Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pgen.1002345 http://repositorio.unifesp.br/handle/11600/34080 |
Resumo: | Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts. |
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Comparative Genomic Analysis of Human Fungal Pathogens Causing ParacoccidioidomycosisParacoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.Broad Inst MIT & Harvard, Cambridge, MA USAMIT, Dept Biol, Cambridge, MA USAPolish Acad Sci, Inst Biochem & Biophys, Warsaw, PolandUniv Fed Goias, Mol Biol Lab, Inst Ciencias Biol, Goiania, Go, BrazilUniv Brasilia, Inst Ciencias Biol, Brasilia, DF, BrazilUniv São Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, BrazilCorp Invest Biol, Unidad Biol Celular & Mol, Medellin, ColombiaInst Venezolano Invest Cient, Ctr Microbiol & Biol Celular, Caracas, VenezuelaUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, BrazilUniv Brasilia, Inst Ciencias Exatas, Brasilia, DF, BrazilLab Nacl Ciencia & Tecnol Bioetanol CTBE, São Paulo, BrazilUniv Antioquia, Fac Med, Medellin, ColombiaUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, BrazilWeb of ScienceNational Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human ServicesConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services: HHSN266200400001CNational Institute of Allergy and Infectious Disease, National Institutes of Health, Department of Health and Human Services: HHSN2722009000018CPublic Library ScienceBroad Inst MIT & HarvardMITPolish Acad SciUniversidade Federal de Goiás (UFG)Universidade de Brasília (UnB)Universidade de São Paulo (USP)Corp Invest BiolInst Venezolano Invest CientUniversidade Federal de São Paulo (UNIFESP)Univ Vale ParaibaLab Nacl Ciencia & Tecnol Bioetanol CTBEUniv AntioquiaDesjardins, Christopher A.Champion, Mia D.Holder, Jason W.Muszewska, AnnaGoldberg, JonathanBailao, Alexandre M.Brigido, Marcelo MacedoSilva Ferreira, Marcia Eliana daGarcia, Ana MariaGrynberg, MarcinGujja, SharvariHeiman, David I.Henn, Matthew R.Kodira, Chinnappa D.Leon-Narvaez, HenryLongo, Larissa V. G. [UNIFESP]Ma, Li-JunMalavazi, IranMatsuo, Alisson L. [UNIFESP]Morais, Flavia V. [UNIFESP]Pereira, MaristelaRodriguez-Brito, SabrinaSakthikumar, SharadhaSalem-Izacc, Silvia M.Sykes, Sean M.Teixeira, Marcus MeloVallejo, Milene C. [UNIFESP]Machado Telles Walter, Maria EmiliaYandava, ChandriYoung, SarahZeng, QiandongZucker, JeremyFelipe, Maria SueliGoldman, Gustavo H.Haas, Brian J.McEwen, Juan G.Nino-Vega, GustavoPuccia, Rosana [UNIFESP]San-Blas, GiocondaAlmeida Soares, Celia Maria deBirren, Bruce W.Cuomo, Christina A.2016-01-24T14:17:15Z2016-01-24T14:17:15Z2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16application/pdfhttp://dx.doi.org/10.1371/journal.pgen.1002345Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011.10.1371/journal.pgen.1002345WOS000296665400043.pdf1553-7404http://repositorio.unifesp.br/handle/11600/34080WOS:000296665400043engPlos Geneticsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T21:29:50Zoai:repositorio.unifesp.br/:11600/34080Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T21:29:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
title |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
spellingShingle |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis Desjardins, Christopher A. |
title_short |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
title_full |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
title_fullStr |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
title_full_unstemmed |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
title_sort |
Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis |
author |
Desjardins, Christopher A. |
author_facet |
Desjardins, Christopher A. Champion, Mia D. Holder, Jason W. Muszewska, Anna Goldberg, Jonathan Bailao, Alexandre M. Brigido, Marcelo Macedo Silva Ferreira, Marcia Eliana da Garcia, Ana Maria Grynberg, Marcin Gujja, Sharvari Heiman, David I. Henn, Matthew R. Kodira, Chinnappa D. Leon-Narvaez, Henry Longo, Larissa V. G. [UNIFESP] Ma, Li-Jun Malavazi, Iran Matsuo, Alisson L. [UNIFESP] Morais, Flavia V. [UNIFESP] Pereira, Maristela Rodriguez-Brito, Sabrina Sakthikumar, Sharadha Salem-Izacc, Silvia M. Sykes, Sean M. Teixeira, Marcus Melo Vallejo, Milene C. [UNIFESP] Machado Telles Walter, Maria Emilia Yandava, Chandri Young, Sarah Zeng, Qiandong Zucker, Jeremy Felipe, Maria Sueli Goldman, Gustavo H. Haas, Brian J. McEwen, Juan G. Nino-Vega, Gustavo Puccia, Rosana [UNIFESP] San-Blas, Gioconda Almeida Soares, Celia Maria de Birren, Bruce W. Cuomo, Christina A. |
author_role |
author |
author2 |
Champion, Mia D. Holder, Jason W. Muszewska, Anna Goldberg, Jonathan Bailao, Alexandre M. Brigido, Marcelo Macedo Silva Ferreira, Marcia Eliana da Garcia, Ana Maria Grynberg, Marcin Gujja, Sharvari Heiman, David I. Henn, Matthew R. Kodira, Chinnappa D. Leon-Narvaez, Henry Longo, Larissa V. G. [UNIFESP] Ma, Li-Jun Malavazi, Iran Matsuo, Alisson L. [UNIFESP] Morais, Flavia V. [UNIFESP] Pereira, Maristela Rodriguez-Brito, Sabrina Sakthikumar, Sharadha Salem-Izacc, Silvia M. Sykes, Sean M. Teixeira, Marcus Melo Vallejo, Milene C. [UNIFESP] Machado Telles Walter, Maria Emilia Yandava, Chandri Young, Sarah Zeng, Qiandong Zucker, Jeremy Felipe, Maria Sueli Goldman, Gustavo H. Haas, Brian J. McEwen, Juan G. Nino-Vega, Gustavo Puccia, Rosana [UNIFESP] San-Blas, Gioconda Almeida Soares, Celia Maria de Birren, Bruce W. Cuomo, Christina A. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Broad Inst MIT & Harvard MIT Polish Acad Sci Universidade Federal de Goiás (UFG) Universidade de Brasília (UnB) Universidade de São Paulo (USP) Corp Invest Biol Inst Venezolano Invest Cient Universidade Federal de São Paulo (UNIFESP) Univ Vale Paraiba Lab Nacl Ciencia & Tecnol Bioetanol CTBE Univ Antioquia |
dc.contributor.author.fl_str_mv |
Desjardins, Christopher A. Champion, Mia D. Holder, Jason W. Muszewska, Anna Goldberg, Jonathan Bailao, Alexandre M. Brigido, Marcelo Macedo Silva Ferreira, Marcia Eliana da Garcia, Ana Maria Grynberg, Marcin Gujja, Sharvari Heiman, David I. Henn, Matthew R. Kodira, Chinnappa D. Leon-Narvaez, Henry Longo, Larissa V. G. [UNIFESP] Ma, Li-Jun Malavazi, Iran Matsuo, Alisson L. [UNIFESP] Morais, Flavia V. [UNIFESP] Pereira, Maristela Rodriguez-Brito, Sabrina Sakthikumar, Sharadha Salem-Izacc, Silvia M. Sykes, Sean M. Teixeira, Marcus Melo Vallejo, Milene C. [UNIFESP] Machado Telles Walter, Maria Emilia Yandava, Chandri Young, Sarah Zeng, Qiandong Zucker, Jeremy Felipe, Maria Sueli Goldman, Gustavo H. Haas, Brian J. McEwen, Juan G. Nino-Vega, Gustavo Puccia, Rosana [UNIFESP] San-Blas, Gioconda Almeida Soares, Celia Maria de Birren, Bruce W. Cuomo, Christina A. |
description |
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. in addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-01 2016-01-24T14:17:15Z 2016-01-24T14:17:15Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pgen.1002345 Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011. 10.1371/journal.pgen.1002345 WOS000296665400043.pdf 1553-7404 http://repositorio.unifesp.br/handle/11600/34080 WOS:000296665400043 |
url |
http://dx.doi.org/10.1371/journal.pgen.1002345 http://repositorio.unifesp.br/handle/11600/34080 |
identifier_str_mv |
Plos Genetics. San Francisco: Public Library Science, v. 7, n. 10, 16 p., 2011. 10.1371/journal.pgen.1002345 WOS000296665400043.pdf 1553-7404 WOS:000296665400043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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