Clinical implication of 14-3-3 epsilon expression in gastric cancer

• Autor(a) principal: Leal, Mariana Ferreira [UNIFESP]
• Data de Publicação: 2012
• Outros Autores: Calcagno, Danielle Queiroz [UNIFESP], Demachki, Samia, Assumpcao, Paulo Pimentel, Chammas, Roger, Burbano, Rommel Rodriguez, Smith, Marilia de Arruda Cardoso [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3748/wjg.v18.i13.1531; http://repositorio.unifesp.br/handle/11600/34794
• topic: Gastric cancer; 14-3-3 epsilon; YWHAE; Gene expression; Protein expression

AIM: To evaluate for the first time the protein and mRNA expression of 14-3-3 epsilon in gastric carcinogenesis.METHODS: 14-3-3 epsilon protein expression was determined by western blotting, and mRNA expression was examined by real-time quantitative RT-PCR in gastric tumors and their matched non-neoplastic gastric tissue samples.RESULTS: Authors observed a significant reduction of 14-3-3 epsilon protein expression in gastric cancer (GC) samples compared to their matched non-neoplastic tissue, Reduced levels of 14-3-3 epsilon were also associated with diffuse-type GC and early-onset of this pathology. Our data suggest that reduced 14-3-3 epsilon may have a role in gastric carcinogenesis process.CONCLUSION: Our results reveal that the reduced 14-3-3 epsilon expression in GC and investigation of 14-3-3 epsilon interaction partners may help to elucidate the carcinogenesis process. (C) 2012 Baishideng. All rights reserved.