Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke
Main Author: | |
---|---|
Publication Date: | 2013 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNIFESP |
Download full: | http://repositorio.unifesp.br/handle/11600/7836 http://dx.doi.org/10.6061/clinics/2013(06)21 |
Summary: | OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth cerebral ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats. |
id |
UFSP_d80dd8fc12f5eaf0a8e3547a5b303837 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br:11600/7836 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Valenti, Vitor Engrácia [UNIFESP]Abreu, Luiz Carlos deFonseca, Fernando Luiz Affonso [UNIFESP]Adami, FernandoSato, Monica AkemiVanderlei, Luiz Carlos MarquesFerreira, Lucas LimaRodrigues, Luciano M.Ferreira, Celso [UNIFESP]Universidade Estadual Paulista (UNESP)Faculdade de Medicina do ABC Departamento de Morfologia e FisiologiaUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:45:30Z2015-06-14T13:45:30Z2013-06-01Clinics. Faculdade de Medicina / USP, v. 68, n. 6, p. 851-857, 2013.1807-5932http://repositorio.unifesp.br/handle/11600/7836http://dx.doi.org/10.6061/clinics/2013(06)21S1807-59322013000600851.pdfS1807-5932201300060085110.6061/clinics/2013(06)21WOS:000320463800021OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth cerebral ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats.Foundation of Support to Research of Sao Paulo State (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP)Universidade Estadual Paulista Faculdade de Ciências e TecnologiaFaculdade de Medicina do ABC Departamento de Morfologia e FisiologiaUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPM10/17769-7SciELO851-857engFaculdade de Medicina / USPClinicsOxidative StressCatalaseMedulla OblongataTobaccoAir PollutantsEffects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smokeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1807-59322013000600851.pdfapplication/pdf327222${dspace.ui.url}/bitstream/11600/7836/1/S1807-59322013000600851.pdfa5bfaa75b61eec303daa4c6d975ee9b1MD51open accessTEXTS1807-59322013000600851.pdf.txtS1807-59322013000600851.pdf.txtExtracted texttext/plain40661${dspace.ui.url}/bitstream/11600/7836/2/S1807-59322013000600851.pdf.txtd53259595021817e932092b3f56da4d4MD52open access11600/78362022-07-08 10:58:18.687open accessoai:repositorio.unifesp.br:11600/7836Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:58:18Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
title |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
spellingShingle |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke Valenti, Vitor Engrácia [UNIFESP] Oxidative Stress Catalase Medulla Oblongata Tobacco Air Pollutants |
title_short |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
title_full |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
title_fullStr |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
title_full_unstemmed |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
title_sort |
Effects of the administration of a catalase inhibitor into the fourth cerebral ventricle on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke |
author |
Valenti, Vitor Engrácia [UNIFESP] |
author_facet |
Valenti, Vitor Engrácia [UNIFESP] Abreu, Luiz Carlos de Fonseca, Fernando Luiz Affonso [UNIFESP] Adami, Fernando Sato, Monica Akemi Vanderlei, Luiz Carlos Marques Ferreira, Lucas Lima Rodrigues, Luciano M. Ferreira, Celso [UNIFESP] |
author_role |
author |
author2 |
Abreu, Luiz Carlos de Fonseca, Fernando Luiz Affonso [UNIFESP] Adami, Fernando Sato, Monica Akemi Vanderlei, Luiz Carlos Marques Ferreira, Lucas Lima Rodrigues, Luciano M. Ferreira, Celso [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculdade de Medicina do ABC Departamento de Morfologia e Fisiologia Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Valenti, Vitor Engrácia [UNIFESP] Abreu, Luiz Carlos de Fonseca, Fernando Luiz Affonso [UNIFESP] Adami, Fernando Sato, Monica Akemi Vanderlei, Luiz Carlos Marques Ferreira, Lucas Lima Rodrigues, Luciano M. Ferreira, Celso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Oxidative Stress Catalase Medulla Oblongata Tobacco Air Pollutants |
topic |
Oxidative Stress Catalase Medulla Oblongata Tobacco Air Pollutants |
description |
OBJECTIVE: Previous studies have demonstrated a relationship between brain oxidative stress and cardiovascular regulation. We evaluated the effects of central catalase inhibition on cardiovascular responses in spontaneously hypertensive rats exposed to sidestream cigarette smoke. METHODS: Male Wistar Kyoto (WKY) rats and spontaneously hypertensive rats (SH) (16 weeks old) were implanted with a stainless steel guide cannula leading into the fourth cerebral ventricle (4th V). The femoral artery and vein were cannulated for arterial pressure and heart rate measurement and drug infusion, respectively. The rats were exposed to sidestream cigarette smoke for 180 minutes/day, 5 days/week for 3 weeks (CO: 100-300 ppm). The baroreflex was tested using a pressor dose of phenylephrine (8 μg/kg, bolus) and a depressor dose of sodium nitroprusside (50 μg/kg, bolus). Cardiovascular responses were evaluated before and 5, 15, 30 and 60 minutes after injection of a catalase inhibitor (3-amino-1,2,4-triazole, 0.001 g/100 μL) into the 4th V. RESULTS: Vehicle administration into the 4th V did not affect the cardiovascular response, whereas administration of the central catalase inhibitor increased the basal HR and attenuated the bradycardic peak (p<0.05) to a greater extent in WKY rats exposed to sidestream cigarette smoke than in WKY rats exposed to fresh air. However, in spontaneously hypertensive rats, the effect of the catalase inhibitor treatment was stronger in the fresh air condition (p<0.05). CONCLUSION: Administration of a catalase inhibitor into the 4th V combined with exposure to sidestream cigarette smoke has a stronger effect in WKY rats than in SH rats. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-06-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:45:30Z |
dc.date.available.fl_str_mv |
2015-06-14T13:45:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinics. Faculdade de Medicina / USP, v. 68, n. 6, p. 851-857, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/7836 http://dx.doi.org/10.6061/clinics/2013(06)21 |
dc.identifier.issn.none.fl_str_mv |
1807-5932 |
dc.identifier.file.none.fl_str_mv |
S1807-59322013000600851.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1807-59322013000600851 |
dc.identifier.doi.none.fl_str_mv |
10.6061/clinics/2013(06)21 |
dc.identifier.wos.none.fl_str_mv |
WOS:000320463800021 |
identifier_str_mv |
Clinics. Faculdade de Medicina / USP, v. 68, n. 6, p. 851-857, 2013. 1807-5932 S1807-59322013000600851.pdf S1807-59322013000600851 10.6061/clinics/2013(06)21 WOS:000320463800021 |
url |
http://repositorio.unifesp.br/handle/11600/7836 http://dx.doi.org/10.6061/clinics/2013(06)21 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Clinics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
851-857 |
dc.publisher.none.fl_str_mv |
Faculdade de Medicina / USP |
publisher.none.fl_str_mv |
Faculdade de Medicina / USP |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
bitstream.url.fl_str_mv |
${dspace.ui.url}/bitstream/11600/7836/1/S1807-59322013000600851.pdf ${dspace.ui.url}/bitstream/11600/7836/2/S1807-59322013000600851.pdf.txt |
bitstream.checksum.fl_str_mv |
a5bfaa75b61eec303daa4c6d975ee9b1 d53259595021817e932092b3f56da4d4 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764157602234368 |