Matriz Metaloproteinase 2: um importante marcador genético para colesteatomas

• Autor(a) principal: Morales, Douglas Salmazo Rocha
• Data de Publicação: 2007
• Outros Autores: Penido, Norma de Oliveira [UNIFESP], Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP], Stávale, João Norberto [UNIFESP], Guilherme, Arnaldo [UNIFESP], Fukuda, Yotaka [UNIFESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1590/S0034-72992007000100009; http://repositorio.unifesp.br/handle/11600/3531
• topic: cholesteatoma; metalloproteinase; colesteatoma; metaloproteinase

AIM: This study is to determine the MMP2 s presence in cholesteatomas and whether complicating cholesteatomas show a higher immunohistochemical expression of matrix metalloproteinase 2. Cholesteatoma produces enzymesthat cause bone erosion like Matrixmetalloproteinase 2 (MMP2). MATERIAL AND METHODS: We analyzed the expression of MMP2 in invasive (causing complications) compared to latent cholesteatomas (not causing complications). A crosssectional study with nineteen slides and paraffin blocks of cholesteatomas derived from mastoidectomies were located and processed, including 8 invasive and 11 latent cholesteatomas. Immunohistochemical thecnique was empregated to MMP2. RESULTS: The results are expressed as 0, + (to low), ++ and +++(high) according to the quantity and color of the immunohistochemical staining of MMP2. Higher expression of MMP2 was observed in 7 (87.5%) of the 8 invasive cholesteatomas. With respect to latent cholesteatomas, higher expression of MMP2 was observed in 27.3% (3 cases), with Fisher s exact test indicating a significant difference (p=0.015). CONCLUSIONS: Cholesteatoamas express MMP2 and Invasive cholesteatomas had high MMP2 compared to latent cholesteatomas.