Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy

Detalhes bibliográficos
Autor(a) principal: Ferreira, Elenn Soares [UNIFESP]
Data de Publicação: 2018
Outros Autores: Vieira, Lais Gabrielle [UNIFESP], Moraes, Daniela Macedo [UNIFESP], Amorim, Beatriz O. [UNIFESP], Malheiros, Jackeline Moraes [UNIFESP], Hamani, Clement, Covolan, Luciene [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1111/ner.12688
https://repositorio.unifesp.br/handle/11600/54214
Resumo: Introduction and ObjectivesCognitive impairment is a significant comorbidity of temporal lobe epilepsy that is associated with extensive hippocampal cell loss. Deep brain stimulation (DBS) of the anterior thalamic nucleus (ANT) has been used for the treatment of refractory partial seizures. In the pilocarpine model of epilepsy, ANT DBS applied during status epilepticus (SE) reduces hippocampal inflammation and apoptosis. When given to chronic epileptic animals it reduces hippocampal excitability and seizure frequency. Here, we tested whether ANT DBS delivered during SE and the silent phase of the pilocarpine model would reduce cognitive impairment when animals became chronically epileptic. Materials and MethodsSE was induced by a systemic pilocarpine injection (320 mg/kg). Immediately after SE onset, rats were assigned to receive DBS during the first six hours of SE (n=8
id UFSP_d4261ddfb70df913869ec884bab3df20
oai_identifier_str oai:repositorio.unifesp.br/:11600/54214
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsydeep brain stimulationhippocampuslearningneuroprotectionpilocarpinethalamuswater mazeIntroduction and ObjectivesCognitive impairment is a significant comorbidity of temporal lobe epilepsy that is associated with extensive hippocampal cell loss. Deep brain stimulation (DBS) of the anterior thalamic nucleus (ANT) has been used for the treatment of refractory partial seizures. In the pilocarpine model of epilepsy, ANT DBS applied during status epilepticus (SE) reduces hippocampal inflammation and apoptosis. When given to chronic epileptic animals it reduces hippocampal excitability and seizure frequency. Here, we tested whether ANT DBS delivered during SE and the silent phase of the pilocarpine model would reduce cognitive impairment when animals became chronically epileptic. Materials and MethodsSE was induced by a systemic pilocarpine injection (320 mg/kg). Immediately after SE onset, rats were assigned to receive DBS during the first six hours of SE (n=8DBSa group) or during SE+the silent period (i.e., 6 h/day until the animals developed the first spontaneous recurrent seizuren=10DBSs group). Four months following SE, animals underwent water maze testing and histological evaluation. Nonstimulated chronic epileptic animals (n=13PCTL group) and age-matched naive rats (n=11, CTL group) were used as controls. Results were analyzed by repeated-measures analyses of variance (RM_ANOVA) and one-way ANOVAs, followed by Newman-Keuls post hoc tests. ResultsAlthough all groups learned the spatial task, epileptic animals with or without DBS spent significantly less time in the platform quadrant, denoting a spatial memory deficit (p<0.02). Despite these negative behavioral results, we found that animals given DBS had a significantly higher number of cells in the CA1 region and dentate gyrus. Mossy fiber sprouting was similar among all epileptic groups. ConclusionsDespite lesser hippocampal neuronal loss, ANT DBS delivered either during SE or during SE and the silent phase of the pilocarpine model did not mitigate memory deficits in chronic epileptic rats.Univ Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862, BR-04023062 Sao Paulo, SP, BrazilCtr Addict & Mental Hlth, Behav Neurobiol Lab, Toronto, ON, CanadaUniv Toronto, Toronto Western Hosp, Div Neurosurg, Toronto, ON, CanadaUniv Fed Sao Paulo, Dept Fisiol, Rua Botucatu 862, BR-04023062 Sao Paulo, SP, BrazilWeb of ScienceFAPESPCNPqCAPESFAPESP [2012/50950-2]CNPq [304021/2015-6]CAPESWiley2020-07-08T13:09:48Z2020-07-08T13:09:48Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion160-167http://dx.doi.org/10.1111/ner.12688Neuromodulation. Hoboken, v. 21, n. 2, p. 160-167, 2018.10.1111/ner.126881094-7159https://repositorio.unifesp.br/handle/11600/54214WOS:000424282700006engNeuromodulationHobokeninfo:eu-repo/semantics/openAccessFerreira, Elenn Soares [UNIFESP]Vieira, Lais Gabrielle [UNIFESP]Moraes, Daniela Macedo [UNIFESP]Amorim, Beatriz O. [UNIFESP]Malheiros, Jackeline Moraes [UNIFESP]Hamani, ClementCovolan, Luciene [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-30T17:31:41Zoai:repositorio.unifesp.br/:11600/54214Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-30T17:31:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
title Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
spellingShingle Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
Ferreira, Elenn Soares [UNIFESP]
deep brain stimulation
hippocampus
learning
neuroprotection
pilocarpine
thalamus
water maze
title_short Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
title_full Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
title_fullStr Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
title_full_unstemmed Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
title_sort Long-Term Effects of Anterior Thalamic Nucleus Deep Brain Stimulation on Spatial Learning in the Pilocarpine Model of Temporal Lobe Epilepsy
author Ferreira, Elenn Soares [UNIFESP]
author_facet Ferreira, Elenn Soares [UNIFESP]
Vieira, Lais Gabrielle [UNIFESP]
Moraes, Daniela Macedo [UNIFESP]
Amorim, Beatriz O. [UNIFESP]
Malheiros, Jackeline Moraes [UNIFESP]
Hamani, Clement
Covolan, Luciene [UNIFESP]
author_role author
author2 Vieira, Lais Gabrielle [UNIFESP]
Moraes, Daniela Macedo [UNIFESP]
Amorim, Beatriz O. [UNIFESP]
Malheiros, Jackeline Moraes [UNIFESP]
Hamani, Clement
Covolan, Luciene [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Elenn Soares [UNIFESP]
Vieira, Lais Gabrielle [UNIFESP]
Moraes, Daniela Macedo [UNIFESP]
Amorim, Beatriz O. [UNIFESP]
Malheiros, Jackeline Moraes [UNIFESP]
Hamani, Clement
Covolan, Luciene [UNIFESP]
dc.subject.por.fl_str_mv deep brain stimulation
hippocampus
learning
neuroprotection
pilocarpine
thalamus
water maze
topic deep brain stimulation
hippocampus
learning
neuroprotection
pilocarpine
thalamus
water maze
description Introduction and ObjectivesCognitive impairment is a significant comorbidity of temporal lobe epilepsy that is associated with extensive hippocampal cell loss. Deep brain stimulation (DBS) of the anterior thalamic nucleus (ANT) has been used for the treatment of refractory partial seizures. In the pilocarpine model of epilepsy, ANT DBS applied during status epilepticus (SE) reduces hippocampal inflammation and apoptosis. When given to chronic epileptic animals it reduces hippocampal excitability and seizure frequency. Here, we tested whether ANT DBS delivered during SE and the silent phase of the pilocarpine model would reduce cognitive impairment when animals became chronically epileptic. Materials and MethodsSE was induced by a systemic pilocarpine injection (320 mg/kg). Immediately after SE onset, rats were assigned to receive DBS during the first six hours of SE (n=8
publishDate 2018
dc.date.none.fl_str_mv 2018
2020-07-08T13:09:48Z
2020-07-08T13:09:48Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/ner.12688
Neuromodulation. Hoboken, v. 21, n. 2, p. 160-167, 2018.
10.1111/ner.12688
1094-7159
https://repositorio.unifesp.br/handle/11600/54214
WOS:000424282700006
url http://dx.doi.org/10.1111/ner.12688
https://repositorio.unifesp.br/handle/11600/54214
identifier_str_mv Neuromodulation. Hoboken, v. 21, n. 2, p. 160-167, 2018.
10.1111/ner.12688
1094-7159
WOS:000424282700006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neuromodulation
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 160-167
dc.coverage.none.fl_str_mv Hoboken
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268302092926976

Registros relacionados