Gingko biloba leaves extract on growth and morphology of trypanosomatids

Detalhes bibliográficos
Autor(a) principal: Perazzo, Fabio Ferreira [UNIFESP]
Data de Publicação: 2011
Outros Autores: Venturelli, Sandra Mara, Silva, Paulo Marcio de Faria E., Soares, Maurlio Jose, Caputo, Lucelia Rita Gaudino, Schneedorf, Jose Maurcio, Fiorini, Joao Evangelista, Carvalho, Jose Carlos Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia
http://repositorio.unifesp.br/handle/11600/42866
Resumo: Gingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified.
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spelling Gingko biloba leaves extract on growth and morphology of trypanosomatidsEfecto del extracto de las hojas de Gingko biloba en el crecimiento y la morfología de tripanosomátidos.Gingko bilobahydroethanolic extracttrypanosomatidsHerpetomonas samuelpessoaiGingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified.Univ Fed Sao Paulo, Lab Farmacognosia, Sao Paulo, BrazilUniv Jose do Rosario Vellano, Lab Biol & Fisiol Microorganismos, BR-37130000 Alfenas, MG, BrazilUniv Fed Alfenas, Microbiol Lab, Fac Farm, Alfenas, MG, BrazilFiocruz MS, Dept Ultra Estrutura & Biol, Inst Oswaldo Cruz, BR-21045900 Rio De Janeiro, BrazilUniv Fed Amapa, Lab Pesquisa Farmacos, Ctr Ciencias Biol & Saude, BR-68902280 Macapa, Amapa, BrazilUniv Fed Sao Paulo, Lab Farmacognosia, Sao Paulo, BrazilWeb of ScienceSoc Fitoquimica LatinoamericanaUniversidade Federal de São Paulo (UNIFESP)Univ Jose do Rosario VellanoUniv Fed AlfenasFiocruz MSUniv Fed AmapaPerazzo, Fabio Ferreira [UNIFESP]Venturelli, Sandra MaraSilva, Paulo Marcio de Faria E.Soares, Maurlio JoseCaputo, Lucelia Rita GaudinoSchneedorf, Jose MaurcioFiorini, Joao EvangelistaCarvalho, Jose Carlos Tavares2018-06-15T14:04:30Z2018-06-15T14:04:30Z2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion147-154application/pdfhttp://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologiaBoletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011.WOS000291640700007.pdf0717-7917http://repositorio.unifesp.br/handle/11600/42866WOS:000291640700007engBoletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-09T04:53:59Zoai:repositorio.unifesp.br/:11600/42866Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-09T04:53:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Gingko biloba leaves extract on growth and morphology of trypanosomatids
Efecto del extracto de las hojas de Gingko biloba en el crecimiento y la morfología de tripanosomátidos.
title Gingko biloba leaves extract on growth and morphology of trypanosomatids
spellingShingle Gingko biloba leaves extract on growth and morphology of trypanosomatids
Perazzo, Fabio Ferreira [UNIFESP]
Gingko biloba
hydroethanolic extract
trypanosomatids
Herpetomonas samuelpessoai
title_short Gingko biloba leaves extract on growth and morphology of trypanosomatids
title_full Gingko biloba leaves extract on growth and morphology of trypanosomatids
title_fullStr Gingko biloba leaves extract on growth and morphology of trypanosomatids
title_full_unstemmed Gingko biloba leaves extract on growth and morphology of trypanosomatids
title_sort Gingko biloba leaves extract on growth and morphology of trypanosomatids
author Perazzo, Fabio Ferreira [UNIFESP]
author_facet Perazzo, Fabio Ferreira [UNIFESP]
Venturelli, Sandra Mara
Silva, Paulo Marcio de Faria E.
Soares, Maurlio Jose
Caputo, Lucelia Rita Gaudino
Schneedorf, Jose Maurcio
Fiorini, Joao Evangelista
Carvalho, Jose Carlos Tavares
author_role author
author2 Venturelli, Sandra Mara
Silva, Paulo Marcio de Faria E.
Soares, Maurlio Jose
Caputo, Lucelia Rita Gaudino
Schneedorf, Jose Maurcio
Fiorini, Joao Evangelista
Carvalho, Jose Carlos Tavares
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Univ Jose do Rosario Vellano
Univ Fed Alfenas
Fiocruz MS
Univ Fed Amapa
dc.contributor.author.fl_str_mv Perazzo, Fabio Ferreira [UNIFESP]
Venturelli, Sandra Mara
Silva, Paulo Marcio de Faria E.
Soares, Maurlio Jose
Caputo, Lucelia Rita Gaudino
Schneedorf, Jose Maurcio
Fiorini, Joao Evangelista
Carvalho, Jose Carlos Tavares
dc.subject.por.fl_str_mv Gingko biloba
hydroethanolic extract
trypanosomatids
Herpetomonas samuelpessoai
topic Gingko biloba
hydroethanolic extract
trypanosomatids
Herpetomonas samuelpessoai
description Gingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified.
publishDate 2011
dc.date.none.fl_str_mv 2011-03-01
2018-06-15T14:04:30Z
2018-06-15T14:04:30Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia
Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011.
WOS000291640700007.pdf
0717-7917
http://repositorio.unifesp.br/handle/11600/42866
WOS:000291640700007
url http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia
http://repositorio.unifesp.br/handle/11600/42866
identifier_str_mv Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011.
WOS000291640700007.pdf
0717-7917
WOS:000291640700007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-154
application/pdf
dc.publisher.none.fl_str_mv Soc Fitoquimica Latinoamericana
publisher.none.fl_str_mv Soc Fitoquimica Latinoamericana
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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