. 432-441, 2008. 10.1095/biolreprod.107.067413 0006-3363 https://repositorio.unifesp.br/handle/11600/30862 WOS:000258580000005 |
url |
https://dx.doi.org/10.1095/biolreprod.107.067413 https://repositorio.unifesp.br/handle/11600/30862 |
identifier_str_mv |
Biology of Reproduction. Madison: Soc Study Reproduction, v. 79, n. 3, p. 432-441, 2008. 10.1095/biolreprod.107.067413 0006-3363 WOS:000258580000005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biology of Reproduction |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
432-441 |
dc.publisher.none.fl_str_mv |
Soc Study Reproduction |
publisher.none.fl_str_mv |
Soc Study Reproduction |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268357264801792 |