Gingko biloba leaves extract on growth and morphology of trypanosomatids
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia http://repositorio.unifesp.br/handle/11600/42866 |
Resumo: | Gingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified. |
id |
UFSP_db07f86918b57299dff43edf7a0adf59 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/42866 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Gingko biloba leaves extract on growth and morphology of trypanosomatidsEfecto del extracto de las hojas de Gingko biloba en el crecimiento y la morfología de tripanosomátidos.Gingko bilobahydroethanolic extracttrypanosomatidsHerpetomonas samuelpessoaiGingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified.Univ Fed Sao Paulo, Lab Farmacognosia, Sao Paulo, BrazilUniv Jose do Rosario Vellano, Lab Biol & Fisiol Microorganismos, BR-37130000 Alfenas, MG, BrazilUniv Fed Alfenas, Microbiol Lab, Fac Farm, Alfenas, MG, BrazilFiocruz MS, Dept Ultra Estrutura & Biol, Inst Oswaldo Cruz, BR-21045900 Rio De Janeiro, BrazilUniv Fed Amapa, Lab Pesquisa Farmacos, Ctr Ciencias Biol & Saude, BR-68902280 Macapa, Amapa, BrazilUniv Fed Sao Paulo, Lab Farmacognosia, Sao Paulo, BrazilWeb of ScienceSoc Fitoquimica LatinoamericanaUniversidade Federal de São Paulo (UNIFESP)Univ Jose do Rosario VellanoUniv Fed AlfenasFiocruz MSUniv Fed AmapaPerazzo, Fabio Ferreira [UNIFESP]Venturelli, Sandra MaraSilva, Paulo Marcio de Faria E.Soares, Maurlio JoseCaputo, Lucelia Rita GaudinoSchneedorf, Jose MaurcioFiorini, Joao EvangelistaCarvalho, Jose Carlos Tavares2018-06-15T14:04:30Z2018-06-15T14:04:30Z2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion147-154application/pdfhttp://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologiaBoletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011.WOS000291640700007.pdf0717-7917http://repositorio.unifesp.br/handle/11600/42866WOS:000291640700007engBoletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-09T04:53:59Zoai:repositorio.unifesp.br/:11600/42866Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-09T04:53:59Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Gingko biloba leaves extract on growth and morphology of trypanosomatids Efecto del extracto de las hojas de Gingko biloba en el crecimiento y la morfología de tripanosomátidos. |
title |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
spellingShingle |
Gingko biloba leaves extract on growth and morphology of trypanosomatids Perazzo, Fabio Ferreira [UNIFESP] Gingko biloba hydroethanolic extract trypanosomatids Herpetomonas samuelpessoai |
title_short |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
title_full |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
title_fullStr |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
title_full_unstemmed |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
title_sort |
Gingko biloba leaves extract on growth and morphology of trypanosomatids |
author |
Perazzo, Fabio Ferreira [UNIFESP] |
author_facet |
Perazzo, Fabio Ferreira [UNIFESP] Venturelli, Sandra Mara Silva, Paulo Marcio de Faria E. Soares, Maurlio Jose Caputo, Lucelia Rita Gaudino Schneedorf, Jose Maurcio Fiorini, Joao Evangelista Carvalho, Jose Carlos Tavares |
author_role |
author |
author2 |
Venturelli, Sandra Mara Silva, Paulo Marcio de Faria E. Soares, Maurlio Jose Caputo, Lucelia Rita Gaudino Schneedorf, Jose Maurcio Fiorini, Joao Evangelista Carvalho, Jose Carlos Tavares |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Jose do Rosario Vellano Univ Fed Alfenas Fiocruz MS Univ Fed Amapa |
dc.contributor.author.fl_str_mv |
Perazzo, Fabio Ferreira [UNIFESP] Venturelli, Sandra Mara Silva, Paulo Marcio de Faria E. Soares, Maurlio Jose Caputo, Lucelia Rita Gaudino Schneedorf, Jose Maurcio Fiorini, Joao Evangelista Carvalho, Jose Carlos Tavares |
dc.subject.por.fl_str_mv |
Gingko biloba hydroethanolic extract trypanosomatids Herpetomonas samuelpessoai |
topic |
Gingko biloba hydroethanolic extract trypanosomatids Herpetomonas samuelpessoai |
description |
Gingko biloba has been one of the most used medicinal plants all over the world in the past years. In this study, our group has studied the effect of a hydroethanolic extract from the aerial parts of this plant on the growth and morphological differentiation of trypanosomatids. Herpetomonas samuelpessoai and Herpetomonas sp were used in this study. The extract was obtained in a Soxhlet apparatus (50 degrees C, 2 hours). This extract was aseptically added to Roitman's medium in different concentrations (4, 20, 40, 60, 80 and 100 mg/ml). The growth rate was determined using a Newbauer chamber to count numbers of cells after the extract inoculation (24 and 72 hours later). Smears stained by the Panotic method was used to determine the percentages of pro, para and opisthomastigote forms. The extract inhibited Herpetomonas sp growth in concentrations higher than 20 mg/ml. H. samuelpessoai has been inhibited in doses higher than 40 mg/ml. No morphological differentiation was observed in Herpetomonas sp cell. However, morphological differentiations could be noticed in H. samuelpessoai cell using doses higher than 40 mg/ml. These alterations are probably related to the cell division process, since cells with 3 or 4 nucleus were observed. Also, cytoplasmatic expansions, representing unsuccessful process of cell division were frequently found out. Further ultrastructural analysis using a transmission electron microscope showed cells with homogeneous nucleus or the absence of it. Protozoan protein profile was also analyzed. It was possible to notice changes in both trypanosomatids used in this study. H. samuelpessoai has shown over expression and accumulation of proteins which its degradation is essential to continue the cell differentiation. Also, it is possible to suggest that this extract acts through the modulation of the genetic expression and may be harmful to human cells if not purified. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2018-06-15T14:04:30Z 2018-06-15T14:04:30Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011. WOS000291640700007.pdf 0717-7917 http://repositorio.unifesp.br/handle/11600/42866 WOS:000291640700007 |
url |
http://www.blacpma.usach.cl/revista-numero/efecto-del-extracto-de-las-hojas-de-gingko-biloba-en-el-crecimiento-y-la-morfologia http://repositorio.unifesp.br/handle/11600/42866 |
identifier_str_mv |
Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas. Santiago 7: Soc Fitoquimica Latinoamericana, v. 10, n. 2, p. 147-154, 2011. WOS000291640700007.pdf 0717-7917 WOS:000291640700007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
147-154 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Fitoquimica Latinoamericana |
publisher.none.fl_str_mv |
Soc Fitoquimica Latinoamericana |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1824718234113277952 |