Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Rittner, Glauce M. G.
Data de Publicação: 2012
Outros Autores: Munoz, Julian E., Marques, Alexandre F., Nosanchuk, Joshua D., Taborda, Carlos Pelleschi [UNIFESP], Travassos, Luiz R. [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pntd.0001519
http://repositorio.unifesp.br/handle/11600/34554
Resumo: Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients.
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spelling Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental ParacoccidioidomycosisParacoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients.Univ São Paulo, Inst Biomed Sci, Dept Microbiol, São Paulo, BrazilAlbert Einstein Coll Med, Dept Med, Bronx, NY 10467 USAAlbert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USAUniv São Paulo, Med Mycol Lab IMTSP & HCFMUSP LIM53, São Paulo, BrazilFed Univ São Paulo UNIFESP, Div Cell Biol, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilFed Univ São Paulo UNIFESP, Div Cell Biol, Dept Microbiol Immunol & Parasitol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 09/15823-7FAPESP: 07/07588-2FAPESP: 06/50634-2FAPESP: 05/02776-0CNPq: 470513/2009-8Public Library ScienceUniversidade de São Paulo (USP)Albert Einstein Coll MedUniversidade Federal de São Paulo (UNIFESP)Rittner, Glauce M. G.Munoz, Julian E.Marques, Alexandre F.Nosanchuk, Joshua D.Taborda, Carlos Pelleschi [UNIFESP]Travassos, Luiz R. [UNIFESP]2016-01-24T14:17:50Z2016-01-24T14:17:50Z2012-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9application/pdfhttp://dx.doi.org/10.1371/journal.pntd.0001519Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012.10.1371/journal.pntd.0001519WOS000300949200031.pdf1935-2735http://repositorio.unifesp.br/handle/11600/34554WOS:000300949200031engPlos Neglected Tropical Diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T23:59:09Zoai:repositorio.unifesp.br/:11600/34554Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T23:59:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
title Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
spellingShingle Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
Rittner, Glauce M. G.
title_short Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
title_full Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
title_fullStr Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
title_full_unstemmed Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
title_sort Therapeutic DNA Vaccine Encoding Peptide P10 against Experimental Paracoccidioidomycosis
author Rittner, Glauce M. G.
author_facet Rittner, Glauce M. G.
Munoz, Julian E.
Marques, Alexandre F.
Nosanchuk, Joshua D.
Taborda, Carlos Pelleschi [UNIFESP]
Travassos, Luiz R. [UNIFESP]
author_role author
author2 Munoz, Julian E.
Marques, Alexandre F.
Nosanchuk, Joshua D.
Taborda, Carlos Pelleschi [UNIFESP]
Travassos, Luiz R. [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Albert Einstein Coll Med
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Rittner, Glauce M. G.
Munoz, Julian E.
Marques, Alexandre F.
Nosanchuk, Joshua D.
Taborda, Carlos Pelleschi [UNIFESP]
Travassos, Luiz R. [UNIFESP]
description Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. the TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. in the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. in fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. the data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients.
publishDate 2012
dc.date.none.fl_str_mv 2012-02-01
2016-01-24T14:17:50Z
2016-01-24T14:17:50Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0001519
Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012.
10.1371/journal.pntd.0001519
WOS000300949200031.pdf
1935-2735
http://repositorio.unifesp.br/handle/11600/34554
WOS:000300949200031
url http://dx.doi.org/10.1371/journal.pntd.0001519
http://repositorio.unifesp.br/handle/11600/34554
identifier_str_mv Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 6, n. 2, 9 p., 2012.
10.1371/journal.pntd.0001519
WOS000300949200031.pdf
1935-2735
WOS:000300949200031
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos Neglected Tropical Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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