Análise do perfil lipidômico em pacientes com câncer colorretal em estádios avançados

• Autor(a) principal: Figueiredo, Adiel Goes de [UNIFESP]
• Data de Publicação: 2017
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=5263782; http://repositorio.unifesp.br/handle/11600/50090
• topic: Lipidoma; Colorectal cancer; Lipids; Tumor biomarkers; Lipidoma; Cancer colorretal; Lipidios; Biomarcadores tumorais

Abstract In the last 10 years the incidence of colorectal cancer (CRC) has been increasing in developing countries. The search for molecular markers that reduce the need for invasive examinations for the diagnosis of colorectal cancer and the follow-up of treated patients advanced with the use of analytical technologies such as mass spectrometry (MS), which allowed the search for lipid metabolites as Candidates for probable biomarkers. The objective was to establish the lipid profile of patients with locally advanced, unresectable or metastatic colorectal cancer. Peripheral blood was collected from patients with colorectal cancer and patients with normal colonoscopy. After lipid extraction, the samples were processed and analyzed in the MALDI TOF / TOF equipment. From the data matrix, the statistical analyzes were performed by the principal component analysis methods and the least squares discriminant analysis. The importance of the variable in the projection was used to identify the ions that had the greatest discriminatory effect between the groups. Eight lipids were identified as potential biomarkers and a multiple logistic regression model was proposed to calculate the performance of the test where we observed values of AUC 0.87, sensitivity 88.33% and specificity 83.78% and for a validation test with 1000 permutations a p <0.001. The classes of lipids found were sphingolipids, glycerophospholipids and policetidia. The strength of the association between the peak intensities of these lipids and the presence of colorectal cancer make these metabolites candidates for possible biomarkers. We found that sphingolipid (m / z = 742.98869) could be a biomarker in monitoring patients with CRC. In the survival analysis, three lipids showed a prognostic value for colorectal cancer, sphingolipid (m / z = 857,11525) and policetidios (m / z = 876,20796) and glycerophospholipid (m / z = 1031.54773).