Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes

Detalhes bibliográficos
Autor(a) principal: Goldman, Gustavo H.
Data de Publicação: 2003
Outros Autores: Marques, Everaldo dos Reis, Ribeiro, Diogenes Custodio Duarte, Bernardes, Luciano Angelo de Souza, Quiapin, Andrea Carla, Vitorelli, Patricia Marostica, Savoldi, Marcela, Semighini, Camile P., Oliveira, Regina C. de, Nunes, Luiz R., Travassos, Luiz R. [UNIFESP], Puccia, Rosana [UNIFESP], Batista, Wagner L. [UNIFESP], Ferreira, Leslie Ecker., Moreira, Julio C., Bogossian, Ana Paula, Tekaia, Fredj, Nobrega, Marina Pasetto, Nobrega, Francisco G., Goldman, Maria Helena S
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1128/EC.2.1.34-48.2003
http://repositorio.unifesp.br/handle/11600/27124
Resumo: Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities.
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spelling Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genesParacoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities.Univ São Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, BrazilUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-14040903 Ribeirao Preto, SP, BrazilInst Pasteur, Unite Genet Mol Levures, Paris, FranceUniv Vale do Paraiba, UNIVAP, Vale Do Paraiba, BrazilUniv Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceAmer Soc MicrobiologyUniversidade de São Paulo (USP)Inst PasteurUniv Vale do ParaibaUniv Mogi das CruzesUniversidade Federal de São Paulo (UNIFESP)Goldman, Gustavo H.Marques, Everaldo dos ReisRibeiro, Diogenes Custodio DuarteBernardes, Luciano Angelo de SouzaQuiapin, Andrea CarlaVitorelli, Patricia MarosticaSavoldi, MarcelaSemighini, Camile P.Oliveira, Regina C. deNunes, Luiz R.Travassos, Luiz R. [UNIFESP]Puccia, Rosana [UNIFESP]Batista, Wagner L. [UNIFESP]Ferreira, Leslie Ecker.Moreira, Julio C.Bogossian, Ana PaulaTekaia, FredjNobrega, Marina PasettoNobrega, Francisco G.Goldman, Maria Helena S2016-01-24T12:33:42Z2016-01-24T12:33:42Z2003-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion34-48application/pdfhttp://dx.doi.org/10.1128/EC.2.1.34-48.2003Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003.10.1128/EC.2.1.34-48.2003WOS000180988400005.pdf1535-9778http://repositorio.unifesp.br/handle/11600/27124WOS:000180988400005engEukaryotic Cellinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T10:38:08Zoai:repositorio.unifesp.br/:11600/27124Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T10:38:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
title Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
spellingShingle Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
Goldman, Gustavo H.
title_short Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
title_full Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
title_fullStr Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
title_full_unstemmed Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
title_sort Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
author Goldman, Gustavo H.
author_facet Goldman, Gustavo H.
Marques, Everaldo dos Reis
Ribeiro, Diogenes Custodio Duarte
Bernardes, Luciano Angelo de Souza
Quiapin, Andrea Carla
Vitorelli, Patricia Marostica
Savoldi, Marcela
Semighini, Camile P.
Oliveira, Regina C. de
Nunes, Luiz R.
Travassos, Luiz R. [UNIFESP]
Puccia, Rosana [UNIFESP]
Batista, Wagner L. [UNIFESP]
Ferreira, Leslie Ecker.
Moreira, Julio C.
Bogossian, Ana Paula
Tekaia, Fredj
Nobrega, Marina Pasetto
Nobrega, Francisco G.
Goldman, Maria Helena S
author_role author
author2 Marques, Everaldo dos Reis
Ribeiro, Diogenes Custodio Duarte
Bernardes, Luciano Angelo de Souza
Quiapin, Andrea Carla
Vitorelli, Patricia Marostica
Savoldi, Marcela
Semighini, Camile P.
Oliveira, Regina C. de
Nunes, Luiz R.
Travassos, Luiz R. [UNIFESP]
Puccia, Rosana [UNIFESP]
Batista, Wagner L. [UNIFESP]
Ferreira, Leslie Ecker.
Moreira, Julio C.
Bogossian, Ana Paula
Tekaia, Fredj
Nobrega, Marina Pasetto
Nobrega, Francisco G.
Goldman, Maria Helena S
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Inst Pasteur
Univ Vale do Paraiba
Univ Mogi das Cruzes
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Goldman, Gustavo H.
Marques, Everaldo dos Reis
Ribeiro, Diogenes Custodio Duarte
Bernardes, Luciano Angelo de Souza
Quiapin, Andrea Carla
Vitorelli, Patricia Marostica
Savoldi, Marcela
Semighini, Camile P.
Oliveira, Regina C. de
Nunes, Luiz R.
Travassos, Luiz R. [UNIFESP]
Puccia, Rosana [UNIFESP]
Batista, Wagner L. [UNIFESP]
Ferreira, Leslie Ecker.
Moreira, Julio C.
Bogossian, Ana Paula
Tekaia, Fredj
Nobrega, Marina Pasetto
Nobrega, Francisco G.
Goldman, Maria Helena S
description Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities.
publishDate 2003
dc.date.none.fl_str_mv 2003-02-01
2016-01-24T12:33:42Z
2016-01-24T12:33:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1128/EC.2.1.34-48.2003
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003.
10.1128/EC.2.1.34-48.2003
WOS000180988400005.pdf
1535-9778
http://repositorio.unifesp.br/handle/11600/27124
WOS:000180988400005
url http://dx.doi.org/10.1128/EC.2.1.34-48.2003
http://repositorio.unifesp.br/handle/11600/27124
identifier_str_mv Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003.
10.1128/EC.2.1.34-48.2003
WOS000180988400005.pdf
1535-9778
WOS:000180988400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eukaryotic Cell
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 34-48
application/pdf
dc.publisher.none.fl_str_mv Amer Soc Microbiology
publisher.none.fl_str_mv Amer Soc Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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