Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502004000500005 http://repositorio.unifesp.br/handle/11600/2227 |
Resumo: | PURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of jejunum were processed histologically for morphometric measure of the total thickness of the jejujum's wall in comparison to the thickness of the mucosa + submucosa layer. RESULTS: In blood culture, there was growth of Pseudomonas aeruginosa and Escherichia coli in 90% and 52,5% of the animals, respectively. In the peritoneal culture, there was growth of P aeruginosa, E.coli and Klebsiella sp in 87,5%, 85% and 5% of the animals. About the histological analysis and the thickness of the mucosa + submucosa's layer, there was not significant alteration. CONCLUSION: Acute sepsis did not develop any thickness alteration in the small intestine's mucosa layer. Bacterial translocation can not be a direct consequence produced by mucosal intestinal injury. |
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Atrofia mucosa/translocação bacteriana na sepse experimental em ratos WistarMucosal atrophy/bacterial translocation in experimental sepsis in Wistar ratsAtrophyMucosalIntestineTranslocationAtrofiaMucosaIntestinoTranslocaçãoPURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of jejunum were processed histologically for morphometric measure of the total thickness of the jejujum's wall in comparison to the thickness of the mucosa + submucosa layer. RESULTS: In blood culture, there was growth of Pseudomonas aeruginosa and Escherichia coli in 90% and 52,5% of the animals, respectively. In the peritoneal culture, there was growth of P aeruginosa, E.coli and Klebsiella sp in 87,5%, 85% and 5% of the animals. About the histological analysis and the thickness of the mucosa + submucosa's layer, there was not significant alteration. CONCLUSION: Acute sepsis did not develop any thickness alteration in the small intestine's mucosa layer. Bacterial translocation can not be a direct consequence produced by mucosal intestinal injury.OBJETIVO: Avalizar a relação entre lesão mucosa e translocação bacteriana. MÉTODO: Utilizou-se 50 ratos distribuídos em 5 grupos: 1.Controle: injeção de inóculo padrão de Pseudomonas aeruginosa, 2.Dreno: injeção do inóculo padrão e drenagem da cavidade abdominal, após 6 horas, 3.Lavado: injeção do inóculo padrão e lavagem da cavidade abdominal, após 6 horas, 4.Lavado + dreno: injeção do inóculo padrão e após 6 horas, drenagem e lavagem da cavidade, 5.Normal: avaliação histológica da parede intestinal normal. Após o óbito, realizou-se hemocultura e cultura peritoneal. Realizou-se medida da espessura total da parede do jejuno e da camada mucosa em vilosidades seccionadas de forma longitudinal. RESULTADOS: Na hemocultura, houve crescimento de Pseudomonas aeruginosa e Escherichia coli em 90% e 52,5% dos animais. Na cultura peritoneal, houve crescimento de P. aeruginosa, E. coli e Klebsiella sp em 87,5%, 85% e 5% dos animais. Quanto a altura da camada mucosa e da parede intestinal, não houve alteração estatisticamente significativa entre os 5 grupos. CONCLUSÃO: A sepse aguda não causou alteração na camada mucosa do intestino delgado e a translocação ocorrida não pode ser considerada como decorrente de uma lesão da mucosa intestinal.Universidade Federal de São Paulo (UNIFESP)Hospital São PauloUniversidade Federal de Santa Catarina Hospital UniversitárioHospital Regional de São JoséUniversidade Federal de Santa CatarinaUNIFESPHospital São PauloSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Universidade Federal de Santa Catarina Hospital UniversitárioHospital Regional de São JoséUniversidade Federal de Santa CatarinaD'acampora, Armando José [UNIFESP]Ortellado, Daniel Knabben [UNIFESP]Carvalho, Roberta O. M.Serafim, João Daniel MayFarias, Débora Cadore DeTramonte, Ricardo2015-06-14T13:31:16Z2015-06-14T13:31:16Z2004-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion478-486application/pdfhttp://dx.doi.org/10.1590/S0102-86502004000500005Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, n. 5, p. 478-486, 2004.10.1590/S0102-86502004000500005S0102-86502004000500005.pdf0102-8650S0102-86502004000500005http://repositorio.unifesp.br/handle/11600/2227porActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-28T07:46:04Zoai:repositorio.unifesp.br/:11600/2227Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-28T07:46:04Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar Mucosal atrophy/bacterial translocation in experimental sepsis in Wistar rats |
title |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
spellingShingle |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar D'acampora, Armando José [UNIFESP] Atrophy Mucosal Intestine Translocation Atrofia Mucosa Intestino Translocação |
title_short |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
title_full |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
title_fullStr |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
title_full_unstemmed |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
title_sort |
Atrofia mucosa/translocação bacteriana na sepse experimental em ratos Wistar |
author |
D'acampora, Armando José [UNIFESP] |
author_facet |
D'acampora, Armando José [UNIFESP] Ortellado, Daniel Knabben [UNIFESP] Carvalho, Roberta O. M. Serafim, João Daniel May Farias, Débora Cadore De Tramonte, Ricardo |
author_role |
author |
author2 |
Ortellado, Daniel Knabben [UNIFESP] Carvalho, Roberta O. M. Serafim, João Daniel May Farias, Débora Cadore De Tramonte, Ricardo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Federal de Santa Catarina Hospital Universitário Hospital Regional de São José Universidade Federal de Santa Catarina |
dc.contributor.author.fl_str_mv |
D'acampora, Armando José [UNIFESP] Ortellado, Daniel Knabben [UNIFESP] Carvalho, Roberta O. M. Serafim, João Daniel May Farias, Débora Cadore De Tramonte, Ricardo |
dc.subject.por.fl_str_mv |
Atrophy Mucosal Intestine Translocation Atrofia Mucosa Intestino Translocação |
topic |
Atrophy Mucosal Intestine Translocation Atrofia Mucosa Intestino Translocação |
description |
PURPOSE: Observe the relation between small intestine's mucosal injury and bacterial translocation. METHODS: 50 adult female rats were distributed in 5 groups: 1. Control: intraperitoneal injection of Pseudomonas aeruginosa, 2. Drainage: intraperitoneal injection of P. aeruginosa and drainage of the abdominal cavity, after 6 hours, 3. Washed: intraperitoneal injection of P. aeruginosa and washing of the abdominal cavity, after 6 hours, 4. Washed + drainage: intraperitoneal injection of P. aeruginosa plus drainage and washing of the cavity, after 6 hours, 5. Normal: evaluation of the normal intestinal wall. After death, blood and peritoneal cultures were performed. Fragments of jejunum were processed histologically for morphometric measure of the total thickness of the jejujum's wall in comparison to the thickness of the mucosa + submucosa layer. RESULTS: In blood culture, there was growth of Pseudomonas aeruginosa and Escherichia coli in 90% and 52,5% of the animals, respectively. In the peritoneal culture, there was growth of P aeruginosa, E.coli and Klebsiella sp in 87,5%, 85% and 5% of the animals. About the histological analysis and the thickness of the mucosa + submucosa's layer, there was not significant alteration. CONCLUSION: Acute sepsis did not develop any thickness alteration in the small intestine's mucosa layer. Bacterial translocation can not be a direct consequence produced by mucosal intestinal injury. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-10-01 2015-06-14T13:31:16Z 2015-06-14T13:31:16Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502004000500005 Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, n. 5, p. 478-486, 2004. 10.1590/S0102-86502004000500005 S0102-86502004000500005.pdf 0102-8650 S0102-86502004000500005 http://repositorio.unifesp.br/handle/11600/2227 |
url |
http://dx.doi.org/10.1590/S0102-86502004000500005 http://repositorio.unifesp.br/handle/11600/2227 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 19, n. 5, p. 478-486, 2004. 10.1590/S0102-86502004000500005 S0102-86502004000500005.pdf 0102-8650 S0102-86502004000500005 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
478-486 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268381347446784 |