Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock
Autor(a) principal: | |
---|---|
Data de Publicação: | 1997 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/531 http://dx.doi.org/10.1590/S0100-879X1997001000012 |
Resumo: | An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem to be regulated by the interaction between Ach and its receptor. Thus, a decrease in Achase activity would suggest decreased cholinergic activity. Adult male Wistar rats received one ECS (80 mA, 0.2 s, 60 Hz) daily for 7 days. Control rats were handled in the same way without receiving the shock. Rats were sacrificed 24 h after the last ECS and membrane-bound and soluble Achase activity was assayed in homogenates obtained from the pons and medulla oblongata. A statistically significant decrease in membrane-bound Achase activity (nmol thiocholine formed min-1 mg protein-1) (control 182.6 ± 14.8, ECS 162.2 ± 14.2, P<0.05) and an increase in soluble Achase activity in the medulla oblongata (control 133.6 ± 4.2, ECS 145.8 ± 12.3, P<0.05) were observed. No statistical differences were observed in Achase activity in the pons. Although repeated ECS induced a decrease in membrane-bound Achase activity, the lack of changes in the pons (control Achase activity: total 231.0 ± 34.5, membrane-bound 298.9 ± 18.5, soluble 203.9 ± 30.9), the region where the locus coeruleus, the main noradrenergic nucleus, is located, does not seem to favor the existence of an interaction between cholinergic and noradrenergic neurotransmission after ECS treatment |
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Camarini, Rosana [UNIFESP]Venditti, Marco Antonio Campana [UNIFESP]Universidade Federal de São Paulo (UNIFESP)2015-06-14T13:24:38Z2015-06-14T13:24:38Z1997-10-01Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 30, n. 10, p. 1215-1218, 1997.0100-879Xhttp://repositorio.unifesp.br/handle/11600/531http://dx.doi.org/10.1590/S0100-879X1997001000012S0100-879X1997001000012.pdfS0100-879X199700100001210.1590/S0100-879X1997001000012WOS:A1997YA76600012An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem to be regulated by the interaction between Ach and its receptor. Thus, a decrease in Achase activity would suggest decreased cholinergic activity. Adult male Wistar rats received one ECS (80 mA, 0.2 s, 60 Hz) daily for 7 days. Control rats were handled in the same way without receiving the shock. Rats were sacrificed 24 h after the last ECS and membrane-bound and soluble Achase activity was assayed in homogenates obtained from the pons and medulla oblongata. A statistically significant decrease in membrane-bound Achase activity (nmol thiocholine formed min-1 mg protein-1) (control 182.6 ± 14.8, ECS 162.2 ± 14.2, P<0.05) and an increase in soluble Achase activity in the medulla oblongata (control 133.6 ± 4.2, ECS 145.8 ± 12.3, P<0.05) were observed. No statistical differences were observed in Achase activity in the pons. Although repeated ECS induced a decrease in membrane-bound Achase activity, the lack of changes in the pons (control Achase activity: total 231.0 ± 34.5, membrane-bound 298.9 ± 18.5, soluble 203.9 ± 30.9), the region where the locus coeruleus, the main noradrenergic nucleus, is located, does not seem to favor the existence of an interaction between cholinergic and noradrenergic neurotransmission after ECS treatmentUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPM, São PauloSciELO1215-1218engAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Researchacetylcholinesterasemedulla oblongataponschronic electroconvulsive shockAcetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shockinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X1997001000012.pdfapplication/pdf146958${dspace.ui.url}/bitstream/11600/531/1/S0100-879X1997001000012.pdf4b529d24f75e09c067a8e7bad29a3c17MD51open accessTEXTS0100-879X1997001000012.pdf.txtS0100-879X1997001000012.pdf.txtExtracted texttext/plain14956${dspace.ui.url}/bitstream/11600/531/2/S0100-879X1997001000012.pdf.txtb0e1de4bf98cfaecae50a3f6ae8ce405MD52open access11600/5312022-09-27 11:31:14.914open accessoai:repositorio.unifesp.br:11600/531Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:25:50.004393Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
title |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
spellingShingle |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock Camarini, Rosana [UNIFESP] acetylcholinesterase medulla oblongata pons chronic electroconvulsive shock |
title_short |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
title_full |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
title_fullStr |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
title_full_unstemmed |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
title_sort |
Acetylcholinesterase activity in the pons and medulla oblongata of rats after chronic electroconvulsive shock |
author |
Camarini, Rosana [UNIFESP] |
author_facet |
Camarini, Rosana [UNIFESP] Venditti, Marco Antonio Campana [UNIFESP] |
author_role |
author |
author2 |
Venditti, Marco Antonio Campana [UNIFESP] |
author2_role |
author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Camarini, Rosana [UNIFESP] Venditti, Marco Antonio Campana [UNIFESP] |
dc.subject.eng.fl_str_mv |
acetylcholinesterase medulla oblongata pons chronic electroconvulsive shock |
topic |
acetylcholinesterase medulla oblongata pons chronic electroconvulsive shock |
description |
An imbalance between cholinergic and noradrenergic neurotransmission has been proposed for the etiology of affective disorders. According to this hypothesis, depression would be the result of enhanced cholinergic and reduced noradrenergic neurotransmission. Repeated electroconvulsive shock (ECS) is an effective treatment for depression; moreover, in laboratory animals it induces changes in brain noradrenergic neurotransmission similar to those obtained by chronic treatment with antidepressant drugs (down-regulation of beta-adrenergic receptors). The aim of the present study was to determine whether repeated ECS in rats changes acetylcholinesterase (Achase) activity. Achase controls the level of acetylcholine (Ach) in the synaptic cleft and its levels seem to be regulated by the interaction between Ach and its receptor. Thus, a decrease in Achase activity would suggest decreased cholinergic activity. Adult male Wistar rats received one ECS (80 mA, 0.2 s, 60 Hz) daily for 7 days. Control rats were handled in the same way without receiving the shock. Rats were sacrificed 24 h after the last ECS and membrane-bound and soluble Achase activity was assayed in homogenates obtained from the pons and medulla oblongata. A statistically significant decrease in membrane-bound Achase activity (nmol thiocholine formed min-1 mg protein-1) (control 182.6 ± 14.8, ECS 162.2 ± 14.2, P<0.05) and an increase in soluble Achase activity in the medulla oblongata (control 133.6 ± 4.2, ECS 145.8 ± 12.3, P<0.05) were observed. No statistical differences were observed in Achase activity in the pons. Although repeated ECS induced a decrease in membrane-bound Achase activity, the lack of changes in the pons (control Achase activity: total 231.0 ± 34.5, membrane-bound 298.9 ± 18.5, soluble 203.9 ± 30.9), the region where the locus coeruleus, the main noradrenergic nucleus, is located, does not seem to favor the existence of an interaction between cholinergic and noradrenergic neurotransmission after ECS treatment |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997-10-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:24:38Z |
dc.date.available.fl_str_mv |
2015-06-14T13:24:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 30, n. 10, p. 1215-1218, 1997. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/531 http://dx.doi.org/10.1590/S0100-879X1997001000012 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
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S0100-879X1997001000012.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X1997001000012 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-879X1997001000012 |
dc.identifier.wos.none.fl_str_mv |
WOS:A1997YA76600012 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 30, n. 10, p. 1215-1218, 1997. 0100-879X S0100-879X1997001000012.pdf S0100-879X1997001000012 10.1590/S0100-879X1997001000012 WOS:A1997YA76600012 |
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http://repositorio.unifesp.br/handle/11600/531 http://dx.doi.org/10.1590/S0100-879X1997001000012 |
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Brazilian Journal of Medical and Biological Research |
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Associação Brasileira de Divulgação Científica |
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Associação Brasileira de Divulgação Científica |
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