Gene expression analysis of Paracoccidioides brasiliensis transition from conidium to yeast cell

• Autor(a) principal: Garcia, Ana M.
• Data de Publicação: 2010
• Outros Autores: Hernandez, Orville, Aristizabal, Beatriz H., Bernardes, Luciano A. de Souza, Puccia, Rosana [UNIFESP], Naranjo, Tony W., Goldman, Gustavo H., Goldman, Maria H., Cano, Luz E., Restrepo, Angela, Mcewen, Juan G.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3109/13693780903055673; http://repositorio.unifesp.br/handle/11600/32203
• topic: Paracoccidioides brasiliensis; conidia; yeast cells; transition; genes; EST-Library

Paracoccidioides brasiliensis infectious process relies on the initial expression of virulence faactors that are assumed to be controlled by molecular mechanisms through which the conidia and/or mycelial fragments convert to yeast cells. in order to analyze the profile of the thermally-induced dimorphic gene expression, 48 h C-L transition cultures which had been incubated at 36 degrees C were studied. By this time approximately 50% of the conidial population had already reverted to yeast form cells. At this transition time, an EST-Orestes library was constructed and characterized. As a result, 79 sequences were obtained, of which 39 (49.4%) had not been described previously in other libraries of this fungus and which could represent novel exclusive C-Y transition genes. Two of these sequences are, among others, cholestanol delta-isomerase, and electron transfer flavoprotein-ubiquinoneoxidoreductase (ETF-QO). the other 40 (50.6%) sequences were shared with Mycelia (M), Yeast (Y) or Mycelia to yest transition (M-Y) libraries. An important component of this group of sequences is a putative response regulator receiver SKN7, a protein of high importance in stress adaptation and a regulator of virulence in some bacteria and fungi. This is the first report identifying genes expressed during the C-Y transition process, the initial step required to understand the natural history of P brasiliensis conidia induced infection.