Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain

Detalhes bibliográficos
Autor(a) principal: Lima, Fabio Mitsuo [UNIFESP]
Data de Publicação: 2013
Outros Autores: Souza, Renata Torres [UNIFESP], Santori, Fabio Rinaldo, Santos, Michele Fernandes [UNIFESP], Cortez, Danielle Rodrigues [UNIFESP], Barros, Roberto Moraes [UNIFESP], Cano, Maria Isabel, Silva Valadares, Helder Magno, Macedo, Andrea Mara, Mortara, Renato Arruda [UNIFESP], Silveira, Jose Franco da [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0063738
http://repositorio.unifesp.br/handle/11600/36335
Resumo: Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure.
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spelling Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G StrainTrypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilNYU, Sch Med, Ctr Canc, Skirball Inst Biomol Med, New York, NY USAUniv Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, BrazilUniv Fed Sao Joao del Rey, Divinopolis, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Public Library ScienceUniversidade Federal de São Paulo (UNIFESP)NYUUniversidade de São Paulo (USP)Universidade Federal de Minas Gerais (UFMG)Univ Fed Sao Joao del ReyLima, Fabio Mitsuo [UNIFESP]Souza, Renata Torres [UNIFESP]Santori, Fabio RinaldoSantos, Michele Fernandes [UNIFESP]Cortez, Danielle Rodrigues [UNIFESP]Barros, Roberto Moraes [UNIFESP]Cano, Maria IsabelSilva Valadares, Helder MagnoMacedo, Andrea MaraMortara, Renato Arruda [UNIFESP]Silveira, Jose Franco da [UNIFESP]2016-01-24T14:31:45Z2016-01-24T14:31:45Z2013-05-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13application/pdfhttp://dx.doi.org/10.1371/journal.pone.0063738Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013.10.1371/journal.pone.0063738WOS000319654700183.pdf1932-6203http://repositorio.unifesp.br/handle/11600/36335WOS:000319654700183engPlos Oneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T07:58:59Zoai:repositorio.unifesp.br/:11600/36335Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T07:58:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
title Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
spellingShingle Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
Lima, Fabio Mitsuo [UNIFESP]
title_short Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
title_full Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
title_fullStr Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
title_full_unstemmed Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
title_sort Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
author Lima, Fabio Mitsuo [UNIFESP]
author_facet Lima, Fabio Mitsuo [UNIFESP]
Souza, Renata Torres [UNIFESP]
Santori, Fabio Rinaldo
Santos, Michele Fernandes [UNIFESP]
Cortez, Danielle Rodrigues [UNIFESP]
Barros, Roberto Moraes [UNIFESP]
Cano, Maria Isabel
Silva Valadares, Helder Magno
Macedo, Andrea Mara
Mortara, Renato Arruda [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
author_role author
author2 Souza, Renata Torres [UNIFESP]
Santori, Fabio Rinaldo
Santos, Michele Fernandes [UNIFESP]
Cortez, Danielle Rodrigues [UNIFESP]
Barros, Roberto Moraes [UNIFESP]
Cano, Maria Isabel
Silva Valadares, Helder Magno
Macedo, Andrea Mara
Mortara, Renato Arruda [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
NYU
Universidade de São Paulo (USP)
Universidade Federal de Minas Gerais (UFMG)
Univ Fed Sao Joao del Rey
dc.contributor.author.fl_str_mv Lima, Fabio Mitsuo [UNIFESP]
Souza, Renata Torres [UNIFESP]
Santori, Fabio Rinaldo
Santos, Michele Fernandes [UNIFESP]
Cortez, Danielle Rodrigues [UNIFESP]
Barros, Roberto Moraes [UNIFESP]
Cano, Maria Isabel
Silva Valadares, Helder Magno
Macedo, Andrea Mara
Mortara, Renato Arruda [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
description Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure.
publishDate 2013
dc.date.none.fl_str_mv 2013-05-07
2016-01-24T14:31:45Z
2016-01-24T14:31:45Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0063738
Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013.
10.1371/journal.pone.0063738
WOS000319654700183.pdf
1932-6203
http://repositorio.unifesp.br/handle/11600/36335
WOS:000319654700183
url http://dx.doi.org/10.1371/journal.pone.0063738
http://repositorio.unifesp.br/handle/11600/36335
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013.
10.1371/journal.pone.0063738
WOS000319654700183.pdf
1932-6203
WOS:000319654700183
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos One
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 13
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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