The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener

Detalhes bibliográficos
Autor(a) principal: Santos, Marcia Regina Machado [UNIFESP]
Data de Publicação: 1997
Outros Autores: Cano, Maria I [UNIFESP], Schijman, Alejandro, Lorenzi, Hernan, Vázquez, Martin, Levin, Mariano J, Ramirez, José Luis, Brandão, Adeílton, Degrave, Wim M, Franco da Silveira, José [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://repositorio.unifesp.br/handle/11600/544
http://dx.doi.org/10.1590/S0074-02761997000600018
Resumo: By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes
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spelling Santos, Marcia Regina Machado [UNIFESP]Cano, Maria I [UNIFESP]Schijman, AlejandroLorenzi, HernanVázquez, MartinLevin, Mariano JRamirez, José LuisBrandão, AdeíltonDegrave, Wim MFranco da Silveira, José [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Instituto de Investigaciones en Ingenieria Genetica y Biologia MolecularUniversidad Central de VenezuelaInstituto Oswaldo Cruz2015-06-14T13:24:39Z2015-06-14T13:24:39Z1997-11-01Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.0074-0276http://repositorio.unifesp.br/handle/11600/544http://dx.doi.org/10.1590/S0074-02761997000600018S0074-02761997000600018.pdfS0074-0276199700060001810.1590/S0074-02761997000600018By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizesUniversidade Federal de São Paulo (UNIFESP)Instituto de Investigaciones en Ingenieria Genetica y Biologia MolecularUniversidad Central de VenezuelaInstituto Oswaldo CruzUNIFESPSciELO821-828engInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo CruzTrypanosoma cruziclone CLBrenergenome projectmolecular karyotypechromosome mappinggenetic mapThe Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brenerinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0074-02761997000600018.pdfapplication/pdf157316${dspace.ui.url}/bitstream/11600/544/1/S0074-02761997000600018.pdf0e2a2ef0affe4cd0a69dcbbf1b53d7deMD51open accessTEXTS0074-02761997000600018.pdf.txtS0074-02761997000600018.pdf.txtExtracted texttext/plain33202${dspace.ui.url}/bitstream/11600/544/2/S0074-02761997000600018.pdf.txtd93aa440058df26654e493d34edff912MD52open access11600/5442015-08-30 11:42:24.676open accessoai:repositorio.unifesp.br:11600/544Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652015-08-30T14:42:24Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.en.fl_str_mv The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
spellingShingle The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
Santos, Marcia Regina Machado [UNIFESP]
Trypanosoma cruzi
clone CL
Brener
genome project
molecular karyotype
chromosome mapping
genetic map
title_short The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_fullStr The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_full_unstemmed The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
title_sort The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
author Santos, Marcia Regina Machado [UNIFESP]
author_facet Santos, Marcia Regina Machado [UNIFESP]
Cano, Maria I [UNIFESP]
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J
Ramirez, José Luis
Brandão, Adeílton
Degrave, Wim M
Franco da Silveira, José [UNIFESP]
author_role author
author2 Cano, Maria I [UNIFESP]
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J
Ramirez, José Luis
Brandão, Adeílton
Degrave, Wim M
Franco da Silveira, José [UNIFESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.institution.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular
Universidad Central de Venezuela
Instituto Oswaldo Cruz
dc.contributor.author.fl_str_mv Santos, Marcia Regina Machado [UNIFESP]
Cano, Maria I [UNIFESP]
Schijman, Alejandro
Lorenzi, Hernan
Vázquez, Martin
Levin, Mariano J
Ramirez, José Luis
Brandão, Adeílton
Degrave, Wim M
Franco da Silveira, José [UNIFESP]
dc.subject.eng.fl_str_mv Trypanosoma cruzi
clone CL
Brener
genome project
molecular karyotype
chromosome mapping
genetic map
topic Trypanosoma cruzi
clone CL
Brener
genome project
molecular karyotype
chromosome mapping
genetic map
description By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes
publishDate 1997
dc.date.issued.fl_str_mv 1997-11-01
dc.date.accessioned.fl_str_mv 2015-06-14T13:24:39Z
dc.date.available.fl_str_mv 2015-06-14T13:24:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.
dc.identifier.uri.fl_str_mv http://repositorio.unifesp.br/handle/11600/544
http://dx.doi.org/10.1590/S0074-02761997000600018
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.file.none.fl_str_mv S0074-02761997000600018.pdf
dc.identifier.scielo.none.fl_str_mv S0074-02761997000600018
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761997000600018
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 92, n. 6, p. 821-828, 1997.
0074-0276
S0074-02761997000600018.pdf
S0074-02761997000600018
10.1590/S0074-02761997000600018
url http://repositorio.unifesp.br/handle/11600/544
http://dx.doi.org/10.1590/S0074-02761997000600018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 821-828
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
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