Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis

Detalhes bibliográficos
Autor(a) principal: Desjardins, Christopher A.
Data de Publicação: 2011
Outros Autores: Champion, Mia D., Holder, Jason W., Muszewska, Anna, Goldberg, Jonathan, Bailao, Alexandre M., Brigido, Marcelo Macedo, Silva Ferreira, Marcia Eliana da, Garcia, Ana Maria, Grynberg, Marcin, Gujja, Sharvari, Heiman, David I., Henn, Matthew R., Kodira, Chinnappa D., Leon-Narvaez, Henry, Longo, Larissa V. G. [UNIFESP], Ma, Li-Jun, Malavazi, Iran, Matsuo, Alisson L. [UNIFESP], Morais, Flavia V. [UNIFESP], Pereira, Maristela, Rodriguez-Brito, Sabrina, Sakthikumar, Sharadha, Salem-Izacc, Silvia M., Sykes, Sean M., Teixeira, Marcus Melo, Vallejo, Milene C. [UNIFESP], Machado Telles Walter, Maria Emilia, Yandava, Chandri, Young, Sarah, Zeng, Qiandong, Zucker, Jeremy, Felipe, Maria Sueli, Goldman, Gustavo H., Haas, Brian J., McEwen, Juan G., Nino-Vega, Gustavo, Puccia, Rosana [UNIFESP], San-Blas, Gioconda, Almeida Soares, Celia Maria de, Birren, Bruce W., Cuomo, Christina A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pgen.1002345
http://repositorio.unifesp.br/handle/11600/34080
Resumo: Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.
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spelling Comparative Genomic Analysis of Human Fungal Pathogens Causing ParacoccidioidomycosisParacoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.Broad Inst MIT & Harvard, Cambridge, MA USAMIT, Dept Biol, Cambridge, MA USAPolish Acad Sci, Inst Biochem & Biophys, Warsaw, PolandUniv Fed Goias, Mol Biol Lab, Inst Ciencias Biol, Goiania, Go, BrazilUniv Brasilia, Inst Ciencias Biol, Brasilia, DF, BrazilUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, BrazilCorp Invest Biol, Unidad Biol Celular & Mol, Medellin, ColombiaInst Venezolano Invest Cient, Ctr Microbiol & Biol Celular, Caracas, VenezuelaUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, BrazilUniv Brasilia, Inst Ciencias Exatas, Brasilia, DF, BrazilLab Nacl Ciencia & Tecnol Bioetanol CTBE, São Paulo, BrazilUniv Antioquia, Fac Med, Medellin, ColombiaUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilWeb of ScienceNational Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human ServicesConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services: HHSN266200400001CNational Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services: HHSN2722009000018CPublic Library ScienceBroad Inst MIT & HarvardMITPolish Acad SciUniversidade Federal de Goiás (UFG)Universidade de Brasília (UnB)Universidade de São Paulo (USP)Corp Invest BiolInst Venezolano Invest CientUniversidade Federal de São Paulo (UNIFESP)Univ Vale ParaibaLab Nacl Ciencia & Tecnol Bioetanol CTBEUniv AntioquiaDesjardins, Christopher A.Champion, Mia D.Holder, Jason W.Muszewska, AnnaGoldberg, JonathanBailao, Alexandre M.Brigido, Marcelo MacedoSilva Ferreira, Marcia Eliana daGarcia, Ana MariaGrynberg, MarcinGujja, SharvariHeiman, David I.Henn, Matthew R.Kodira, Chinnappa D.Leon-Narvaez, HenryLongo, Larissa V. G. [UNIFESP]Ma, Li-JunMalavazi, IranMatsuo, Alisson L. [UNIFESP]Morais, Flavia V. [UNIFESP]Pereira, MaristelaRodriguez-Brito, SabrinaSakthikumar, SharadhaSalem-Izacc, Silvia M.Sykes, Sean M.Teixeira, Marcus MeloVallejo, Milene C. [UNIFESP]Machado Telles Walter, Maria EmiliaYandava, ChandriYoung, SarahZeng, QiandongZucker, JeremyFelipe, Maria SueliGoldman, Gustavo H.Haas, Brian J.McEwen, Juan G.Nino-Vega, GustavoPuccia, Rosana [UNIFESP]San-Blas, GiocondaAlmeida Soares, Celia Maria deBirren, Bruce W.Cuomo, Christina A.2016-01-24T14:17:15Z2016-01-24T14:17:15Z2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16application/pdfhttp://dx.doi.org/10.1371/journal.pgen.1002345Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011.10.1371/journal.pgen.1002345WOS000296665400043.pdf1553-7404http://repositorio.unifesp.br/handle/11600/34080WOS:000296665400043engPlos Geneticsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T21:29:50Zoai:repositorio.unifesp.br/:11600/34080Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T21:29:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
title Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
spellingShingle Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
Desjardins, Christopher A.
title_short Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
title_full Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
title_fullStr Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
title_full_unstemmed Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
title_sort Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
author Desjardins, Christopher A.
author_facet Desjardins, Christopher A.
Champion, Mia D.
Holder, Jason W.
Muszewska, Anna
Goldberg, Jonathan
Bailao, Alexandre M.
Brigido, Marcelo Macedo
Silva Ferreira, Marcia Eliana da
Garcia, Ana Maria
Grynberg, Marcin
Gujja, Sharvari
Heiman, David I.
Henn, Matthew R.
Kodira, Chinnappa D.
Leon-Narvaez, Henry
Longo, Larissa V. G. [UNIFESP]
Ma, Li-Jun
Malavazi, Iran
Matsuo, Alisson L. [UNIFESP]
Morais, Flavia V. [UNIFESP]
Pereira, Maristela
Rodriguez-Brito, Sabrina
Sakthikumar, Sharadha
Salem-Izacc, Silvia M.
Sykes, Sean M.
Teixeira, Marcus Melo
Vallejo, Milene C. [UNIFESP]
Machado Telles Walter, Maria Emilia
Yandava, Chandri
Young, Sarah
Zeng, Qiandong
Zucker, Jeremy
Felipe, Maria Sueli
Goldman, Gustavo H.
Haas, Brian J.
McEwen, Juan G.
Nino-Vega, Gustavo
Puccia, Rosana [UNIFESP]
San-Blas, Gioconda
Almeida Soares, Celia Maria de
Birren, Bruce W.
Cuomo, Christina A.
author_role author
author2 Champion, Mia D.
Holder, Jason W.
Muszewska, Anna
Goldberg, Jonathan
Bailao, Alexandre M.
Brigido, Marcelo Macedo
Silva Ferreira, Marcia Eliana da
Garcia, Ana Maria
Grynberg, Marcin
Gujja, Sharvari
Heiman, David I.
Henn, Matthew R.
Kodira, Chinnappa D.
Leon-Narvaez, Henry
Longo, Larissa V. G. [UNIFESP]
Ma, Li-Jun
Malavazi, Iran
Matsuo, Alisson L. [UNIFESP]
Morais, Flavia V. [UNIFESP]
Pereira, Maristela
Rodriguez-Brito, Sabrina
Sakthikumar, Sharadha
Salem-Izacc, Silvia M.
Sykes, Sean M.
Teixeira, Marcus Melo
Vallejo, Milene C. [UNIFESP]
Machado Telles Walter, Maria Emilia
Yandava, Chandri
Young, Sarah
Zeng, Qiandong
Zucker, Jeremy
Felipe, Maria Sueli
Goldman, Gustavo H.
Haas, Brian J.
McEwen, Juan G.
Nino-Vega, Gustavo
Puccia, Rosana [UNIFESP]
San-Blas, Gioconda
Almeida Soares, Celia Maria de
Birren, Bruce W.
Cuomo, Christina A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Broad Inst MIT & Harvard
MIT
Polish Acad Sci
Universidade Federal de Goiás (UFG)
Universidade de Brasília (UnB)
Universidade de São Paulo (USP)
Corp Invest Biol
Inst Venezolano Invest Cient
Universidade Federal de São Paulo (UNIFESP)
Univ Vale Paraiba
Lab Nacl Ciencia & Tecnol Bioetanol CTBE
Univ Antioquia
dc.contributor.author.fl_str_mv Desjardins, Christopher A.
Champion, Mia D.
Holder, Jason W.
Muszewska, Anna
Goldberg, Jonathan
Bailao, Alexandre M.
Brigido, Marcelo Macedo
Silva Ferreira, Marcia Eliana da
Garcia, Ana Maria
Grynberg, Marcin
Gujja, Sharvari
Heiman, David I.
Henn, Matthew R.
Kodira, Chinnappa D.
Leon-Narvaez, Henry
Longo, Larissa V. G. [UNIFESP]
Ma, Li-Jun
Malavazi, Iran
Matsuo, Alisson L. [UNIFESP]
Morais, Flavia V. [UNIFESP]
Pereira, Maristela
Rodriguez-Brito, Sabrina
Sakthikumar, Sharadha
Salem-Izacc, Silvia M.
Sykes, Sean M.
Teixeira, Marcus Melo
Vallejo, Milene C. [UNIFESP]
Machado Telles Walter, Maria Emilia
Yandava, Chandri
Young, Sarah
Zeng, Qiandong
Zucker, Jeremy
Felipe, Maria Sueli
Goldman, Gustavo H.
Haas, Brian J.
McEwen, Juan G.
Nino-Vega, Gustavo
Puccia, Rosana [UNIFESP]
San-Blas, Gioconda
Almeida Soares, Celia Maria de
Birren, Bruce W.
Cuomo, Christina A.
description Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
2016-01-24T14:17:15Z
2016-01-24T14:17:15Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pgen.1002345
Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011.
10.1371/journal.pgen.1002345
WOS000296665400043.pdf
1553-7404
http://repositorio.unifesp.br/handle/11600/34080
WOS:000296665400043
url http://dx.doi.org/10.1371/journal.pgen.1002345
http://repositorio.unifesp.br/handle/11600/34080
identifier_str_mv Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011.
10.1371/journal.pgen.1002345
WOS000296665400043.pdf
1553-7404
WOS:000296665400043
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos Genetics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16
application/pdf
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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