A função renal de ratos espontaneamente hipertensos submetidos ao pneumoperitônio

• Autor(a) principal: Moreira, Márcia Bento [UNIFESP]
• Data de Publicação: 2002
• Outros Autores: Montero, Edna Frasson de Souza [UNIFESP], Fagundes, Djalma José [UNIFESP], Chida, Valeria Vieira, Ramalho, Carlos Eduardo Benetti, Juliano, Yára
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-86502002000300004; http://repositorio.unifesp.br/handle/11600/1417
• topic: Pneumoperitoneum. Artificial; Rats; Inbred SHR; Kidney; Pneumoperitônio artificial; Ratos endogâmicos SHR; Rim

Objective: The aim of this work was to study the renal function of spontaneous hypertensive Wistar rats (SRH) submitted to pneumoperitoneum. Methods: Sixty rats, 30 SHR and 30 EPM-1 Wistar, 4-month-old, weighing around 350 g were utilized. Animals were distributed into two groups: experimental and control, according to the lineage, SHR and EPM-1 Wistar rats, respectively. Each group was divided into two subgroups, with 15 animals each, with pneumoperitoneum pressure established at 4 mmHg and 10 mmHg. The animals were anesthetized and positioned to start the left femoral artery catheterization procedure to measure median arterial pressure and to collect blood sample during the experiment. After antisepsis, a 5 mm length transversal incision in the central abdominal area was performed to introduce a Veress needle for CO2 insufflation until the pressure established for each group was obtained (4 mmHg or 10 mmHg). Pressure was maintained during one hour, followed by one hour of disinsufflation. At the end of this phase, all animals, still under anesthesia, were submitted to euthanasia by blood removal through the arterial catheter. The blood samples were collected in three phases: 1) post-catheterization; 2) after one hour of pneumoperitoneum, and 3) one hour after disinsufflation. The samples were sent to the laboratory for urea and creatinine measurements. Results: Increased creatinine in SHR rats after 1 h of pneumoperitoneum with 10 mmHg pressure, while Wistar rats remained at the normal basal level. Urea levels increased for both SHR and EPM-1 Wistar rats after 1 h of pneumoperitoneum with 10 mmHg pressure. During the pneumoperitoneum with 4 mmHg pressure, creatinine and urea remained at basal levels. Conclusion: The renal function of SHR rats may be transiently changed, as it happens with non-hypertensive rats submitted to a 10 mmHg pneumoperitoneum.