Estudo da morfologia renal após a oclusão da aorta abdominal infra-renal em ratos

• Autor(a) principal: Feitosa, Eliane Alencar do Nascimento [UNIFESP]
• Data de Publicação: 2005
• Outros Autores: Taha, Murched Omar [UNIFESP], Fagundes, Djalma José [UNIFESP], Takiya, Cristina Maeda, Cardoso, Lúcio Ronaldo, Campos, Doris Moura
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0100-69912005000400004; http://repositorio.unifesp.br/handle/11600/2612
• topic: Morphology; Kidney; Aorta abdominal; Rats, Wistar; Morfologia; Rim; Aorta abdominal; Ratos, Wistar

BACKGROUND: The aim of this study was to investigate the influence of infrarenal corss-clamping in the renal tissue. METHODS: Groups of male Wistar rats (N = 60) were subjected or not to infrarenal aortic cross-clamping following or not by reperfusion according to each group: IA (sham-30 min-n = 10); IB (sham-60min-n = 10); IIA (30min of ischaemia-n = 10); IIB (60min of ischaemia-n =10); IIIA (30min of ischaemia + 30min of reperfusion-n = 10); IIIB (60min of ischaemia + 60min of reperfusionn = 10). All animals were submitted to nephrectomy. The histological analyses (descriptive and morphometry) were accomplished through conventional methodology (paraffin embedding and hematoxilylin and eosin staining). Semiquantitative analyses of tubular damage (modified structure) and intersticial edema were carried out according to an index of intensity of injury. Data was validated by applying statistical tests: Mann-Whitney, Kruskal-Wallis, Multiple Comparisons. Significance was accepted at p < 0.001. RESULTS: Occurrence of signicant tubular and intersticial damages in group III was observed. CONCLUSION: Occlusion of the infrarenal aorta in rats can lead to renal morphological effects, especially in reperfusion phase.