Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection

Detalhes bibliográficos
Autor(a) principal: Eickhoff, Christopher S
Data de Publicação: 2010
Outros Autores: Giddings, Olivia K, Yoshida, Nobuko [UNIFESP], Hoft, Daniel F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02762010000500015
http://repositorio.unifesp.br/handle/11600/5853
Resumo: The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
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spelling Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infectionTrypanosoma cruzimucosal immunityvaccinesThe potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.Saint Louis University Department of Molecular MicrobiologyUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeSaint Louis University Department of Molecular MicrobiologyUniversidade Federal de São Paulo (UNIFESP)Eickhoff, Christopher SGiddings, Olivia KYoshida, Nobuko [UNIFESP]Hoft, Daniel F2015-06-14T13:41:48Z2015-06-14T13:41:48Z2010-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion687-691application/pdfhttp://dx.doi.org/10.1590/S0074-02762010000500015Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 5, p. 687-691, 2010.10.1590/S0074-02762010000500015S0074-02762010000500015.pdf0074-0276S0074-02762010000500015http://repositorio.unifesp.br/handle/11600/5853engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T01:46:09Zoai:repositorio.unifesp.br/:11600/5853Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T01:46:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
spellingShingle Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
Eickhoff, Christopher S
Trypanosoma cruzi
mucosal immunity
vaccines
title_short Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_full Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_fullStr Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_full_unstemmed Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
title_sort Immune responses to gp82 provide protection against mucosal Trypanosoma cruzi infection
author Eickhoff, Christopher S
author_facet Eickhoff, Christopher S
Giddings, Olivia K
Yoshida, Nobuko [UNIFESP]
Hoft, Daniel F
author_role author
author2 Giddings, Olivia K
Yoshida, Nobuko [UNIFESP]
Hoft, Daniel F
author2_role author
author
author
dc.contributor.none.fl_str_mv Saint Louis University Department of Molecular Microbiology
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Eickhoff, Christopher S
Giddings, Olivia K
Yoshida, Nobuko [UNIFESP]
Hoft, Daniel F
dc.subject.por.fl_str_mv Trypanosoma cruzi
mucosal immunity
vaccines
topic Trypanosoma cruzi
mucosal immunity
vaccines
description The potential use of the Trypanosoma cruzi metacyclic trypomastigote (MT) stage-specific molecule glycoprotein-82 (gp82) as a vaccine target has not been fully explored. We show that the opsonization of T. cruzi MT with gp82-specific antibody prior to mucosal challenge significantly reduces parasite infectivity. In addition, we investigated the immune responses as well as the systemic and mucosal protective immunity induced by intranasal CpG-adjuvanted gp82 vaccination. Spleen cells from mice immunized with CpG-gp82 proliferated and secreted IFN-γ in a dose-dependent manner in response to in vitro stimulation with gp82 and parasite lysate. More importantly, these CpG-gp82-immunized mice were significantly protected from a biologically relevant oral parasite challenge.
publishDate 2010
dc.date.none.fl_str_mv 2010-08-01
2015-06-14T13:41:48Z
2015-06-14T13:41:48Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02762010000500015
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 5, p. 687-691, 2010.
10.1590/S0074-02762010000500015
S0074-02762010000500015.pdf
0074-0276
S0074-02762010000500015
http://repositorio.unifesp.br/handle/11600/5853
url http://dx.doi.org/10.1590/S0074-02762010000500015
http://repositorio.unifesp.br/handle/11600/5853
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 5, p. 687-691, 2010.
10.1590/S0074-02762010000500015
S0074-02762010000500015.pdf
0074-0276
S0074-02762010000500015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 687-691
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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