Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure

Detalhes bibliográficos
Autor(a) principal: Santos, Leonardo dos [UNIFESP]
Data de Publicação: 2010
Outros Autores: Antonio, Ednei L. [UNIFESP], Souza, Ana F. M. [UNIFESP], Tucci, Paulo J. F. [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1139/Y10-062
http://repositorio.unifesp.br/handle/11600/32651
Resumo: After myocardial infarction, the hemodynamics under basal conditions might appear to be unaltered, which makes it difficult to identify cardiac dysfunction by the usual approaches. Thus, we tested the response to sudden afterload stress in infarcted rats with apparently normal ejection function. Control (CT) and infarcted (MI) Wistar rats with various MI sizes were submitted to echocardiography 30 days after coronary occlusion, followed by assessment of hemodynamics under basal conditions and during a pharmacologically induced sudden pressure overload (phenylephrine 15-25 mg/kg, i.v.). Coronary occlusion resulted in cardiac remodeling proportional to MI size, although several functional parameters such as systolic pressure (SP), stroke volume (SV), and stroke work (SW) of all MI rats were similar to those of CT rats. However, the afterload stress that was produced led to a relative preservation of SV and an increase of SW in CT rats; MI rats exhibited a significant reduction in SV and SW generation, although global cardiac function was normal under basal conditions, as indicated by regular echocardiography and hemodynamics assessment. Thus, we propose the use of sudden pharmacologically induced afterload stress as a practical and efficient procedure for identifying impaired performance of the heart in anesthetized rats, providing an additional physiological variable to be evaluated in experimental therapeutic studies.
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spelling Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failurepressure overloadcardiac functionhemodynamic changesventricular pressureafterload mismatchphenylephrineratAfter myocardial infarction, the hemodynamics under basal conditions might appear to be unaltered, which makes it difficult to identify cardiac dysfunction by the usual approaches. Thus, we tested the response to sudden afterload stress in infarcted rats with apparently normal ejection function. Control (CT) and infarcted (MI) Wistar rats with various MI sizes were submitted to echocardiography 30 days after coronary occlusion, followed by assessment of hemodynamics under basal conditions and during a pharmacologically induced sudden pressure overload (phenylephrine 15-25 mg/kg, i.v.). Coronary occlusion resulted in cardiac remodeling proportional to MI size, although several functional parameters such as systolic pressure (SP), stroke volume (SV), and stroke work (SW) of all MI rats were similar to those of CT rats. However, the afterload stress that was produced led to a relative preservation of SV and an increase of SW in CT rats; MI rats exhibited a significant reduction in SV and SW generation, although global cardiac function was normal under basal conditions, as indicated by regular echocardiography and hemodynamics assessment. Thus, we propose the use of sudden pharmacologically induced afterload stress as a practical and efficient procedure for identifying impaired performance of the heart in anesthetized rats, providing an additional physiological variable to be evaluated in experimental therapeutic studies.Universidade Federal de São Paulo, Div Cardiol, São Paulo, BrazilUniversidade Federal de São Paulo, Cardioresp Physiol Div, São Paulo, BrazilUniversidade Federal de São Paulo, Div Cardiol, São Paulo, BrazilUniversidade Federal de São Paulo, Cardioresp Physiol Div, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Natl Research Council Canada-n R C Research PressUniversidade Federal de São Paulo (UNIFESP)Santos, Leonardo dos [UNIFESP]Antonio, Ednei L. [UNIFESP]Souza, Ana F. M. [UNIFESP]Tucci, Paulo J. F. [UNIFESP]2016-01-24T13:59:49Z2016-01-24T13:59:49Z2010-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion724-732http://dx.doi.org/10.1139/Y10-062Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 88, n. 7, p. 724-732, 2010.10.1139/Y10-0620008-4212http://repositorio.unifesp.br/handle/11600/32651WOS:000281209700004engCanadian Journal of Physiology and Pharmacologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-11-04T14:22:11Zoai:repositorio.unifesp.br/:11600/32651Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-11-04T14:22:11Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
title Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
spellingShingle Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
Santos, Leonardo dos [UNIFESP]
pressure overload
cardiac function
hemodynamic changes
ventricular pressure
afterload mismatch
phenylephrine
rat
title_short Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
title_full Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
title_fullStr Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
title_full_unstemmed Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
title_sort Use of afterload hemodynamic stress as a practical method for assessing cardiac performance in rats with heart failure
author Santos, Leonardo dos [UNIFESP]
author_facet Santos, Leonardo dos [UNIFESP]
Antonio, Ednei L. [UNIFESP]
Souza, Ana F. M. [UNIFESP]
Tucci, Paulo J. F. [UNIFESP]
author_role author
author2 Antonio, Ednei L. [UNIFESP]
Souza, Ana F. M. [UNIFESP]
Tucci, Paulo J. F. [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Santos, Leonardo dos [UNIFESP]
Antonio, Ednei L. [UNIFESP]
Souza, Ana F. M. [UNIFESP]
Tucci, Paulo J. F. [UNIFESP]
dc.subject.por.fl_str_mv pressure overload
cardiac function
hemodynamic changes
ventricular pressure
afterload mismatch
phenylephrine
rat
topic pressure overload
cardiac function
hemodynamic changes
ventricular pressure
afterload mismatch
phenylephrine
rat
description After myocardial infarction, the hemodynamics under basal conditions might appear to be unaltered, which makes it difficult to identify cardiac dysfunction by the usual approaches. Thus, we tested the response to sudden afterload stress in infarcted rats with apparently normal ejection function. Control (CT) and infarcted (MI) Wistar rats with various MI sizes were submitted to echocardiography 30 days after coronary occlusion, followed by assessment of hemodynamics under basal conditions and during a pharmacologically induced sudden pressure overload (phenylephrine 15-25 mg/kg, i.v.). Coronary occlusion resulted in cardiac remodeling proportional to MI size, although several functional parameters such as systolic pressure (SP), stroke volume (SV), and stroke work (SW) of all MI rats were similar to those of CT rats. However, the afterload stress that was produced led to a relative preservation of SV and an increase of SW in CT rats; MI rats exhibited a significant reduction in SV and SW generation, although global cardiac function was normal under basal conditions, as indicated by regular echocardiography and hemodynamics assessment. Thus, we propose the use of sudden pharmacologically induced afterload stress as a practical and efficient procedure for identifying impaired performance of the heart in anesthetized rats, providing an additional physiological variable to be evaluated in experimental therapeutic studies.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-01
2016-01-24T13:59:49Z
2016-01-24T13:59:49Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1139/Y10-062
Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 88, n. 7, p. 724-732, 2010.
10.1139/Y10-062
0008-4212
http://repositorio.unifesp.br/handle/11600/32651
WOS:000281209700004
url http://dx.doi.org/10.1139/Y10-062
http://repositorio.unifesp.br/handle/11600/32651
identifier_str_mv Canadian Journal of Physiology and Pharmacology. Ottawa: Natl Research Council Canada-n R C Research Press, v. 88, n. 7, p. 724-732, 2010.
10.1139/Y10-062
0008-4212
WOS:000281209700004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Canadian Journal of Physiology and Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 724-732
dc.publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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