Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/35893 http://dx.doi.org/10.1016/j.ygcen.2012.12.002 |
Resumo: | Bullfrog stem spermatogonia, also named primordial germ cells (PGCs), show strong testosterone immunolabeling in winter, but no or weak testosterone immunoexpression in summer. Thus, the role of testosterone in these cells needs to be clarified. in this study, we proposed to evaluate whether PGCs express aromatase and estrogen receptors, and verify a possible role of estrogen in PGCs seasonal proliferation. Testes of male adult bullfrogs, collected in winter (WG) and summer (SG), were fixed and embedded in historesin, for quantitative analysis, or paraffin for immunohistochemistry (IHC). the number of haematoxylin/eosin stained PGCs/lobular area was obtained. Proliferating cell nuclear antigen (PCNA), aromatase, estrogen receptor beta (ER beta) and PCNA/ER beta double immunolabeling were detected by IHC. the number of PCNA-positive PGCs and the histological score (HSCORE) of aromatase and ER beta immunolabeled PGCs were obtained. Although the number of PGCs increased significantly in WG, a high number of PCNA-positive PGCs was observed in summer. Moreover, aromatase and ER beta HSCORE was higher in SG than WG. the results indicate that PGCs express a seasonal proliferative activity; the low mitotic activity in winter is related to the maximal limit of germ cells which can be supported in the large lobules. in SG, the increased ER beta and aromatase HSCORE suggests that testosterone is converted into estrogen from winter to summer. Moreover, the parallelism between the high PGCs mitotic activity and ER beta immunoexpression suggest a participation of estrogen in the control of the PGCs seasonal proliferative activity which guarantee the formation of new germ cysts from summer to next autumn. (c) 2012 Elsevier Inc. All rights reserved. |
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Caneguim, Breno Henrique [UNIFESP]Luz, Juliana Silva daValentini, Sandro RobertoCerri, Paulo Sergio [UNIFESP]Cerri, Estela Sasso [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Univ Estadual Paulista2016-01-24T14:31:09Z2016-01-24T14:31:09Z2013-02-01General and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 182, p. 65-72, 2013.0016-6480http://repositorio.unifesp.br/handle/11600/35893http://dx.doi.org/10.1016/j.ygcen.2012.12.002WOS000315013100008.pdf10.1016/j.ygcen.2012.12.002WOS:000315013100008Bullfrog stem spermatogonia, also named primordial germ cells (PGCs), show strong testosterone immunolabeling in winter, but no or weak testosterone immunoexpression in summer. Thus, the role of testosterone in these cells needs to be clarified. in this study, we proposed to evaluate whether PGCs express aromatase and estrogen receptors, and verify a possible role of estrogen in PGCs seasonal proliferation. Testes of male adult bullfrogs, collected in winter (WG) and summer (SG), were fixed and embedded in historesin, for quantitative analysis, or paraffin for immunohistochemistry (IHC). the number of haematoxylin/eosin stained PGCs/lobular area was obtained. Proliferating cell nuclear antigen (PCNA), aromatase, estrogen receptor beta (ER beta) and PCNA/ER beta double immunolabeling were detected by IHC. the number of PCNA-positive PGCs and the histological score (HSCORE) of aromatase and ER beta immunolabeled PGCs were obtained. Although the number of PGCs increased significantly in WG, a high number of PCNA-positive PGCs was observed in summer. Moreover, aromatase and ER beta HSCORE was higher in SG than WG. the results indicate that PGCs express a seasonal proliferative activity; the low mitotic activity in winter is related to the maximal limit of germ cells which can be supported in the large lobules. in SG, the increased ER beta and aromatase HSCORE suggests that testosterone is converted into estrogen from winter to summer. Moreover, the parallelism between the high PGCs mitotic activity and ER beta immunoexpression suggest a participation of estrogen in the control of the PGCs seasonal proliferative activity which guarantee the formation of new germ cysts from summer to next autumn. (c) 2012 Elsevier Inc. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Fed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilUniv Estadual Paulista, UNESP, Sch Pharmaceut Sci, Dept Biol Sci, Araraquara, BrazilUniv Estadual Paulista, UNESP, Sch Dent, Dept Morphol,Lab Histol & Embryol, Araraquara, BrazilFed Univ São Paulo UNIFESP, Dept Morphol & Genet, São Paulo, BrazilFAPESP: 2009/17895-5FUNDUNESP: 00661/04-DFPWeb of Science65-72engElsevier B.V.General and Comparative Endocrinologyhttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyinfo:eu-repo/semantics/openAccessAnuraTestesTestosteroneEstrogenImmunohistochemistryProliferating cell nuclear antigenImmunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000315013100008.pdfapplication/pdf2016297${dspace.ui.url}/bitstream/11600/35893/1/WOS000315013100008.pdf4a20fbc07bffa527a759b6538238df57MD51open accessTEXTWOS000315013100008.pdf.txtWOS000315013100008.pdf.txtExtracted texttext/plain42888${dspace.ui.url}/bitstream/11600/35893/2/WOS000315013100008.pdf.txta655a5fff439a7a2296b28f1fbe4e5e8MD52open access11600/358932022-07-08 10:58:16.351open accessoai:repositorio.unifesp.br:11600/35893Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-07-08T13:58:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
title |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
spellingShingle |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity Caneguim, Breno Henrique [UNIFESP] Anura Testes Testosterone Estrogen Immunohistochemistry Proliferating cell nuclear antigen |
title_short |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
title_full |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
title_fullStr |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
title_full_unstemmed |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
title_sort |
Immunoexpression of aromatase and estrogen receptors beta in stem spermatogonia of bullfrogs indicates a role of estrogen in the seasonal spermatogonial mitotic activity |
author |
Caneguim, Breno Henrique [UNIFESP] |
author_facet |
Caneguim, Breno Henrique [UNIFESP] Luz, Juliana Silva da Valentini, Sandro Roberto Cerri, Paulo Sergio [UNIFESP] Cerri, Estela Sasso [UNIFESP] |
author_role |
author |
author2 |
Luz, Juliana Silva da Valentini, Sandro Roberto Cerri, Paulo Sergio [UNIFESP] Cerri, Estela Sasso [UNIFESP] |
author2_role |
author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Estadual Paulista |
dc.contributor.author.fl_str_mv |
Caneguim, Breno Henrique [UNIFESP] Luz, Juliana Silva da Valentini, Sandro Roberto Cerri, Paulo Sergio [UNIFESP] Cerri, Estela Sasso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Anura Testes Testosterone Estrogen Immunohistochemistry Proliferating cell nuclear antigen |
topic |
Anura Testes Testosterone Estrogen Immunohistochemistry Proliferating cell nuclear antigen |
description |
Bullfrog stem spermatogonia, also named primordial germ cells (PGCs), show strong testosterone immunolabeling in winter, but no or weak testosterone immunoexpression in summer. Thus, the role of testosterone in these cells needs to be clarified. in this study, we proposed to evaluate whether PGCs express aromatase and estrogen receptors, and verify a possible role of estrogen in PGCs seasonal proliferation. Testes of male adult bullfrogs, collected in winter (WG) and summer (SG), were fixed and embedded in historesin, for quantitative analysis, or paraffin for immunohistochemistry (IHC). the number of haematoxylin/eosin stained PGCs/lobular area was obtained. Proliferating cell nuclear antigen (PCNA), aromatase, estrogen receptor beta (ER beta) and PCNA/ER beta double immunolabeling were detected by IHC. the number of PCNA-positive PGCs and the histological score (HSCORE) of aromatase and ER beta immunolabeled PGCs were obtained. Although the number of PGCs increased significantly in WG, a high number of PCNA-positive PGCs was observed in summer. Moreover, aromatase and ER beta HSCORE was higher in SG than WG. the results indicate that PGCs express a seasonal proliferative activity; the low mitotic activity in winter is related to the maximal limit of germ cells which can be supported in the large lobules. in SG, the increased ER beta and aromatase HSCORE suggests that testosterone is converted into estrogen from winter to summer. Moreover, the parallelism between the high PGCs mitotic activity and ER beta immunoexpression suggest a participation of estrogen in the control of the PGCs seasonal proliferative activity which guarantee the formation of new germ cysts from summer to next autumn. (c) 2012 Elsevier Inc. All rights reserved. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-02-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:31:09Z |
dc.date.available.fl_str_mv |
2016-01-24T14:31:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
General and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 182, p. 65-72, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/35893 http://dx.doi.org/10.1016/j.ygcen.2012.12.002 |
dc.identifier.issn.none.fl_str_mv |
0016-6480 |
dc.identifier.file.none.fl_str_mv |
WOS000315013100008.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.ygcen.2012.12.002 |
dc.identifier.wos.none.fl_str_mv |
WOS:000315013100008 |
identifier_str_mv |
General and Comparative Endocrinology. San Diego: Academic Press Inc Elsevier Science, v. 182, p. 65-72, 2013. 0016-6480 WOS000315013100008.pdf 10.1016/j.ygcen.2012.12.002 WOS:000315013100008 |
url |
http://repositorio.unifesp.br/handle/11600/35893 http://dx.doi.org/10.1016/j.ygcen.2012.12.002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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General and Comparative Endocrinology |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy info:eu-repo/semantics/openAccess |
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http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
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openAccess |
dc.format.none.fl_str_mv |
65-72 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
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Elsevier B.V. |
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