Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/0013000011q1f |
| DOI: | 10.1128/JCM.02478-12 |
| Texto Completo: | http://dx.doi.org/10.1128/JCM.02478-12 http://repositorio.unifesp.br/handle/11600/36006 |
Resumo: | A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea. |
| id |
UFSP_ded12376644fd5bc53f31a9b628a0aec |
|---|---|
| oai_identifier_str |
oai:repositorio.unifesp.br/:11600/36006 |
| network_acronym_str |
UFSP |
| network_name_str |
Repositório Institucional da UNIFESP |
| repository_id_str |
3465 |
| spelling |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coliA real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea.Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, São Paulo, BrazilUniv Fed Espirito Santo, Dept Patol, Espirito Santo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Reumatol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Amer Soc MicrobiologyUniversidade Federal de São Paulo (UNIFESP)Univ Fed Espirito SantoSouza, Tamara B. [UNIFESP]Lozer, Diego M.Kitagawa, Sonia M. S.Spano, Liliana C.Silva, Neusa P. [UNIFESP]Scaletsky, Isabel C. A. [UNIFESP]2016-01-24T14:31:18Z2016-01-24T14:31:18Z2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1031-1033application/pdfhttp://dx.doi.org/10.1128/JCM.02478-12Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 51, n. 3, p. 1031-1033, 2013.10.1128/JCM.02478-12WOS000315121700052.pdf0095-1137http://repositorio.unifesp.br/handle/11600/36006WOS:000315121700052ark:/48912/0013000011q1fengJournal of Clinical Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T19:29:46Zoai:repositorio.unifesp.br/:11600/36006Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:50:07.188043Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| title |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| spellingShingle |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli Souza, Tamara B. [UNIFESP] Souza, Tamara B. [UNIFESP] |
| title_short |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| title_full |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| title_fullStr |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| title_full_unstemmed |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| title_sort |
Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli |
| author |
Souza, Tamara B. [UNIFESP] |
| author_facet |
Souza, Tamara B. [UNIFESP] Souza, Tamara B. [UNIFESP] Lozer, Diego M. Kitagawa, Sonia M. S. Spano, Liliana C. Silva, Neusa P. [UNIFESP] Scaletsky, Isabel C. A. [UNIFESP] Lozer, Diego M. Kitagawa, Sonia M. S. Spano, Liliana C. Silva, Neusa P. [UNIFESP] Scaletsky, Isabel C. A. [UNIFESP] |
| author_role |
author |
| author2 |
Lozer, Diego M. Kitagawa, Sonia M. S. Spano, Liliana C. Silva, Neusa P. [UNIFESP] Scaletsky, Isabel C. A. [UNIFESP] |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Univ Fed Espirito Santo |
| dc.contributor.author.fl_str_mv |
Souza, Tamara B. [UNIFESP] Lozer, Diego M. Kitagawa, Sonia M. S. Spano, Liliana C. Silva, Neusa P. [UNIFESP] Scaletsky, Isabel C. A. [UNIFESP] |
| description |
A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagenic E. coli isolates from children with diarrhea. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-03-01 2016-01-24T14:31:18Z 2016-01-24T14:31:18Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/JCM.02478-12 Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 51, n. 3, p. 1031-1033, 2013. 10.1128/JCM.02478-12 WOS000315121700052.pdf 0095-1137 http://repositorio.unifesp.br/handle/11600/36006 WOS:000315121700052 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/0013000011q1f |
| url |
http://dx.doi.org/10.1128/JCM.02478-12 http://repositorio.unifesp.br/handle/11600/36006 |
| identifier_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 51, n. 3, p. 1031-1033, 2013. 10.1128/JCM.02478-12 WOS000315121700052.pdf 0095-1137 WOS:000315121700052 ark:/48912/0013000011q1f |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Clinical Microbiology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
1031-1033 application/pdf |
| dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
| publisher.none.fl_str_mv |
Amer Soc Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1822251239270776832 |
| dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.02478-12 |