The buccal mucosa fenestrated graft for Bracka first stage urethroplasty: experimental study in rabbits

• Autor(a) principal: Oliva, Petrus
• Data de Publicação: 2012
• Outros Autores: Delcelo, Rosana [UNIFESP], Bacelar, Herick, Rondon, Atila, Barroso Jr., Ubirajara, Ortiz, Valdemar [UNIFESP], Macedo Jr., Antônio
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• dARK ID: ark:/48912/0013000015p7s
• DOI: 10.1590/1677-553820133806825
• Texto Completo: http://dx.doi.org/10.1590/1677-553820133806825; http://repositorio.unifesp.br/handle/11600/7458
• topic: Hypospadias; Surgery; Mouth Mucosa, Transplantation; Urethra; Models, Animal

Objective To histologically evaluate, in an experimental study in rabbits, the integration process of the buccal mucosa fenestrated graft applied in the corpora cavernosa for Bracka first stage urethroplasty. Materials and Methods A urethral defect was surgically created in 16 male rabbits of the New Zealand breed through the excision of the penile urethra. The urethral defect was corrected by applying buccal mucosa fenestrated graft through two cruciform incisions in the distal portions of its longitudinal axis. The animals were sacrificed at 2, 4, 8 and 12 weeks post surgery and their genitals were subjected to clinical and histological assessment. Results The buccal mucosa fenestrated graft showed complete uptake in all groups, with keratinization squamous metaplasia and mucosal proliferation of the fenestrated areas. The fenestrated graft area represented an increase in length of 25% in length in relation to the original standard graft. Conclusions The fenestrated buccal mucosa graft presented total integration to the adjacent epithelia with re-epithelization of the incision areas of the graft (fenestrations) and no significant inflammatory or scarring reactions when compared to other mucosa transplanted areas; therefore its application is viable in cases of extensive urethral defect whenever the donating area might be insufficient.