Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0063738 http://repositorio.unifesp.br/handle/11600/36335 |
Resumo: | Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure. |
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Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G StrainTrypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilNYU, Sch Med, Ctr Canc, Skirball Inst Biomol Med, New York, NY USAUniv Estadual Paulista, Inst Biociencias, Dept Genet, Botucatu, SP, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, BrazilUniv Fed Sao Joao del Rey, Divinopolis, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Public Library ScienceUniversidade Federal de São Paulo (UNIFESP)NYUUniversidade de São Paulo (USP)Universidade Federal de Minas Gerais (UFMG)Univ Fed Sao Joao del ReyLima, Fabio Mitsuo [UNIFESP]Souza, Renata Torres [UNIFESP]Santori, Fabio RinaldoSantos, Michele Fernandes [UNIFESP]Cortez, Danielle Rodrigues [UNIFESP]Barros, Roberto Moraes [UNIFESP]Cano, Maria IsabelSilva Valadares, Helder MagnoMacedo, Andrea MaraMortara, Renato Arruda [UNIFESP]Silveira, Jose Franco da [UNIFESP]2016-01-24T14:31:45Z2016-01-24T14:31:45Z2013-05-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion13application/pdfhttp://dx.doi.org/10.1371/journal.pone.0063738Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013.10.1371/journal.pone.0063738WOS000319654700183.pdf1932-6203http://repositorio.unifesp.br/handle/11600/36335WOS:000319654700183engPlos Oneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T07:58:59Zoai:repositorio.unifesp.br/:11600/36335Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T07:58:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
title |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
spellingShingle |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain Lima, Fabio Mitsuo [UNIFESP] |
title_short |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
title_full |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
title_fullStr |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
title_full_unstemmed |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
title_sort |
Interclonal Variations in the Molecular Karyotype of Trypanosoma cruzi: Chromosome Rearrangements in a Single Cell-Derived Clone of the G Strain |
author |
Lima, Fabio Mitsuo [UNIFESP] |
author_facet |
Lima, Fabio Mitsuo [UNIFESP] Souza, Renata Torres [UNIFESP] Santori, Fabio Rinaldo Santos, Michele Fernandes [UNIFESP] Cortez, Danielle Rodrigues [UNIFESP] Barros, Roberto Moraes [UNIFESP] Cano, Maria Isabel Silva Valadares, Helder Magno Macedo, Andrea Mara Mortara, Renato Arruda [UNIFESP] Silveira, Jose Franco da [UNIFESP] |
author_role |
author |
author2 |
Souza, Renata Torres [UNIFESP] Santori, Fabio Rinaldo Santos, Michele Fernandes [UNIFESP] Cortez, Danielle Rodrigues [UNIFESP] Barros, Roberto Moraes [UNIFESP] Cano, Maria Isabel Silva Valadares, Helder Magno Macedo, Andrea Mara Mortara, Renato Arruda [UNIFESP] Silveira, Jose Franco da [UNIFESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) NYU Universidade de São Paulo (USP) Universidade Federal de Minas Gerais (UFMG) Univ Fed Sao Joao del Rey |
dc.contributor.author.fl_str_mv |
Lima, Fabio Mitsuo [UNIFESP] Souza, Renata Torres [UNIFESP] Santori, Fabio Rinaldo Santos, Michele Fernandes [UNIFESP] Cortez, Danielle Rodrigues [UNIFESP] Barros, Roberto Moraes [UNIFESP] Cano, Maria Isabel Silva Valadares, Helder Magno Macedo, Andrea Mara Mortara, Renato Arruda [UNIFESP] Silveira, Jose Franco da [UNIFESP] |
description |
Trypanosoma cruzi comprises a pool of populations which are genetically diverse in terms of DNA content, growth and infectivity. Inter- and intra-strain karyotype heterogeneities have been reported, suggesting that chromosomal rearrangements occurred during the evolution of this parasite. Clone D11 is a single-cell-derived clone of the T. cruzi G strain selected by the minimal dilution method and by infecting Vero cells with metacyclic trypomastigotes. Here we report that the karyotype of clone D11 differs from that of the G strain in both number and size of chromosomal bands. Large chromosomal rearrangement was observed in the chromosomes carrying the tubulin loci. However, most of the chromosome length polymorphisms were of small amplitude, and the absence of one band in clone D11 in relation to its reference position in the G strain could be correlated to the presence of a novel band migrating above or below this position. Despite the presence of chromosomal polymorphism, large syntenic groups were conserved between the isolates. the appearance of new chromosomal bands in clone D11 could be explained by chromosome fusion followed by a chromosome break or interchromosomal exchange of large DNA segments. Our results also suggest that telomeric regions are involved in this process. the variant represented by clone D11 could have been induced by the stress of the cloning procedure or could, as has been suggested for Leishmania infantum, have emerged from a multiclonal, mosaic parasite population submitted to frequent DNA amplification/deletion events, leading to a 'mosaic' structure with different individuals having differently sized versions of the same chromosomes. If this is the case, the variant represented by clone D11 would be better adapted to survive the stress induced by cloning, which includes intracellular development in the mammalian cell. Karyotype polymorphism could be part of the T. cruzi arsenal for responding to environmental pressure. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-07 2016-01-24T14:31:45Z 2016-01-24T14:31:45Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0063738 Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013. 10.1371/journal.pone.0063738 WOS000319654700183.pdf 1932-6203 http://repositorio.unifesp.br/handle/11600/36335 WOS:000319654700183 |
url |
http://dx.doi.org/10.1371/journal.pone.0063738 http://repositorio.unifesp.br/handle/11600/36335 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 5, 13 p., 2013. 10.1371/journal.pone.0063738 WOS000319654700183.pdf 1932-6203 WOS:000319654700183 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268371989954560 |