HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/29456 http://dx.doi.org/10.1002/cyto.b.20152 |
Resumo: | Background: HIV-1-infected children show changes of blood lymphocyte subpopulations. We have, therefore, investigated how highly active anti-retroviral therapy (ART) alter these subsets. Blood samples were taken from 41 HIV-1-infected children on ART who were divided into groups showing good, partial and poor responses to ART on the basis of viral load (VL) measurement in blood. the observations were compared to those seen in 20 uninfected children.Methods: the samples were studied using 4-color flow cytometry for naive, central memory and effector memory cells as well as for CD38 expression as the sign of activation within both the CD4+ and the CD8+ T cell populations. HIV-1 infected children were also evaluated for the presence and the titers of antibodies induced by vaccination against childhood infections in our patients while on HAART.Results: Lymphocyte counts were lower in the poor viral load responding (VLR) group when compared with partial and good VLRs. Poor VLRs had lower total and naive CD4+ T cell counts. HIV-1-infected children from all three groups had high CD8+ T cell counts. Central memory CD4+ and CD8+ T cell percentages were particularly low in the poor VLR group while in the poor VLR group the percentages of effector memory CD4+ and CD8+ T cells were higher when compared with the control group. Higher cellular activation of CD8+ T cells was observed in HIV-1-infected children, particularly when analyzed for the intensity of CD38 expression in the poor VLR group. CD5 expression on B cells was higher among all HIV-1-infected children. Antibodies to tetanus, diphtheria, measles, rubella, and hepatitis B were present in a large proportion of children but the titers were similarly low for all three groups of HIV-infected children.Conclusions: Children with different levels of viral response to HAART present immune phenotype characteristics that tend to place the children with partial and good virological responses into the same group. These children are still moderately deficient in their immune responses but show better recovery than seen with children in the poor VLR group. These observations indicate that the proportions of central memory cells among the CD4+ T cells and the intensity of the expression of CD38 activation antigen on CD8+ T cells provide more informative parameters for monitoring children on HAART than the absolute numbers of CD4+ and CD8+ T cells alone. (c) 2006 Clinical Cytometry Society. |
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Zaccarelli Filho, Carlos Alberto [UNIFESP]Ono, Erika [UNIFESP]Machado, Daisy Maria [UNIFESP]Brunialti, Milena Karina Coló [UNIFESP]Succi, Regina Célia de Menezes [UNIFESP]Salomão, Reinaldo [UNIFESP]Kallas, Esper Georges [UNIFESP]De Moraes-Pinto, Maria Isabel [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2016-01-24T12:41:49Z2016-01-24T12:41:49Z2007-01-15Cytometry Part B-clinical Cytometry. Hoboken: Wiley-liss, v. 72B, n. 1, p. 14-21, 2007.1552-4949http://repositorio.unifesp.br/handle/11600/29456http://dx.doi.org/10.1002/cyto.b.2015210.1002/cyto.b.20152WOS:000243322000004Background: HIV-1-infected children show changes of blood lymphocyte subpopulations. We have, therefore, investigated how highly active anti-retroviral therapy (ART) alter these subsets. Blood samples were taken from 41 HIV-1-infected children on ART who were divided into groups showing good, partial and poor responses to ART on the basis of viral load (VL) measurement in blood. the observations were compared to those seen in 20 uninfected children.Methods: the samples were studied using 4-color flow cytometry for naive, central memory and effector memory cells as well as for CD38 expression as the sign of activation within both the CD4+ and the CD8+ T cell populations. HIV-1 infected children were also evaluated for the presence and the titers of antibodies induced by vaccination against childhood infections in our patients while on HAART.Results: Lymphocyte counts were lower in the poor viral load responding (VLR) group when compared with partial and good VLRs. Poor VLRs had lower total and naive CD4+ T cell counts. HIV-1-infected children from all three groups had high CD8+ T cell counts. Central memory CD4+ and CD8+ T cell percentages were particularly low in the poor VLR group while in the poor VLR group the percentages of effector memory CD4+ and CD8+ T cells were higher when compared with the control group. Higher cellular activation of CD8+ T cells was observed in HIV-1-infected children, particularly when analyzed for the intensity of CD38 expression in the poor VLR group. CD5 expression on B cells was higher among all HIV-1-infected children. Antibodies to tetanus, diphtheria, measles, rubella, and hepatitis B were present in a large proportion of children but the titers were similarly low for all three groups of HIV-infected children.Conclusions: Children with different levels of viral response to HAART present immune phenotype characteristics that tend to place the children with partial and good virological responses into the same group. These children are still moderately deficient in their immune responses but show better recovery than seen with children in the poor VLR group. These observations indicate that the proportions of central memory cells among the CD4+ T cells and the intensity of the expression of CD38 activation antigen on CD8+ T cells provide more informative parameters for monitoring children on HAART than the absolute numbers of CD4+ and CD8+ T cells alone. (c) 2006 Clinical Cytometry Society.Universidade Federal de São Paulo, Res Lab, Div Pediat Infect Dis, BR-04039032 São Paulo, SP, BrazilUniversidade Federal de São Paulo, Div Infect Dis, São Paulo, BrazilUniversidade Federal de São Paulo, Res Lab, Div Pediat Infect Dis, BR-04039032 São Paulo, SP, BrazilUniversidade Federal de São Paulo, Div Infect Dis, São Paulo, BrazilWeb of Science14-21engWiley-BlackwellCytometry Part B-clinical Cytometryhttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccessHIV-infected childrenantiretroviral therapyimmune reconstitutionHIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppressioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/294562022-09-27 09:48:38.485metadata only accessoai:repositorio.unifesp.br:11600/29456Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:23:46.350315Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
title |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
spellingShingle |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression Zaccarelli Filho, Carlos Alberto [UNIFESP] HIV-infected children antiretroviral therapy immune reconstitution |
title_short |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
title_full |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
title_fullStr |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
title_full_unstemmed |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
title_sort |
HIV-1-Infected children on HAART: Immunologic features of three different levels of viral suppression |
author |
Zaccarelli Filho, Carlos Alberto [UNIFESP] |
author_facet |
Zaccarelli Filho, Carlos Alberto [UNIFESP] Ono, Erika [UNIFESP] Machado, Daisy Maria [UNIFESP] Brunialti, Milena Karina Coló [UNIFESP] Succi, Regina Célia de Menezes [UNIFESP] Salomão, Reinaldo [UNIFESP] Kallas, Esper Georges [UNIFESP] De Moraes-Pinto, Maria Isabel [UNIFESP] |
author_role |
author |
author2 |
Ono, Erika [UNIFESP] Machado, Daisy Maria [UNIFESP] Brunialti, Milena Karina Coló [UNIFESP] Succi, Regina Célia de Menezes [UNIFESP] Salomão, Reinaldo [UNIFESP] Kallas, Esper Georges [UNIFESP] De Moraes-Pinto, Maria Isabel [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Zaccarelli Filho, Carlos Alberto [UNIFESP] Ono, Erika [UNIFESP] Machado, Daisy Maria [UNIFESP] Brunialti, Milena Karina Coló [UNIFESP] Succi, Regina Célia de Menezes [UNIFESP] Salomão, Reinaldo [UNIFESP] Kallas, Esper Georges [UNIFESP] De Moraes-Pinto, Maria Isabel [UNIFESP] |
dc.subject.eng.fl_str_mv |
HIV-infected children antiretroviral therapy immune reconstitution |
topic |
HIV-infected children antiretroviral therapy immune reconstitution |
description |
Background: HIV-1-infected children show changes of blood lymphocyte subpopulations. We have, therefore, investigated how highly active anti-retroviral therapy (ART) alter these subsets. Blood samples were taken from 41 HIV-1-infected children on ART who were divided into groups showing good, partial and poor responses to ART on the basis of viral load (VL) measurement in blood. the observations were compared to those seen in 20 uninfected children.Methods: the samples were studied using 4-color flow cytometry for naive, central memory and effector memory cells as well as for CD38 expression as the sign of activation within both the CD4+ and the CD8+ T cell populations. HIV-1 infected children were also evaluated for the presence and the titers of antibodies induced by vaccination against childhood infections in our patients while on HAART.Results: Lymphocyte counts were lower in the poor viral load responding (VLR) group when compared with partial and good VLRs. Poor VLRs had lower total and naive CD4+ T cell counts. HIV-1-infected children from all three groups had high CD8+ T cell counts. Central memory CD4+ and CD8+ T cell percentages were particularly low in the poor VLR group while in the poor VLR group the percentages of effector memory CD4+ and CD8+ T cells were higher when compared with the control group. Higher cellular activation of CD8+ T cells was observed in HIV-1-infected children, particularly when analyzed for the intensity of CD38 expression in the poor VLR group. CD5 expression on B cells was higher among all HIV-1-infected children. Antibodies to tetanus, diphtheria, measles, rubella, and hepatitis B were present in a large proportion of children but the titers were similarly low for all three groups of HIV-infected children.Conclusions: Children with different levels of viral response to HAART present immune phenotype characteristics that tend to place the children with partial and good virological responses into the same group. These children are still moderately deficient in their immune responses but show better recovery than seen with children in the poor VLR group. These observations indicate that the proportions of central memory cells among the CD4+ T cells and the intensity of the expression of CD38 activation antigen on CD8+ T cells provide more informative parameters for monitoring children on HAART than the absolute numbers of CD4+ and CD8+ T cells alone. (c) 2006 Clinical Cytometry Society. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-01-15 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:41:49Z |
dc.date.available.fl_str_mv |
2016-01-24T12:41:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Cytometry Part B-clinical Cytometry. Hoboken: Wiley-liss, v. 72B, n. 1, p. 14-21, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/29456 http://dx.doi.org/10.1002/cyto.b.20152 |
dc.identifier.issn.none.fl_str_mv |
1552-4949 |
dc.identifier.doi.none.fl_str_mv |
10.1002/cyto.b.20152 |
dc.identifier.wos.none.fl_str_mv |
WOS:000243322000004 |
identifier_str_mv |
Cytometry Part B-clinical Cytometry. Hoboken: Wiley-liss, v. 72B, n. 1, p. 14-21, 2007. 1552-4949 10.1002/cyto.b.20152 WOS:000243322000004 |
url |
http://repositorio.unifesp.br/handle/11600/29456 http://dx.doi.org/10.1002/cyto.b.20152 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Cytometry Part B-clinical Cytometry |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14-21 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460285457104896 |