Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps

Detalhes bibliográficos
Autor(a) principal: Rangel, Marisa
Data de Publicação: 2011
Outros Autores: Santos Cabrera, Marcia Perez dos, Kazuma, Kohei, Ando, Kenji, Wang, Xiaoyu, Kato, Manabu, Nihei, Ken-ichi, Hirata, Izaura Yoshico [UNIFESP], Cross, Tyra J., Garcia, Angelica Nunes, Faquim-Mauro, Eliana L., Franzolin, Marcia Regina, Fuchino, Hiroyuki, Mori-Yasumoto, Kanami, Sekita, Setsuko, Kadowaki, Makoto, Satake, Motoyoshi, Konno, Katsuhiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.toxicon.2011.04.014
http://repositorio.unifesp.br/handle/11600/33720
Resumo: Four novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved.
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spelling Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine waspsSolitary waspLinear cationic alpha-helical peptideAmphipathic alpha-helix structureAntimicrobial activityFour novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved.Toyama Univ, Inst Nat Med, Toyama 9300194, JapanButantan Inst, Immunopathol Lab, BR-05503900 São Paulo, BrazilSão Paulo State Univ, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilYamada Apiculture Ctr Inc, Okayama 7080393, JapanUtsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 3218505, JapanUniversidade Federal de São Paulo, Dept Biophys, Paulista Med Sch, BR-04044020 São Paulo, BrazilUniv Victoria, Genome BC Prote Ctr, Victoria, BC V8Z 7X8, CanadaButantan Inst, Bacteriol Lab, BR-05503900 São Paulo, BrazilNatl Inst Biomed Innovat, Res Ctr Med Plant Resources, Tsukuba, Ibaraki 3050843, JapanTokushima Bunri Univ, Fac Pharmaceut Sci, Kagawa 7692193, JapanUniversidade Federal de São Paulo, Dept Biophys, Paulista Med Sch, BR-04044020 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2008/00173-4CNPq: 307457/2008-7CNPq: 477507/2008-5Elsevier B.V.Toyama UnivButantan InstSão Paulo State UnivYamada Apiculture Ctr IncUtsunomiya UnivUniversidade Federal de São Paulo (UNIFESP)Univ VictoriaNatl Inst Biomed InnovatTokushima Bunri UnivRangel, MarisaSantos Cabrera, Marcia Perez dosKazuma, KoheiAndo, KenjiWang, XiaoyuKato, ManabuNihei, Ken-ichiHirata, Izaura Yoshico [UNIFESP]Cross, Tyra J.Garcia, Angelica NunesFaquim-Mauro, Eliana L.Franzolin, Marcia ReginaFuchino, HiroyukiMori-Yasumoto, KanamiSekita, SetsukoKadowaki, MakotoSatake, MotoyoshiKonno, Katsuhiro2016-01-24T14:16:47Z2016-01-24T14:16:47Z2011-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1081-1092application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2011.04.014Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011.10.1016/j.toxicon.2011.04.014WOS000291833400017.pdf0041-0101http://repositorio.unifesp.br/handle/11600/33720WOS:000291833400017engToxiconinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T20:26:17Zoai:repositorio.unifesp.br/:11600/33720Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T20:26:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
title Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
spellingShingle Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
Rangel, Marisa
Solitary wasp
Linear cationic alpha-helical peptide
Amphipathic alpha-helix structure
Antimicrobial activity
title_short Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
title_full Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
title_fullStr Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
title_full_unstemmed Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
title_sort Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps
author Rangel, Marisa
author_facet Rangel, Marisa
Santos Cabrera, Marcia Perez dos
Kazuma, Kohei
Ando, Kenji
Wang, Xiaoyu
Kato, Manabu
Nihei, Ken-ichi
Hirata, Izaura Yoshico [UNIFESP]
Cross, Tyra J.
Garcia, Angelica Nunes
Faquim-Mauro, Eliana L.
Franzolin, Marcia Regina
Fuchino, Hiroyuki
Mori-Yasumoto, Kanami
Sekita, Setsuko
Kadowaki, Makoto
Satake, Motoyoshi
Konno, Katsuhiro
author_role author
author2 Santos Cabrera, Marcia Perez dos
Kazuma, Kohei
Ando, Kenji
Wang, Xiaoyu
Kato, Manabu
Nihei, Ken-ichi
Hirata, Izaura Yoshico [UNIFESP]
Cross, Tyra J.
Garcia, Angelica Nunes
Faquim-Mauro, Eliana L.
Franzolin, Marcia Regina
Fuchino, Hiroyuki
Mori-Yasumoto, Kanami
Sekita, Setsuko
Kadowaki, Makoto
Satake, Motoyoshi
Konno, Katsuhiro
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Toyama Univ
Butantan Inst
São Paulo State Univ
Yamada Apiculture Ctr Inc
Utsunomiya Univ
Universidade Federal de São Paulo (UNIFESP)
Univ Victoria
Natl Inst Biomed Innovat
Tokushima Bunri Univ
dc.contributor.author.fl_str_mv Rangel, Marisa
Santos Cabrera, Marcia Perez dos
Kazuma, Kohei
Ando, Kenji
Wang, Xiaoyu
Kato, Manabu
Nihei, Ken-ichi
Hirata, Izaura Yoshico [UNIFESP]
Cross, Tyra J.
Garcia, Angelica Nunes
Faquim-Mauro, Eliana L.
Franzolin, Marcia Regina
Fuchino, Hiroyuki
Mori-Yasumoto, Kanami
Sekita, Setsuko
Kadowaki, Makoto
Satake, Motoyoshi
Konno, Katsuhiro
dc.subject.por.fl_str_mv Solitary wasp
Linear cationic alpha-helical peptide
Amphipathic alpha-helix structure
Antimicrobial activity
topic Solitary wasp
Linear cationic alpha-helical peptide
Amphipathic alpha-helix structure
Antimicrobial activity
description Four novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. in fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. in the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011-06-01
2016-01-24T14:16:47Z
2016-01-24T14:16:47Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.toxicon.2011.04.014
Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011.
10.1016/j.toxicon.2011.04.014
WOS000291833400017.pdf
0041-0101
http://repositorio.unifesp.br/handle/11600/33720
WOS:000291833400017
url http://dx.doi.org/10.1016/j.toxicon.2011.04.014
http://repositorio.unifesp.br/handle/11600/33720
identifier_str_mv Toxicon. Oxford: Pergamon-Elsevier B.V., v. 57, n. 7-8, p. 1081-1092, 2011.
10.1016/j.toxicon.2011.04.014
WOS000291833400017.pdf
0041-0101
WOS:000291833400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Toxicon
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 1081-1092
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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