Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring
Main Author: | |
---|---|
Publication Date: | 2014 |
Other Authors: | , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNIFESP |
Download full: | http://repositorio.unifesp.br/handle/11600/38461 http://dx.doi.org/10.1371/journal.pone.0113075 |
Summary: | Changes in the maternal environment can induce fetal adaptations that result in the progression of chronic diseases in the offspring. the objective of the present study was to evaluate the effects of maternal chronic sleep restriction on blood pressure, renal function and cardiac baroreflex response on male offspring at adult age. Female 3-month-old Wistar rats were divided in two experimental groups: control (C) and chronic sleep restricted (CSR). Pregnancy was confirmed by vaginal smear. Chronic sleep restricted females were subjected to sleep restriction by the multiple platform technique for 20 h daily, between the 1st and 20th day of pregnancy. After birth, the litters were reduced to 6 rats per mother, and were designated as offspring from control (OC) and offspring from chronic sleep restricted (OCSR). Indirect blood pressure (BPi tail cuff) was measured by plethysmography in male offspring at 3 months old. Following, the renal function and cardiac baroreflex response were analyzed. Values of BPi in OCSR were significantly higher compared to OC [OC: 127 +/- 2.6 (19); OCSR: 144 +/- 2.5 (17) mmHg]. the baroreflex sensitivity to the increase of blood pressure was reduced in OCSR [Slope: OC: -2.6 +/- 0.15 (9); OCRS: -1.6 +/- 0.13 (9)]. Hypothalamic activity of ACE2 was significantly reduced in OCSR compared to OC [OC: 97.4 +/- 15 (18); OSR: 60.2 +/- 3.6 (16) UAF/min/protein mg]. Renal function alteration was noticed by the increase in glomerular filtration rate (GFR) observed in OCSR [OC: 6.4 +/- 0.2 (10); OCSR: 7.4 +/- 0.3 (7)]. Chronic sleep restriction during pregnancy caused in the offspring hypertension, altered cardiac baroreflex response, reduced ACE-2 activity in the hypothalamus and renal alterations. Our data suggest that the reduction of sleeping time along the pregnancy is able to modify maternal homeostasis leading to functional alterations in offspring. |
id |
UFSP_d29023b45e30a8a0986ac5fc95449960 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/38461 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Lima, Ingrid L. B. [UNIFESP]Rodrigues, Aline F. A. C. [UNIFESP]Bergamaschi, Cassia Toledo [UNIFESP]Campos, Ruy Ribeiro [UNIFESP]Hirata, Aparecida Emiko [UNIFESP]Tufik, Sergio [UNIFESP]Xylaras, Beatriz D. P. [UNIFESP]Visniauskas, Bruna [UNIFESP]Chagas, Jair Ribeiro [UNIFESP]Gomes, Guiomar Nascimento [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:38:11Z2016-01-24T14:38:11Z2014-11-18Plos One. San Francisco: Public Library Science, v. 9, n. 11, 7 p., 2014.1932-6203http://repositorio.unifesp.br/handle/11600/38461http://dx.doi.org/10.1371/journal.pone.0113075WOS000347121300074.pdf10.1371/journal.pone.0113075WOS:000347121300074Changes in the maternal environment can induce fetal adaptations that result in the progression of chronic diseases in the offspring. the objective of the present study was to evaluate the effects of maternal chronic sleep restriction on blood pressure, renal function and cardiac baroreflex response on male offspring at adult age. Female 3-month-old Wistar rats were divided in two experimental groups: control (C) and chronic sleep restricted (CSR). Pregnancy was confirmed by vaginal smear. Chronic sleep restricted females were subjected to sleep restriction by the multiple platform technique for 20 h daily, between the 1st and 20th day of pregnancy. After birth, the litters were reduced to 6 rats per mother, and were designated as offspring from control (OC) and offspring from chronic sleep restricted (OCSR). Indirect blood pressure (BPi tail cuff) was measured by plethysmography in male offspring at 3 months old. Following, the renal function and cardiac baroreflex response were analyzed. Values of BPi in OCSR were significantly higher compared to OC [OC: 127 +/- 2.6 (19); OCSR: 144 +/- 2.5 (17) mmHg]. the baroreflex sensitivity to the increase of blood pressure was reduced in OCSR [Slope: OC: -2.6 +/- 0.15 (9); OCRS: -1.6 +/- 0.13 (9)]. Hypothalamic activity of ACE2 was significantly reduced in OCSR compared to OC [OC: 97.4 +/- 15 (18); OSR: 60.2 +/- 3.6 (16) UAF/min/protein mg]. Renal function alteration was noticed by the increase in glomerular filtration rate (GFR) observed in OCSR [OC: 6.4 +/- 0.2 (10); OCSR: 7.4 +/- 0.3 (7)]. Chronic sleep restriction during pregnancy caused in the offspring hypertension, altered cardiac baroreflex response, reduced ACE-2 activity in the hypothalamus and renal alterations. Our data suggest that the reduction of sleeping time along the pregnancy is able to modify maternal homeostasis leading to functional alterations in offspring.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Associacao Fundo de Incentivo a Pesquisa (AFIP)Universidade Federal de São Paulo, Dept Fisiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psicobiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Fisiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psicobiol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilFAPESP: FAPESP-10/51665-4Web of Science7engPublic Library SciencePlos OneChronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspringinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000347121300074.pdfapplication/pdf352013${dspace.ui.url}/bitstream/11600/38461/1/WOS000347121300074.pdf2795a5df609e740ff9098f1623c48ca6MD51open accessTEXTWOS000347121300074.pdf.txtWOS000347121300074.pdf.txtExtracted texttext/plain39638${dspace.ui.url}/bitstream/11600/38461/2/WOS000347121300074.pdf.txt5169c904d6502a271b666caa7dea993dMD52open access11600/384612022-06-02 10:24:17.638open accessoai:repositorio.unifesp.br:11600/38461Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:16:46.728851Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
title |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
spellingShingle |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring Lima, Ingrid L. B. [UNIFESP] |
title_short |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
title_full |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
title_fullStr |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
title_full_unstemmed |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
title_sort |
Chronic Sleep Restriction during Pregnancy - Repercussion on Cardiovascular and Renal Functioning of Male Offspring |
author |
Lima, Ingrid L. B. [UNIFESP] |
author_facet |
Lima, Ingrid L. B. [UNIFESP] Rodrigues, Aline F. A. C. [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] Hirata, Aparecida Emiko [UNIFESP] Tufik, Sergio [UNIFESP] Xylaras, Beatriz D. P. [UNIFESP] Visniauskas, Bruna [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] Gomes, Guiomar Nascimento [UNIFESP] |
author_role |
author |
author2 |
Rodrigues, Aline F. A. C. [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] Hirata, Aparecida Emiko [UNIFESP] Tufik, Sergio [UNIFESP] Xylaras, Beatriz D. P. [UNIFESP] Visniauskas, Bruna [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] Gomes, Guiomar Nascimento [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Lima, Ingrid L. B. [UNIFESP] Rodrigues, Aline F. A. C. [UNIFESP] Bergamaschi, Cassia Toledo [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] Hirata, Aparecida Emiko [UNIFESP] Tufik, Sergio [UNIFESP] Xylaras, Beatriz D. P. [UNIFESP] Visniauskas, Bruna [UNIFESP] Chagas, Jair Ribeiro [UNIFESP] Gomes, Guiomar Nascimento [UNIFESP] |
description |
Changes in the maternal environment can induce fetal adaptations that result in the progression of chronic diseases in the offspring. the objective of the present study was to evaluate the effects of maternal chronic sleep restriction on blood pressure, renal function and cardiac baroreflex response on male offspring at adult age. Female 3-month-old Wistar rats were divided in two experimental groups: control (C) and chronic sleep restricted (CSR). Pregnancy was confirmed by vaginal smear. Chronic sleep restricted females were subjected to sleep restriction by the multiple platform technique for 20 h daily, between the 1st and 20th day of pregnancy. After birth, the litters were reduced to 6 rats per mother, and were designated as offspring from control (OC) and offspring from chronic sleep restricted (OCSR). Indirect blood pressure (BPi tail cuff) was measured by plethysmography in male offspring at 3 months old. Following, the renal function and cardiac baroreflex response were analyzed. Values of BPi in OCSR were significantly higher compared to OC [OC: 127 +/- 2.6 (19); OCSR: 144 +/- 2.5 (17) mmHg]. the baroreflex sensitivity to the increase of blood pressure was reduced in OCSR [Slope: OC: -2.6 +/- 0.15 (9); OCRS: -1.6 +/- 0.13 (9)]. Hypothalamic activity of ACE2 was significantly reduced in OCSR compared to OC [OC: 97.4 +/- 15 (18); OSR: 60.2 +/- 3.6 (16) UAF/min/protein mg]. Renal function alteration was noticed by the increase in glomerular filtration rate (GFR) observed in OCSR [OC: 6.4 +/- 0.2 (10); OCSR: 7.4 +/- 0.3 (7)]. Chronic sleep restriction during pregnancy caused in the offspring hypertension, altered cardiac baroreflex response, reduced ACE-2 activity in the hypothalamus and renal alterations. Our data suggest that the reduction of sleeping time along the pregnancy is able to modify maternal homeostasis leading to functional alterations in offspring. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-18 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:11Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 11, 7 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38461 http://dx.doi.org/10.1371/journal.pone.0113075 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.file.none.fl_str_mv |
WOS000347121300074.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0113075 |
dc.identifier.wos.none.fl_str_mv |
WOS:000347121300074 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 11, 7 p., 2014. 1932-6203 WOS000347121300074.pdf 10.1371/journal.pone.0113075 WOS:000347121300074 |
url |
http://repositorio.unifesp.br/handle/11600/38461 http://dx.doi.org/10.1371/journal.pone.0113075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/38461/1/WOS000347121300074.pdf ${dspace.ui.url}/bitstream/11600/38461/2/WOS000347121300074.pdf.txt |
bitstream.checksum.fl_str_mv |
2795a5df609e740ff9098f1623c48ca6 5169c904d6502a271b666caa7dea993d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1783460271022407680 |