Tissue Microarray Analysis Applied to Bone Diagenesis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55269 http://dx.doi.org/10.1038/srep39987 |
Resumo: | Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7% nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered. |
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Mello, Rafael Barrios [UNIFESP]Regis Silva, Maria Regina [UNIFESP]Seixas Alves, Maria Teresa [UNIFESP]Evison, Martin PaulGuimaraes, Marco AurelioFrancisco, Rafaella ArrabacaAstolphi, Rafael Dias [UNIFESP]Miazato Iwamura, Edna Sadayo [UNIFESP]2020-07-17T14:03:16Z2020-07-17T14:03:16Z2017Scientific Reports. London, v. 7, p. -, 2017.2045-2322https://repositorio.unifesp.br/handle/11600/55269http://dx.doi.org/10.1038/srep39987WOS000391268900001.pdf10.1038/srep39987WOS:000391268900001Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7% nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered.CODERPFAPESPCNPqUniv Fed Sao Paulo, Dept Pathol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilNorthumbria Univ, Fac Hlth & Life Sci, Newcastle Upon Tyne NE1 8ST, Tyne & Wear, EnglandUniv Sao Paulo, Ribeirao Preto Med Sch, Ctr Legal Med, BR-14049900 Ribeirao Preto, BrazilUniv Fed Sao Paulo, Dept Pathol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilFAPESP: 2012/12344-3FAPESP: 2012/24918-4FAPESP: 2013/17901-0CNPq: 232415/2014-5Web of Science-engNature Publishing GroupScientific ReportsTissue Microarray Analysis Applied to Bone Diagenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon7info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000391268900001.pdfapplication/pdf1289078${dspace.ui.url}/bitstream/11600/55269/1/WOS000391268900001.pdf6e1eb5a6a8b405ec097e04f5afb0c4ddMD51open accessTEXTWOS000391268900001.pdf.txtWOS000391268900001.pdf.txtExtracted texttext/plain61329${dspace.ui.url}/bitstream/11600/55269/2/WOS000391268900001.pdf.txt5e24cdf04885390d3c5dc4c909228f9bMD52open accessTHUMBNAILWOS000391268900001.pdf.jpgWOS000391268900001.pdf.jpgIM Thumbnailimage/jpeg7332${dspace.ui.url}/bitstream/11600/55269/4/WOS000391268900001.pdf.jpgf616e146c5ca04aedd2b08e4164248a6MD54open access11600/552692022-08-01 04:22:20.508open accessoai:repositorio.unifesp.br:11600/55269Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-08-01T07:22:20Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Tissue Microarray Analysis Applied to Bone Diagenesis |
title |
Tissue Microarray Analysis Applied to Bone Diagenesis |
spellingShingle |
Tissue Microarray Analysis Applied to Bone Diagenesis Mello, Rafael Barrios [UNIFESP] |
title_short |
Tissue Microarray Analysis Applied to Bone Diagenesis |
title_full |
Tissue Microarray Analysis Applied to Bone Diagenesis |
title_fullStr |
Tissue Microarray Analysis Applied to Bone Diagenesis |
title_full_unstemmed |
Tissue Microarray Analysis Applied to Bone Diagenesis |
title_sort |
Tissue Microarray Analysis Applied to Bone Diagenesis |
author |
Mello, Rafael Barrios [UNIFESP] |
author_facet |
Mello, Rafael Barrios [UNIFESP] Regis Silva, Maria Regina [UNIFESP] Seixas Alves, Maria Teresa [UNIFESP] Evison, Martin Paul Guimaraes, Marco Aurelio Francisco, Rafaella Arrabaca Astolphi, Rafael Dias [UNIFESP] Miazato Iwamura, Edna Sadayo [UNIFESP] |
author_role |
author |
author2 |
Regis Silva, Maria Regina [UNIFESP] Seixas Alves, Maria Teresa [UNIFESP] Evison, Martin Paul Guimaraes, Marco Aurelio Francisco, Rafaella Arrabaca Astolphi, Rafael Dias [UNIFESP] Miazato Iwamura, Edna Sadayo [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mello, Rafael Barrios [UNIFESP] Regis Silva, Maria Regina [UNIFESP] Seixas Alves, Maria Teresa [UNIFESP] Evison, Martin Paul Guimaraes, Marco Aurelio Francisco, Rafaella Arrabaca Astolphi, Rafael Dias [UNIFESP] Miazato Iwamura, Edna Sadayo [UNIFESP] |
description |
Taphonomic processes affecting bone post mortem are important in forensic, archaeological and palaeontological investigations. In this study, the application of tissue microarray (TMA) analysis to a sample of femoral bone specimens from 20 exhumed individuals of known period of burial and age at death is described. TMA allows multiplexing of subsamples, permitting standardized comparative analysis of adjacent sections in 3-D and of representative cross-sections of a large number of specimens. Standard hematoxylin and eosin, periodic acid-Schiff and silver methenamine, and picrosirius red staining, and CD31 and CD34 immunohistochemistry were applied to TMA sections. Osteocyte and osteocyte lacuna counts, percent bone matrix loss, and fungal spheroid element counts could be measured and collagen fibre bundles observed in all specimens. Decalcification with 7% nitric acid proceeded more rapidly than with 0.5 M EDTA and may offer better preservation of histological and cellular structure. No endothelial cells could be detected using CD31 and CD34 immunohistochemistry. Correlation between osteocytes per lacuna and age at death may reflect reported age-related responses to microdamage. Methodological limitations and caveats, and results of the TMA analysis of post mortem diagenesis in bone are discussed, and implications for DNA survival and recovery considered. |
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2017 |
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2017 |
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2020-07-17T14:03:16Z |
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2020-07-17T14:03:16Z |
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dc.identifier.citation.fl_str_mv |
Scientific Reports. London, v. 7, p. -, 2017. |
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https://repositorio.unifesp.br/handle/11600/55269 http://dx.doi.org/10.1038/srep39987 |
dc.identifier.issn.none.fl_str_mv |
2045-2322 |
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WOS000391268900001.pdf |
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10.1038/srep39987 |
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WOS:000391268900001 |
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Scientific Reports. London, v. 7, p. -, 2017. 2045-2322 WOS000391268900001.pdf 10.1038/srep39987 WOS:000391268900001 |
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https://repositorio.unifesp.br/handle/11600/55269 http://dx.doi.org/10.1038/srep39987 |
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