Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1128/EC.2.1.34-48.2003 http://repositorio.unifesp.br/handle/11600/27124 |
Resumo: | Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities. |
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Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genesParacoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities.Univ São Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP, BrazilUniv São Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-14040903 Ribeirao Preto, SP, BrazilInst Pasteur, Unite Genet Mol Levures, Paris, FranceUniv Vale do Paraiba, UNIVAP, Vale Do Paraiba, BrazilUniv Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceAmer Soc MicrobiologyUniversidade de São Paulo (USP)Inst PasteurUniv Vale do ParaibaUniv Mogi das CruzesUniversidade Federal de São Paulo (UNIFESP)Goldman, Gustavo H.Marques, Everaldo dos ReisRibeiro, Diogenes Custodio DuarteBernardes, Luciano Angelo de SouzaQuiapin, Andrea CarlaVitorelli, Patricia MarosticaSavoldi, MarcelaSemighini, Camile P.Oliveira, Regina C. deNunes, Luiz R.Travassos, Luiz R. [UNIFESP]Puccia, Rosana [UNIFESP]Batista, Wagner L. [UNIFESP]Ferreira, Leslie Ecker.Moreira, Julio C.Bogossian, Ana PaulaTekaia, FredjNobrega, Marina PasettoNobrega, Francisco G.Goldman, Maria Helena S2016-01-24T12:33:42Z2016-01-24T12:33:42Z2003-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion34-48application/pdfhttp://dx.doi.org/10.1128/EC.2.1.34-48.2003Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003.10.1128/EC.2.1.34-48.2003WOS000180988400005.pdf1535-9778http://repositorio.unifesp.br/handle/11600/27124WOS:000180988400005engEukaryotic Cellinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-07T10:38:08Zoai:repositorio.unifesp.br/:11600/27124Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-07T10:38:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
title |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
spellingShingle |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes Goldman, Gustavo H. |
title_short |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
title_full |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
title_fullStr |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
title_full_unstemmed |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
title_sort |
Expressed sequence tag analysis of the human pathogen Paracoccidioides brasiliensis yeast phase: Identification of putative homologues of Candida albicans virulence and pathogenicity genes |
author |
Goldman, Gustavo H. |
author_facet |
Goldman, Gustavo H. Marques, Everaldo dos Reis Ribeiro, Diogenes Custodio Duarte Bernardes, Luciano Angelo de Souza Quiapin, Andrea Carla Vitorelli, Patricia Marostica Savoldi, Marcela Semighini, Camile P. Oliveira, Regina C. de Nunes, Luiz R. Travassos, Luiz R. [UNIFESP] Puccia, Rosana [UNIFESP] Batista, Wagner L. [UNIFESP] Ferreira, Leslie Ecker. Moreira, Julio C. Bogossian, Ana Paula Tekaia, Fredj Nobrega, Marina Pasetto Nobrega, Francisco G. Goldman, Maria Helena S |
author_role |
author |
author2 |
Marques, Everaldo dos Reis Ribeiro, Diogenes Custodio Duarte Bernardes, Luciano Angelo de Souza Quiapin, Andrea Carla Vitorelli, Patricia Marostica Savoldi, Marcela Semighini, Camile P. Oliveira, Regina C. de Nunes, Luiz R. Travassos, Luiz R. [UNIFESP] Puccia, Rosana [UNIFESP] Batista, Wagner L. [UNIFESP] Ferreira, Leslie Ecker. Moreira, Julio C. Bogossian, Ana Paula Tekaia, Fredj Nobrega, Marina Pasetto Nobrega, Francisco G. Goldman, Maria Helena S |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Inst Pasteur Univ Vale do Paraiba Univ Mogi das Cruzes Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Goldman, Gustavo H. Marques, Everaldo dos Reis Ribeiro, Diogenes Custodio Duarte Bernardes, Luciano Angelo de Souza Quiapin, Andrea Carla Vitorelli, Patricia Marostica Savoldi, Marcela Semighini, Camile P. Oliveira, Regina C. de Nunes, Luiz R. Travassos, Luiz R. [UNIFESP] Puccia, Rosana [UNIFESP] Batista, Wagner L. [UNIFESP] Ferreira, Leslie Ecker. Moreira, Julio C. Bogossian, Ana Paula Tekaia, Fredj Nobrega, Marina Pasetto Nobrega, Francisco G. Goldman, Maria Helena S |
description |
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis. We present here a survey of expressed genes in the yeast pathogenic phase of P. brasiliensis. We obtained 13,490 expressed sequence tags from both 5' and 3' ends. Clustering analysis yielded the partial sequences of 4,692 expressed genes that were functionally classified by similarity to known genes. We have identified several Candida albicans virulence and pathogenicity homologues in P. brasiliensis. Furthermore, we have analyzed the expression of some of these genes during the dimorphic yeast-mycelium-yeast transition by real-time quantitative reverse transcription-PCR. Clustering analysis of the mycelium-yeast transition revealed three groups: (i) RBT, hydrophobin, and isocitrate lyase; (ii) malate dehydrogenase, contigs Pb1067 and Pb1145, GPI, and alternative oxidase; and (iii) ubiquitin, delta-9-desaturase, HSP70, HSP82, and HSP104. the first two groups displayed high mRNA expression in the mycelial phase, whereas the third group showed higher mRNA expression in the yeast phase. Our results suggest the possible conservation of pathogenicity and virulence mechanisms among fungi, expand considerably gene identification in P. brasiliensis, and provide a broader basis for further progress in understanding its biological peculiarities. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-02-01 2016-01-24T12:33:42Z 2016-01-24T12:33:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/EC.2.1.34-48.2003 Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003. 10.1128/EC.2.1.34-48.2003 WOS000180988400005.pdf 1535-9778 http://repositorio.unifesp.br/handle/11600/27124 WOS:000180988400005 |
url |
http://dx.doi.org/10.1128/EC.2.1.34-48.2003 http://repositorio.unifesp.br/handle/11600/27124 |
identifier_str_mv |
Eukaryotic Cell. Washington: Amer Soc Microbiology, v. 2, n. 1, p. 34-48, 2003. 10.1128/EC.2.1.34-48.2003 WOS000180988400005.pdf 1535-9778 WOS:000180988400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Eukaryotic Cell |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
34-48 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268458075947008 |