Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2011.04.014 http://repositorio.unifesp.br/handle/11600/33720 |
Resumo: | Four novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved. |
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Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine waspsSolitary waspLinear cationic alpha-helical peptideAmphipathic alpha-helix structureAntimicrobial activityFour novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved.Toyama Univ, Inst Nat Med, Toyama 9300194, JapanButantan Inst, Immunopathol Lab, BR-05503900 São Paulo, BrazilSão Paulo State Univ, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilYamada Apiculture Ctr Inc, Okayama 7080393, JapanUtsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 3218505, JapanUniversidade Federal de São Paulo, Dept Biophys, Paulista Med Sch, BR-04044020 São Paulo, BrazilUniv Victoria, Genome BC Prote Ctr, Victoria, BC V8Z 7X8, CanadaButantan Inst, Bacteriol Lab, BR-05503900 São Paulo, BrazilNatl Inst Biomed Innovat, Res Ctr Med Plant Resources, Tsukuba, Ibaraki 3050843, JapanTokushima Bunri Univ, Fac Pharmaceut Sci, Kagawa 7692193, JapanUniversidade Federal de São Paulo, Dept Biophys, Paulista Med Sch, BR-04044020 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2008/00173-4CNPq: 307457/2008-7CNPq: 477507/2008-5Elsevier B.V.Toyama UnivButantan InstSão Paulo State UnivYamada Apiculture Ctr IncUtsunomiya UnivUniversidade Federal de São Paulo (UNIFESP)Univ VictoriaNatl Inst Biomed InnovatTokushima Bunri UnivRangel, MarisaSantos Cabrera, Marcia Perez dosKazuma, KoheiAndo, KenjiWang, XiaoyuKato, ManabuNihei, Ken-ichiHirata, Izaura Yoshico [UNIFESP]Cross, Tyra J.Garcia, Angelica NunesFaquim-Mauro, Eliana L.Franzolin, Marcia ReginaFuchino, HiroyukiMori-Yasumoto, KanamiSekita, SetsukoKadowaki, MakotoSatake, MotoyoshiKonno, Katsuhiro2016-01-24T14:16:47Z2016-01-24T14:16:47Z2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1081-1092application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2011.04.014Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011.10.1016/j.toxicon.2011.04.014WOS000291833400017.pdf0041-0101http://repositorio.unifesp.br/handle/11600/33720WOS:000291833400017engToxiconinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T20:26:17Zoai:repositorio.unifesp.br/:11600/33720Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T20:26:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
title |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
spellingShingle |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps Rangel, Marisa Solitary wasp Linear cationic alpha-helical peptide Amphipathic alpha-helix structure Antimicrobial activity |
title_short |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
title_full |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
title_fullStr |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
title_full_unstemmed |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
title_sort |
Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps |
author |
Rangel, Marisa |
author_facet |
Rangel, Marisa Santos Cabrera, Marcia Perez dos Kazuma, Kohei Ando, Kenji Wang, Xiaoyu Kato, Manabu Nihei, Ken-ichi Hirata, Izaura Yoshico [UNIFESP] Cross, Tyra J. Garcia, Angelica Nunes Faquim-Mauro, Eliana L. Franzolin, Marcia Regina Fuchino, Hiroyuki Mori-Yasumoto, Kanami Sekita, Setsuko Kadowaki, Makoto Satake, Motoyoshi Konno, Katsuhiro |
author_role |
author |
author2 |
Santos Cabrera, Marcia Perez dos Kazuma, Kohei Ando, Kenji Wang, Xiaoyu Kato, Manabu Nihei, Ken-ichi Hirata, Izaura Yoshico [UNIFESP] Cross, Tyra J. Garcia, Angelica Nunes Faquim-Mauro, Eliana L. Franzolin, Marcia Regina Fuchino, Hiroyuki Mori-Yasumoto, Kanami Sekita, Setsuko Kadowaki, Makoto Satake, Motoyoshi Konno, Katsuhiro |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Toyama Univ Butantan Inst São Paulo State Univ Yamada Apiculture Ctr Inc Utsunomiya Univ Universidade Federal de São Paulo (UNIFESP) Univ Victoria Natl Inst Biomed Innovat Tokushima Bunri Univ |
dc.contributor.author.fl_str_mv |
Rangel, Marisa Santos Cabrera, Marcia Perez dos Kazuma, Kohei Ando, Kenji Wang, Xiaoyu Kato, Manabu Nihei, Ken-ichi Hirata, Izaura Yoshico [UNIFESP] Cross, Tyra J. Garcia, Angelica Nunes Faquim-Mauro, Eliana L. Franzolin, Marcia Regina Fuchino, Hiroyuki Mori-Yasumoto, Kanami Sekita, Setsuko Kadowaki, Makoto Satake, Motoyoshi Konno, Katsuhiro |
dc.subject.por.fl_str_mv |
Solitary wasp Linear cationic alpha-helical peptide Amphipathic alpha-helix structure Antimicrobial activity |
topic |
Solitary wasp Linear cationic alpha-helical peptide Amphipathic alpha-helix structure Antimicrobial activity |
description |
Four novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-01 2016-01-24T14:16:47Z 2016-01-24T14:16:47Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2011.04.014 Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011. 10.1016/j.toxicon.2011.04.014 WOS000291833400017.pdf 0041-0101 http://repositorio.unifesp.br/handle/11600/33720 WOS:000291833400017 |
url |
http://dx.doi.org/10.1016/j.toxicon.2011.04.014 http://repositorio.unifesp.br/handle/11600/33720 |
identifier_str_mv |
Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011. 10.1016/j.toxicon.2011.04.014 WOS000291833400017.pdf 0041-0101 WOS:000291833400017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
1081-1092 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268304989093888 |